Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nudB and OKFHMN_26255 are separated by 28 nucleotides OKFHMN_26255 and OKFHMN_26260 overlap by 1 nucleotides OKFHMN_26260 and ruvC overlap by 1 nucleotides ruvC and OKFHMN_26270 are separated by 1 nucleotides OKFHMN_26270 and yobI are separated by 70 nucleotides yobI and ruvA are separated by 138 nucleotides
OKFHMN_26250: nudB - dihydroneopterin triphosphate diphosphatase, at 5,059,800 to 5,060,309
nudB
OKFHMN_26255: OKFHMN_26255 - putative transcriptional regulatory protein YebC, at 5,060,338 to 5,061,078
_26255
OKFHMN_26260: OKFHMN_26260 - Crossover junction endodeoxyribonuclease RuvC, at 5,061,078 to 5,061,113
_26260
OKFHMN_26265: ruvC - crossover junction endodeoxyribonuclease RuvC, at 5,061,113 to 5,061,634
ruvC
OKFHMN_26270: OKFHMN_26270 - Protein, at 5,061,636 to 5,062,238
_26270
OKFHMN_26275: yobI - Uncharacterized protein YobI, at 5,062,309 to 5,062,374
yobI
OKFHMN_26280: ruvA - Holliday junction branch migration protein RuvA, at 5,062,513 to 5,063,124
ruvA
Position (kb) 5061
5062 Strain fitness (log2 ratio) -1
0
1
2 at 5060.116 kb on + strand, within nudB at 5060.293 kb on + strand at 5060.415 kb on + strand, within OKFHMN_26255 at 5060.830 kb on + strand, within OKFHMN_26255 at 5060.854 kb on + strand, within OKFHMN_26255 at 5060.854 kb on + strand, within OKFHMN_26255 at 5061.637 kb on + strand at 5061.637 kb on + strand at 5061.861 kb on - strand, within OKFHMN_26270 at 5062.455 kb on + strand at 5062.475 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 5,060,116 + nudB OKFHMN_26250 0.62 -1.1 5,060,293 + +1.2 5,060,415 + OKFHMN_26255 0.10 +1.0 5,060,830 + OKFHMN_26255 0.66 -1.4 5,060,854 + OKFHMN_26255 0.70 +0.7 5,060,854 + OKFHMN_26255 0.70 -0.3 5,061,637 + -0.3 5,061,637 + -0.9 5,061,861 - OKFHMN_26270 0.37 +2.3 5,062,455 + +1.7 5,062,475 + -0.0
Or see this region's nucleotide sequence