Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20345

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygcR and fixB overlap by 4 nucleotidesfixB and OKFHMN_20345 are separated by 15 nucleotidesOKFHMN_20345 and ygcP are separated by 147 nucleotidesygcP and fixX are separated by 16 nucleotidesfixX and ygcN overlap by 10 nucleotides OKFHMN_20335: ygcR - Putative electron transfer flavoprotein subunit YgcR, at 3,959,716 to 3,960,495 ygcR OKFHMN_20340: fixB - electron transfer flavoprotein, at 3,960,492 to 3,961,061 fixB OKFHMN_20345: OKFHMN_20345 - Electron transfer flavoprotein subunit YgcQ, at 3,961,077 to 3,961,352 _20345 OKFHMN_20350: ygcP - Uncharacterized protein YgcP, at 3,961,500 to 3,962,075 ygcP OKFHMN_20355: fixX - ferredoxin family protein, at 3,962,092 to 3,962,352 fixX OKFHMN_20360: ygcN - putative electron transfer flavoprotein-quinone oxidoreductase YgcN, at 3,962,343 to 3,963,614 ygcN Position (kb) 3961 3962Strain fitness (log2 ratio) -2 -1 0 1 2at 3960.210 kb on + strand, within ygcRat 3960.210 kb on + strand, within ygcRat 3960.458 kb on + strandat 3960.573 kb on - strand, within fixBat 3960.592 kb on + strand, within fixBat 3960.776 kb on - strand, within fixBat 3960.842 kb on - strand, within fixBat 3960.842 kb on - strand, within fixBat 3960.952 kb on + strand, within fixBat 3960.952 kb on + strand, within fixBat 3960.953 kb on - strand, within fixBat 3960.953 kb on - strand, within fixBat 3961.084 kb on - strandat 3961.169 kb on + strand, within OKFHMN_20345at 3961.202 kb on - strand, within OKFHMN_20345at 3961.220 kb on + strand, within OKFHMN_20345at 3961.241 kb on + strand, within OKFHMN_20345at 3961.398 kb on + strandat 3961.399 kb on - strandat 3961.407 kb on + strandat 3961.417 kb on + strandat 3961.461 kb on + strandat 3961.487 kb on + strandat 3961.715 kb on + strand, within ygcPat 3961.716 kb on - strand, within ygcPat 3961.748 kb on + strand, within ygcPat 3961.869 kb on - strand, within ygcPat 3961.909 kb on + strand, within ygcPat 3961.909 kb on + strand, within ygcPat 3961.966 kb on + strand, within ygcPat 3961.967 kb on - strand, within ygcPat 3961.980 kb on - strand, within ygcPat 3962.064 kb on + strandat 3962.089 kb on + strandat 3962.090 kb on - strandat 3962.147 kb on - strand, within fixXat 3962.201 kb on + strand, within fixXat 3962.201 kb on + strand, within fixXat 3962.202 kb on - strand, within fixXat 3962.246 kb on - strand, within fixXat 3962.272 kb on + strand, within fixXat 3962.273 kb on - strand, within fixXat 3962.345 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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3,960,210 + ygcR OKFHMN_20335 0.63 +0.9
3,960,210 + ygcR OKFHMN_20335 0.63 +0.2
3,960,458 + -0.1
3,960,573 - fixB OKFHMN_20340 0.14 -0.1
3,960,592 + fixB OKFHMN_20340 0.18 +0.4
3,960,776 - fixB OKFHMN_20340 0.50 -0.1
3,960,842 - fixB OKFHMN_20340 0.61 -0.9
3,960,842 - fixB OKFHMN_20340 0.61 +0.3
3,960,952 + fixB OKFHMN_20340 0.81 -0.3
3,960,952 + fixB OKFHMN_20340 0.81 +0.6
3,960,953 - fixB OKFHMN_20340 0.81 +0.5
3,960,953 - fixB OKFHMN_20340 0.81 -0.6
3,961,084 - -0.4
3,961,169 + OKFHMN_20345 0.33 +0.1
3,961,202 - OKFHMN_20345 0.45 +1.9
3,961,220 + OKFHMN_20345 0.52 -0.2
3,961,241 + OKFHMN_20345 0.59 -0.6
3,961,398 + -0.5
3,961,399 - +0.1
3,961,407 + -0.1
3,961,417 + +0.3
3,961,461 + +0.2
3,961,487 + -1.5
3,961,715 + ygcP OKFHMN_20350 0.37 +0.8
3,961,716 - ygcP OKFHMN_20350 0.38 -0.6
3,961,748 + ygcP OKFHMN_20350 0.43 -0.5
3,961,869 - ygcP OKFHMN_20350 0.64 -0.6
3,961,909 + ygcP OKFHMN_20350 0.71 +1.2
3,961,909 + ygcP OKFHMN_20350 0.71 +0.0
3,961,966 + ygcP OKFHMN_20350 0.81 -0.4
3,961,967 - ygcP OKFHMN_20350 0.81 -1.6
3,961,980 - ygcP OKFHMN_20350 0.83 +0.5
3,962,064 + +0.4
3,962,089 + +0.8
3,962,090 - +0.8
3,962,147 - fixX OKFHMN_20355 0.21 -1.9
3,962,201 + fixX OKFHMN_20355 0.42 +0.9
3,962,201 + fixX OKFHMN_20355 0.42 +0.2
3,962,202 - fixX OKFHMN_20355 0.42 +0.7
3,962,246 - fixX OKFHMN_20355 0.59 +0.1
3,962,272 + fixX OKFHMN_20355 0.69 +0.4
3,962,273 - fixX OKFHMN_20355 0.69 -0.8
3,962,345 - +0.6

Or see this region's nucleotide sequence