Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02645

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyciQ and yciO are separated by 27 nucleotidesyciO and yciV overlap by 4 nucleotidesyciV and trpL are separated by 137 nucleotidestrpL and trpE are separated by 91 nucleotides OKFHMN_02635: yciQ - Uncharacterized protein YciQ, at 417,566 to 419,149 yciQ OKFHMN_02640: yciO - L-threonylcarbamoyladenylate synthase, at 419,177 to 419,797 yciO OKFHMN_02645: yciV - 5'-3' exoribonuclease, at 419,794 to 420,675 yciV OKFHMN_02650: trpL - trp operon leader peptide, at 420,813 to 420,857 trpL OKFHMN_02655: trpE - anthranilate synthase component I, at 420,949 to 422,511 trpE Position (kb) 419 420 421Strain fitness (log2 ratio) -2 -1 0 1at 418.923 kb on - strand, within yciQat 419.059 kb on - strandat 419.059 kb on - strandat 419.091 kb on - strandat 419.115 kb on - strandat 419.181 kb on - strandat 419.507 kb on - strand, within yciOat 419.509 kb on - strand, within yciOat 419.968 kb on - strand, within yciVat 420.089 kb on - strand, within yciVat 420.141 kb on + strand, within yciVat 420.308 kb on - strand, within yciVat 420.308 kb on - strand, within yciVat 420.427 kb on - strand, within yciVat 420.829 kb on + strand, within trpLat 420.882 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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418,923 - yciQ OKFHMN_02635 0.86 -0.3
419,059 - +1.2
419,059 - -1.0
419,091 - +0.6
419,115 - -0.8
419,181 - -2.0
419,507 - yciO OKFHMN_02640 0.53 +1.2
419,509 - yciO OKFHMN_02640 0.53 +0.2
419,968 - yciV OKFHMN_02645 0.20 +0.7
420,089 - yciV OKFHMN_02645 0.33 +0.0
420,141 + yciV OKFHMN_02645 0.39 +1.6
420,308 - yciV OKFHMN_02645 0.58 +1.0
420,308 - yciV OKFHMN_02645 0.58 -0.4
420,427 - yciV OKFHMN_02645 0.72 -0.3
420,829 + trpL OKFHMN_02650 0.36 +0.7
420,882 + +1.6

Or see this region's nucleotide sequence