Strain Fitness in Escherichia coli ECRC100 around OKFHMN_19040

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybD and hybE overlap by 8 nucleotideshybE and hypA overlap by 8 nucleotideshypA and hybG are separated by 12 nucleotideshybG and yghU are separated by 122 nucleotides OKFHMN_19025: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 3,694,756 to 3,695,250 hybD OKFHMN_19030: hybE - hydrogenase-2 assembly chaperone, at 3,695,243 to 3,695,731 hybE OKFHMN_19035: hypA - hydrogenase maturation nickel metallochaperone HypA, at 3,695,724 to 3,696,065 hypA OKFHMN_19040: hybG - hydrogenase maturation factor HybG, at 3,696,078 to 3,696,326 hybG OKFHMN_19050: yghU - glutathione-dependent disulfide-bond oxidoreductase, at 3,696,449 to 3,697,315 yghU Position (kb) 3696 3697Strain fitness (log2 ratio) -2 -1 0 1at 3695.257 kb on - strandat 3695.289 kb on + strandat 3695.289 kb on + strandat 3695.555 kb on + strand, within hybEat 3695.556 kb on - strand, within hybEat 3695.908 kb on + strand, within hypAat 3695.909 kb on - strand, within hypAat 3695.912 kb on + strand, within hypAat 3695.912 kb on + strand, within hypAat 3695.919 kb on + strand, within hypAat 3695.921 kb on + strand, within hypAat 3695.922 kb on - strand, within hypAat 3696.074 kb on - strandat 3696.083 kb on + strandat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.149 kb on - strand, within hybGat 3696.194 kb on + strand, within hybGat 3696.194 kb on + strand, within hybGat 3696.283 kb on + strand, within hybGat 3696.283 kb on + strand, within hybGat 3696.315 kb on - strandat 3696.341 kb on - strandat 3696.463 kb on + strandat 3696.609 kb on + strand, within yghUat 3696.609 kb on + strand, within yghUat 3696.609 kb on + strand, within yghUat 3696.610 kb on - strand, within yghUat 3696.702 kb on + strand, within yghUat 3696.703 kb on - strand, within yghUat 3696.724 kb on + strand, within yghUat 3696.823 kb on - strand, within yghUat 3696.988 kb on + strand, within yghUat 3697.076 kb on + strand, within yghUat 3697.077 kb on - strand, within yghUat 3697.077 kb on - strand, within yghUat 3697.092 kb on + strand, within yghUat 3697.092 kb on + strand, within yghUat 3697.135 kb on + strand, within yghUat 3697.135 kb on + strand, within yghUat 3697.182 kb on - strand, within yghUat 3697.300 kb on - strandat 3697.303 kb on + strandat 3697.303 kb on + strandat 3697.304 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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3,695,257 - -0.5
3,695,289 + -0.2
3,695,289 + -0.6
3,695,555 + hybE OKFHMN_19030 0.64 -0.9
3,695,556 - hybE OKFHMN_19030 0.64 -1.7
3,695,908 + hypA OKFHMN_19035 0.54 +0.9
3,695,909 - hypA OKFHMN_19035 0.54 -1.3
3,695,912 + hypA OKFHMN_19035 0.55 -2.0
3,695,912 + hypA OKFHMN_19035 0.55 +0.4
3,695,919 + hypA OKFHMN_19035 0.57 -0.5
3,695,921 + hypA OKFHMN_19035 0.58 -0.4
3,695,922 - hypA OKFHMN_19035 0.58 +0.1
3,696,074 - -0.2
3,696,083 + +0.2
3,696,148 + hybG OKFHMN_19040 0.28 -0.3
3,696,148 + hybG OKFHMN_19040 0.28 +0.5
3,696,148 + hybG OKFHMN_19040 0.28 +0.5
3,696,148 + hybG OKFHMN_19040 0.28 -0.4
3,696,149 - hybG OKFHMN_19040 0.29 +0.6
3,696,194 + hybG OKFHMN_19040 0.47 +0.5
3,696,194 + hybG OKFHMN_19040 0.47 -0.3
3,696,283 + hybG OKFHMN_19040 0.82 +0.5
3,696,283 + hybG OKFHMN_19040 0.82 +0.6
3,696,315 - +1.2
3,696,341 - +1.0
3,696,463 + +0.2
3,696,609 + yghU OKFHMN_19050 0.18 -0.5
3,696,609 + yghU OKFHMN_19050 0.18 +0.5
3,696,609 + yghU OKFHMN_19050 0.18 -0.2
3,696,610 - yghU OKFHMN_19050 0.19 -0.8
3,696,702 + yghU OKFHMN_19050 0.29 +0.1
3,696,703 - yghU OKFHMN_19050 0.29 +0.1
3,696,724 + yghU OKFHMN_19050 0.32 -0.4
3,696,823 - yghU OKFHMN_19050 0.43 -0.0
3,696,988 + yghU OKFHMN_19050 0.62 -0.8
3,697,076 + yghU OKFHMN_19050 0.72 +0.0
3,697,077 - yghU OKFHMN_19050 0.72 -0.6
3,697,077 - yghU OKFHMN_19050 0.72 -1.8
3,697,092 + yghU OKFHMN_19050 0.74 +0.5
3,697,092 + yghU OKFHMN_19050 0.74 -0.2
3,697,135 + yghU OKFHMN_19050 0.79 -0.1
3,697,135 + yghU OKFHMN_19050 0.79 -0.7
3,697,182 - yghU OKFHMN_19050 0.85 -0.7
3,697,300 - -1.5
3,697,303 + -0.3
3,697,303 + -0.2
3,697,304 - -0.0

Or see this region's nucleotide sequence