Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheA and OKFHMN_21230 are separated by 98 nucleotides OKFHMN_21230 and raiA are separated by 103 nucleotides raiA and OKFHMN_21240 are separated by 5 nucleotides OKFHMN_21240 and bamD are separated by 229 nucleotides bamD and rluD are separated by 134 nucleotides
OKFHMN_21225: pheA - bifunctional chorismate mutase/prephenate dehydratase, at 4,115,555 to 4,116,715
pheA
OKFHMN_21230: OKFHMN_21230 - Leader peptide of chorismate mutase-P-prephenate dehydratase, at 4,116,814 to 4,116,861
_21230
OKFHMN_21235: raiA - ribosome-associated translation inhibitor RaiA, at 4,116,965 to 4,117,306
raiA
OKFHMN_21240: OKFHMN_21240 - Leader peptide, at 4,117,312 to 4,117,347
_21240
OKFHMN_21245: bamD - outer membrane protein assembly factor BamD, at 4,117,577 to 4,118,314
bamD
OKFHMN_21250: rluD - 23S rRNA pseudouridine(1911/1915/1917) synthase RluD, at 4,118,449 to 4,119,429
rluD
Position (kb)
4117
4118
4119 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4116.715 kb on + strand at 4116.716 kb on - strand at 4116.716 kb on - strand at 4116.739 kb on - strand at 4116.887 kb on - strand at 4116.891 kb on + strand at 4116.948 kb on - strand at 4116.956 kb on + strand at 4116.959 kb on - strand at 4116.966 kb on + strand at 4117.095 kb on + strand, within raiA at 4117.096 kb on - strand, within raiA at 4117.096 kb on - strand, within raiA at 4117.096 kb on - strand, within raiA at 4117.100 kb on - strand, within raiA at 4117.111 kb on + strand, within raiA at 4117.192 kb on + strand, within raiA at 4117.193 kb on - strand, within raiA at 4117.193 kb on - strand, within raiA at 4117.193 kb on - strand, within raiA at 4117.292 kb on - strand at 4117.307 kb on - strand at 4117.459 kb on + strand at 4117.476 kb on + strand at 4117.499 kb on + strand at 4117.582 kb on + strand at 4117.582 kb on + strand at 4117.582 kb on + strand at 4117.583 kb on - strand at 4117.614 kb on - strand at 4118.388 kb on - strand at 4118.388 kb on - strand at 4118.388 kb on - strand at 4118.395 kb on + strand at 4118.395 kb on + strand at 4118.395 kb on + strand at 4118.396 kb on - strand at 4118.397 kb on + strand at 4118.398 kb on - strand at 4118.398 kb on - strand at 4118.400 kb on + strand at 4118.401 kb on - strand at 4118.445 kb on - strand at 4118.445 kb on - strand at 4118.445 kb on - strand at 4118.446 kb on + strand at 4118.446 kb on + strand at 4118.446 kb on + strand at 4118.446 kb on + strand at 4118.446 kb on + strand at 4118.446 kb on + strand at 4118.446 kb on + strand at 4118.448 kb on + strand at 4118.448 kb on + strand at 4118.449 kb on - strand at 4118.449 kb on - strand at 4118.508 kb on - strand at 4118.624 kb on + strand, within rluD at 4118.724 kb on + strand, within rluD at 4118.724 kb on + strand, within rluD at 4118.725 kb on - strand, within rluD at 4118.739 kb on + strand, within