Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00415

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrepA2 and OKFHMN_00410 are separated by 239 nucleotidesOKFHMN_00410 and OKFHMN_00415 are separated by 37 nucleotidesOKFHMN_00415 and yncB are separated by 45 nucleotidesyncB and OKFHMN_00430 are separated by 201 nucleotides OKFHMN_00405: repA2 - Replication regulatory protein repA2, at 86,042 to 86,296 repA2 OKFHMN_00410: OKFHMN_00410 - W0003, at 86,536 to 86,874 _00410 OKFHMN_00415: OKFHMN_00415 - transcriptional regulator, at 86,912 to 87,121 _00415 OKFHMN_00420: yncB - Uncharacterized endonuclease, at 87,167 to 87,628 yncB OKFHMN_00430: OKFHMN_00430 - hypothetical protein, at 87,830 to 88,081 _00430 Position (kb) 86 87 88Strain fitness (log2 ratio) -2 -1 0 1 2at 85.925 kb on + strandat 85.925 kb on + strandat 86.002 kb on - strandat 86.140 kb on + strand, within repA2at 86.141 kb on - strand, within repA2at 86.141 kb on - strand, within repA2at 86.141 kb on - strand, within repA2at 86.141 kb on - strand, within repA2at 86.142 kb on + strand, within repA2at 86.142 kb on + strand, within repA2at 86.143 kb on - strand, within repA2at 86.143 kb on - strand, within repA2at 86.143 kb on - strand, within repA2at 86.143 kb on - strand, within repA2at 86.160 kb on - strand, within repA2at 86.160 kb on - strand, within repA2at 86.188 kb on + strand, within repA2at 86.201 kb on + strand, within repA2at 86.244 kb on + strand, within repA2at 86.245 kb on - strand, within repA2at 86.311 kb on - strandat 86.314 kb on + strandat 86.314 kb on + strandat 86.315 kb on - strandat 86.343 kb on - strandat 86.414 kb on - strandat 86.423 kb on + strandat 86.434 kb on - strandat 86.445 kb on + strandat 86.445 kb on + strandat 86.445 kb on + strandat 86.446 kb on - strandat 86.446 kb on - strandat 86.541 kb on + strandat 86.542 kb on - strandat 86.640 kb on + strand, within OKFHMN_00410at 86.641 kb on - strand, within OKFHMN_00410at 86.699 kb on - strand, within OKFHMN_00410at 86.715 kb on - strand, within OKFHMN_00410at 86.755 kb on + strand, within OKFHMN_00410at 86.828 kb on - strand, within OKFHMN_00410at 87.279 kb on + strand, within yncBat 87.288 kb on + strand, within yncBat 87.289 kb on - strand, within yncBat 87.291 kb on - strand, within yncBat 87.316 kb on + strand, within yncBat 87.317 kb on - strand, within yncBat 87.454 kb on - strand, within yncBat 87.481 kb on + strand, within yncBat 87.482 kb on - strand, within yncBat 87.546 kb on - strand, within yncBat 87.569 kb on - strand, within yncBat 87.619 kb on - strandat 87.747 kb on + strandat 88.090 kb on - strandat 88.102 kb on + strandat 88.113 kb on + strandat 88.116 kb on + strandat 88.116 kb on + strandat 88.116 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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85,925 + -0.7
85,925 + +0.9
86,002 - +0.1
86,140 + repA2 OKFHMN_00405 0.38 +0.5
86,141 - repA2 OKFHMN_00405 0.39 -1.6
86,141 - repA2 OKFHMN_00405 0.39 -0.4
86,141 - repA2 OKFHMN_00405 0.39 +0.3
86,141 - repA2 OKFHMN_00405 0.39 -0.8
86,142 + repA2 OKFHMN_00405 0.39 +0.4
86,142 + repA2 OKFHMN_00405 0.39 +1.2
86,143 - repA2 OKFHMN_00405 0.40 +0.1
86,143 - repA2 OKFHMN_00405 0.40 +2.7
86,143 - repA2 OKFHMN_00405 0.40 -0.3
86,143 - repA2 OKFHMN_00405 0.40 -0.3
86,160 - repA2 OKFHMN_00405 0.46 +0.1
86,160 - repA2 OKFHMN_00405 0.46 -0.7
86,188 + repA2 OKFHMN_00405 0.57 +0.1
86,201 + repA2 OKFHMN_00405 0.62 -0.6
86,244 + repA2 OKFHMN_00405 0.79 +1.1
86,245 - repA2 OKFHMN_00405 0.80 +1.3
86,311 - -0.4
86,314 + +2.1
86,314 + -2.5
86,315 - -0.7
86,343 - +0.1
86,414 - +0.3
86,423 + -1.0
86,434 - -0.2
86,445 + -0.7
86,445 + -0.0
86,445 + -1.1
86,446 - -0.1
86,446 - -0.5
86,541 + -0.1
86,542 - +0.0
86,640 + OKFHMN_00410 0.31 +0.2
86,641 - OKFHMN_00410 0.31 -1.7
86,699 - OKFHMN_00410 0.48 +0.8
86,715 - OKFHMN_00410 0.53 +0.5
86,755 + OKFHMN_00410 0.65 +0.3
86,828 - OKFHMN_00410 0.86 -0.5
87,279 + yncB OKFHMN_00420 0.24 -0.7
87,288 + yncB OKFHMN_00420 0.26 -0.4
87,289 - yncB OKFHMN_00420 0.26 +1.6
87,291 - yncB OKFHMN_00420 0.27 -0.7
87,316 + yncB OKFHMN_00420 0.32 +1.4
87,317 - yncB OKFHMN_00420 0.32 +0.6
87,454 - yncB OKFHMN_00420 0.62 -0.9
87,481 + yncB OKFHMN_00420 0.68 +0.6
87,482 - yncB OKFHMN_00420 0.68 -1.3
87,546 - yncB OKFHMN_00420 0.82 -0.3
87,569 - yncB OKFHMN_00420 0.87 +0.5
87,619 - -1.6
87,747 + +1.1
88,090 - +0.9
88,102 + -0.3
88,113 + -0.4
88,116 + -0.0
88,116 + -2.2
88,116 + -0.1

Or see this region's nucleotide sequence