Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25815

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsdiA and yecF are separated by 67 nucleotidesyecF and uvrC are separated by 458 nucleotides OKFHMN_25810: sdiA - transcriptional regulator SdiA, at 4,990,002 to 4,990,724 sdiA OKFHMN_25815: yecF - DUF2594 domain-containing protein, at 4,990,792 to 4,991,016 yecF OKFHMN_25820: uvrC - excinuclease ABC subunit UvrC, at 4,991,475 to 4,992,131 uvrC Position (kb) 4990 4991 4992Strain fitness (log2 ratio) -1 0 1 2at 4989.929 kb on + strandat 4989.963 kb on + strandat 4990.122 kb on + strand, within sdiAat 4990.279 kb on + strand, within sdiAat 4990.439 kb on + strand, within sdiAat 4990.439 kb on + strand, within sdiAat 4990.709 kb on + strandat 4990.889 kb on - strand, within yecFat 4990.889 kb on - strand, within yecFat 4991.010 kb on + strandat 4991.137 kb on + strandat 4991.149 kb on + strandat 4991.153 kb on - strandat 4991.290 kb on - strandat 4991.333 kb on + strandat 4991.543 kb on + strand, within uvrCat 4991.545 kb on + strand, within uvrCat 4991.594 kb on + strand, within uvrCat 4991.717 kb on + strand, within uvrCat 4991.740 kb on + strand, within uvrC

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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4,989,929 + +1.0
4,989,963 + -0.5
4,990,122 + sdiA OKFHMN_25810 0.17 +0.1
4,990,279 + sdiA OKFHMN_25810 0.38 +0.3
4,990,439 + sdiA OKFHMN_25810 0.60 +1.6
4,990,439 + sdiA OKFHMN_25810 0.60 +1.1
4,990,709 + -1.7
4,990,889 - yecF OKFHMN_25815 0.43 +0.0
4,990,889 - yecF OKFHMN_25815 0.43 +0.2
4,991,010 + +2.1
4,991,137 + -0.1
4,991,149 + +0.9
4,991,153 - +0.1
4,991,290 - +0.9
4,991,333 + +0.3
4,991,543 + uvrC OKFHMN_25820 0.10 -0.8
4,991,545 + uvrC OKFHMN_25820 0.11 +0.7
4,991,594 + uvrC OKFHMN_25820 0.18 +0.4
4,991,717 + uvrC OKFHMN_25820 0.37 +0.3
4,991,740 + uvrC OKFHMN_25820 0.40 -0.9

Or see this region's nucleotide sequence