Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02495

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttolC and adeB are separated by 22 nucleotidesadeB and acrA overlap by 1 nucleotides OKFHMN_02490: tolC - multidrug transporter, at 386,598 to 387,752 tolC OKFHMN_02495: adeB - hydrophobe/amphiphile efflux-1 family RND transporter, at 387,775 to 390,858 adeB OKFHMN_02500: acrA - efflux transporter periplasmic adaptor subunit, at 390,858 to 391,979 acrA Position (kb) 387 388 389 390 391Strain fitness (log2 ratio) -2 -1 0 1at 387.489 kb on - strand, within tolCat 387.735 kb on - strandat 387.752 kb on + strandat 387.757 kb on + strandat 387.828 kb on - strandat 387.839 kb on + strandat 387.967 kb on + strandat 388.425 kb on - strand, within adeBat 388.678 kb on - strand, within adeBat 388.690 kb on - strand, within adeBat 388.690 kb on - strand, within adeBat 388.793 kb on - strand, within adeBat 388.818 kb on + strand, within adeBat 389.244 kb on - strand, within adeBat 389.497 kb on - strand, within adeBat 389.795 kb on + strand, within adeBat 390.039 kb on - strand, within adeBat 390.205 kb on + strand, within adeBat 390.710 kb on + strandat 391.496 kb on + strand, within acrAat 391.801 kb on - strand, within acrA

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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387,489 - tolC OKFHMN_02490 0.77 +1.6
387,735 - +0.6
387,752 + +0.1
387,757 + +0.1
387,828 - +0.1
387,839 + -0.8
387,967 + +0.3
388,425 - adeB OKFHMN_02495 0.21 +0.9
388,678 - adeB OKFHMN_02495 0.29 +1.3
388,690 - adeB OKFHMN_02495 0.30 -0.4
388,690 - adeB OKFHMN_02495 0.30 +0.9
388,793 - adeB OKFHMN_02495 0.33 -0.8
388,818 + adeB OKFHMN_02495 0.34 -0.0
389,244 - adeB OKFHMN_02495 0.48 +0.2
389,497 - adeB OKFHMN_02495 0.56 +0.6
389,795 + adeB OKFHMN_02495 0.65 -1.2
390,039 - adeB OKFHMN_02495 0.73 +0.2
390,205 + adeB OKFHMN_02495 0.79 -0.0
390,710 + -1.4
391,496 + acrA OKFHMN_02500 0.57 -1.8
391,801 - acrA OKFHMN_02500 0.84 -0.3

Or see this region's nucleotide sequence