rluD at 4118.759 kb on + strand, within rluD at 4118.759 kb on + strand, within rluD at 4118.759 kb on + strand, within rluD at 4118.760 kb on - strand, within rluD at 4118.805 kb on - strand, within rluD at 4118.813 kb on - strand, within rluD at 4118.813 kb on - strand, within rluD at 4118.815 kb on + strand, within rluD at 4118.997 kb on + strand, within rluD at 4119.002 kb on + strand, within rluD at 4119.003 kb on - strand, within rluD at 4119.036 kb on + strand, within rluD at 4119.144 kb on + strand, within rluD at 4119.144 kb on + strand, within rluD at 4119.145 kb on - strand, within rluD at 4119.165 kb on + strand, within rluD at 4119.206 kb on - strand, within rluD
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 4,116,715 + -0.9 4,116,716 - +0.7 4,116,716 - -1.4 4,116,739 - +0.2 4,116,887 - +0.2 4,116,891 + -1.6 4,116,948 - +0.0 4,116,956 + -2.2 4,116,959 - +0.1 4,116,966 + +0.5 4,117,095 + raiA OKFHMN_21235 0.38 -0.1 4,117,096 - raiA OKFHMN_21235 0.38 +0.0 4,117,096 - raiA OKFHMN_21235 0.38 +0.4 4,117,096 - raiA OKFHMN_21235 0.38 +0.3 4,117,100 - raiA OKFHMN_21235 0.39 -0.3 4,117,111 + raiA OKFHMN_21235 0.43 +0.4 4,117,192 + raiA OKFHMN_21235 0.66 +0.5 4,117,193 - raiA OKFHMN_21235 0.67 +0.3 4,117,193 - raiA OKFHMN_21235 0.67 -0.7 4,117,193 - raiA OKFHMN_21235 0.67 +1.9 4,117,292 - -1.1 4,117,307 - -2.0 4,117,459 + -1.7 4,117,476 + +1.3 4,117,499 + -0.8 4,117,582 + +0.8 4,117,582 + +0.9 4,117,582 + +0.5 4,117,583 - -0.7 4,117,614 - +0.6 4,118,388 - -0.5 4,118,388 - +1.7 4,118,388 - -0.6 4,118,395 + -0.5 4,118,395 + -3.1 4,118,395 + +0.1 4,118,396 - +0.8 4,118,397 + +1.7 4,118,398 - +1.4 4,118,398 - +1.6 4,118,400 + +1.6 4,118,401 - +0.1 4,118,445 - +0.7 4,118,445 - +1.0 4,118,445 - -0.1 4,118,446 + +2.9 4,118,446 + +0.4 4,118,446 + -0.7 4,118,446 + +0.1 4,118,446 + +0.8 4,118,446 + -0.2 4,118,446 + +1.1 4,118,448 + +0.3 4,118,448 + +1.9 4,118,449 - +1.7 4,118,449 - +0.4 4,118,508 - +0.3 4,118,624 + rluD OKFHMN_21250 0.18 +0.1 4,118,724 + rluD OKFHMN_21250 0.28 +0.9 4,118,724 + rluD OKFHMN_21250 0.28 +0.8 4,118,725 - rluD OKFHMN_21250 0.28 +1.5 4,118,739 + rluD OKFHMN_21250 0.30 +0.3 4,118,759 + rluD OKFHMN_21250 0.32 -0.7 4,118,759 + rluD OKFHMN_21250 0.32 -0.3 4,118,759 + rluD OKFHMN_21250 0.32 -0.2 4,118,760 - rluD OKFHMN_21250 0.32 +1.1 4,118,805 - rluD OKFHMN_21250 0.36 -0.6 4,118,813 - rluD OKFHMN_21250 0.37 -0.1 4,118,813 - rluD OKFHMN_21250 0.37 +0.7 4,118,815 + rluD OKFHMN_21250 0.37 +1.0 4,118,997 + rluD OKFHMN_21250 0.56 +0.9 4,119,002 + rluD OKFHMN_21250 0.56 +0.8 4,119,003 - rluD OKFHMN_21250 0.56 +1.6 4,119,036 + rluD OKFHMN_21250 0.60 +0.0 4,119,144 + rluD OKFHMN_21250 0.71 -0.2 4,119,144 + rluD OKFHMN_21250 0.71 +1.4 4,119,145 - rluD OKFHMN_21250 0.71 -1.2 4,119,165 + rluD OKFHMN_21250 0.73 +0.8 4,119,206 - rluD OKFHMN_21250 0.77 +0.7
Or see this region's nucleotide sequence