Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20035

Experiment: EV146

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntppdA and pulJ overlap by 10 nucleotidespulJ and ygdB overlap by 4 nucleotidesygdB and ppdC overlap by 16 nucleotidesppdC and recC are separated by 12 nucleotides OKFHMN_20020: ppdA - prepilin peptidase-dependent protein, at 3,889,813 to 3,890,283 ppdA OKFHMN_20025: pulJ - prepilin peptidase-dependent protein, at 3,890,274 to 3,890,837 pulJ OKFHMN_20030: ygdB - DUF2509 domain-containing protein, at 3,890,834 to 3,891,241 ygdB OKFHMN_20035: ppdC - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 3,891,226 to 3,891,549 ppdC OKFHMN_20040: recC - exodeoxyribonuclease V subunit gamma, at 3,891,562 to 3,894,930 recC Position (kb) 3891 3892Strain fitness (log2 ratio) -1 0 1 2at 3890.338 kb on + strand, within pulJat 3890.338 kb on + strand, within pulJat 3890.338 kb on + strand, within pulJat 3890.528 kb on + strand, within pulJat 3890.528 kb on + strand, within pulJat 3890.528 kb on + strand, within pulJat 3890.562 kb on - strand, within pulJat 3890.562 kb on - strand, within pulJat 3890.699 kb on + strand, within pulJat 3890.720 kb on - strand, within pulJat 3890.762 kb on + strand, within pulJat 3890.811 kb on + strandat 3890.811 kb on + strandat 3890.812 kb on - strandat 3890.901 kb on + strand, within ygdBat 3891.049 kb on - strand, within ygdBat 3891.108 kb on + strand, within ygdBat 3891.111 kb on + strand, within ygdBat 3891.111 kb on + strand, within ygdBat 3891.111 kb on + strand, within ygdBat 3891.112 kb on - strand, within ygdBat 3891.226 kb on - strandat 3891.336 kb on - strand, within ppdCat 3891.349 kb on - strand, within ppdCat 3891.982 kb on + strand, within recC

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction EV146
remove
3,890,338 + pulJ OKFHMN_20025 0.11 +0.5
3,890,338 + pulJ OKFHMN_20025 0.11 +0.4
3,890,338 + pulJ OKFHMN_20025 0.11 +0.3
3,890,528 + pulJ OKFHMN_20025 0.45 -0.5
3,890,528 + pulJ OKFHMN_20025 0.45 +0.0
3,890,528 + pulJ OKFHMN_20025 0.45 +0.2
3,890,562 - pulJ OKFHMN_20025 0.51 +0.2
3,890,562 - pulJ OKFHMN_20025 0.51 +1.0
3,890,699 + pulJ OKFHMN_20025 0.75 -0.1
3,890,720 - pulJ OKFHMN_20025 0.79 +0.4
3,890,762 + pulJ OKFHMN_20025 0.87 +0.4
3,890,811 + +1.0
3,890,811 + -0.3
3,890,812 - +1.1
3,890,901 + ygdB OKFHMN_20030 0.16 +0.2
3,891,049 - ygdB OKFHMN_20030 0.53 -0.3
3,891,108 + ygdB OKFHMN_20030 0.67 -0.3
3,891,111 + ygdB OKFHMN_20030 0.68 +0.1
3,891,111 + ygdB OKFHMN_20030 0.68 +0.8
3,891,111 + ygdB OKFHMN_20030 0.68 -0.8
3,891,112 - ygdB OKFHMN_20030 0.68 +0.7
3,891,226 - -0.8
3,891,336 - ppdC OKFHMN_20035 0.34 -0.0
3,891,349 - ppdC OKFHMN_20035 0.38 -0.1
3,891,982 + recC OKFHMN_20040 0.12 +2.0

Or see this region's nucleotide sequence