Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24810

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntstxA2c and stxB2c are separated by 11 nucleotidesstxB2c and iraM are separated by 296 nucleotidesiraM and OKFHMN_24825 are separated by 243 nucleotides OKFHMN_24805: stxA2c - Shiga toxin Stx2c subunit A, at 4,821,867 to 4,822,826 stxA2c OKFHMN_24810: stxB2c - Shiga toxin Stx2c subunit B, at 4,822,838 to 4,823,107 stxB2c OKFHMN_24820: iraM - Anti-adapter protein IraM, at 4,823,404 to 4,823,727 iraM OKFHMN_24825: OKFHMN_24825 - sialate O-acetylesterase, at 4,823,971 to 4,825,908 _24825 Position (kb) 4822 4823 4824Strain fitness (log2 ratio) -1 0 1 2 3at 4821.865 kb on - strandat 4821.865 kb on - strandat 4821.987 kb on - strand, within stxA2cat 4822.022 kb on + strand, within stxA2cat 4822.224 kb on - strand, within stxA2cat 4822.225 kb on + strand, within stxA2cat 4822.225 kb on + strand, within stxA2cat 4822.273 kb on - strand, within stxA2cat 4822.376 kb on + strand, within stxA2cat 4822.376 kb on + strand, within stxA2cat 4822.377 kb on - strand, within stxA2cat 4822.751 kb on + strandat 4822.770 kb on - strandat 4823.127 kb on + strandat 4823.250 kb on - strandat 4823.338 kb on + strandat 4823.338 kb on + strandat 4823.339 kb on - strandat 4823.355 kb on - strandat 4823.543 kb on + strand, within iraMat 4823.544 kb on - strand, within iraMat 4823.659 kb on - strand, within iraMat 4823.979 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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4,821,865 - +1.3
4,821,865 - +1.2
4,821,987 - stxA2c OKFHMN_24805 0.12 +0.2
4,822,022 + stxA2c OKFHMN_24805 0.16 -0.9
4,822,224 - stxA2c OKFHMN_24805 0.37 +1.5
4,822,225 + stxA2c OKFHMN_24805 0.37 -0.5
4,822,225 + stxA2c OKFHMN_24805 0.37 -1.0
4,822,273 - stxA2c OKFHMN_24805 0.42 -0.0
4,822,376 + stxA2c OKFHMN_24805 0.53 -0.9
4,822,376 + stxA2c OKFHMN_24805 0.53 +0.8
4,822,377 - stxA2c OKFHMN_24805 0.53 -0.1
4,822,751 + -0.9
4,822,770 - +1.3
4,823,127 + +0.7
4,823,250 - +2.1
4,823,338 + -0.3
4,823,338 + -0.1
4,823,339 - -1.3
4,823,355 - +2.0
4,823,543 + iraM OKFHMN_24820 0.43 +3.7
4,823,544 - iraM OKFHMN_24820 0.43 +0.4
4,823,659 - iraM OKFHMN_24820 0.79 +1.7
4,823,979 + +1.0

Or see this region's nucleotide sequence