Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27815

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynfA and OKFHMN_27805 are separated by 205 nucleotidesOKFHMN_27805 and OKFHMN_27810 are separated by 500 nucleotidesOKFHMN_27810 and OKFHMN_27815 are separated by 34 nucleotidesOKFHMN_27815 and dnaQ are separated by 87 nucleotides OKFHMN_27800: ynfA - YnfA family protein, at 5,354,945 to 5,355,271 ynfA OKFHMN_27805: OKFHMN_27805 - Starvation-sensing protein, at 5,355,477 to 5,355,569 _27805 OKFHMN_27810: OKFHMN_27810 - Integrase, cryptic prophage CP-933P, at 5,356,070 to 5,356,225 _27810 OKFHMN_27815: OKFHMN_27815 - DNA-binding protein, at 5,356,260 to 5,356,496 _27815 OKFHMN_27820: dnaQ - exonuclease, at 5,356,584 to 5,359,055 dnaQ Position (kb) 5356 5357Strain fitness (log2 ratio) -1 0 1at 5355.985 kb on - strandat 5356.181 kb on - strand, within OKFHMN_27810at 5356.359 kb on - strand, within OKFHMN_27815at 5356.380 kb on - strand, within OKFHMN_27815at 5356.555 kb on + strandat 5357.268 kb on - strand, within dnaQ

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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5,355,985 - -0.6
5,356,181 - OKFHMN_27810 0.71 -0.1
5,356,359 - OKFHMN_27815 0.42 +1.1
5,356,380 - OKFHMN_27815 0.51 -0.5
5,356,555 + -0.1
5,357,268 - dnaQ OKFHMN_27820 0.28 -1.0

Or see this region's nucleotide sequence