Strain Fitness in Escherichia coli ECRC100 around OKFHMN_18655

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnfeF and Ile2_trna are separated by 53 nucleotidesIle2_trna and mug are separated by 124 nucleotidesmug and rpoD are separated by 78 nucleotidesrpoD and dnaG are separated by 194 nucleotides OKFHMN_18640: nfeF - NADPH-dependent ferric chelate reductase, at 3,620,373 to 3,621,137 nfeF OKFHMN_18645: Ile2_trna - tRNA-Ile2(cat), at 3,621,191 to 3,621,266 _trna OKFHMN_18650: mug - G/U mismatch-specific DNA glycosylase, at 3,621,391 to 3,621,897 mug OKFHMN_18655: rpoD - RNA polymerase sigma factor RpoD, at 3,621,976 to 3,623,817 rpoD OKFHMN_18660: dnaG - DNA primase, at 3,624,012 to 3,625,757 dnaG Position (kb) 3621 3622 3623 3624Strain fitness (log2 ratio) -3 -2 -1 0 1at 3620.980 kb on + strand, within nfeFat 3621.021 kb on + strand, within nfeFat 3621.021 kb on + strand, within nfeFat 3621.186 kb on - strandat 3621.186 kb on - strandat 3621.188 kb on - strandat 3621.258 kb on + strand, within Ile2_trnaat 3621.280 kb on - strandat 3621.287 kb on - strandat 3621.331 kb on + strandat 3621.359 kb on - strandat 3621.386 kb on + strandat 3621.386 kb on + strandat 3621.386 kb on + strandat 3621.387 kb on - strandat 3621.387 kb on - strandat 3621.387 kb on - strandat 3621.387 kb on - strandat 3621.403 kb on - strandat 3621.512 kb on + strand, within mugat 3621.512 kb on + strand, within mugat 3621.512 kb on + strand, within mugat 3621.512 kb on + strand, within mugat 3621.513 kb on - strand, within mugat 3621.513 kb on - strand, within mugat 3621.660 kb on + strand, within mugat 3621.724 kb on + strand, within mugat 3621.724 kb on + strand, within mugat 3621.978 kb on - strandat 3623.925 kb on - strandat 3623.939 kb on - strandat 3623.939 kb on - strandat 3623.939 kb on - strandat 3623.939 kb on - strandat 3623.939 kb on - strandat 3623.939 kb on - strandat 3623.943 kb on - strandat 3624.001 kb on - strandat 3624.001 kb on - strandat 3624.001 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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3,620,980 + nfeF OKFHMN_18640 0.79 -0.7
3,621,021 + nfeF OKFHMN_18640 0.85 +0.3
3,621,021 + nfeF OKFHMN_18640 0.85 +0.1
3,621,186 - -0.1
3,621,186 - -1.2
3,621,188 - +0.0
3,621,258 + Ile2_trna OKFHMN_18645 0.88 -0.9
3,621,280 - -3.3
3,621,287 - -2.3
3,621,331 + -0.5
3,621,359 - +0.4
3,621,386 + +0.4
3,621,386 + -1.1
3,621,386 + +0.2
3,621,387 - -1.7
3,621,387 - +0.1
3,621,387 - -0.5
3,621,387 - -1.3
3,621,403 - -0.1
3,621,512 + mug OKFHMN_18650 0.24 -1.4
3,621,512 + mug OKFHMN_18650 0.24 +0.4
3,621,512 + mug OKFHMN_18650 0.24 +0.2
3,621,512 + mug OKFHMN_18650 0.24 -0.2
3,621,513 - mug OKFHMN_18650 0.24 +0.4
3,621,513 - mug OKFHMN_18650 0.24 -1.6
3,621,660 + mug OKFHMN_18650 0.53 -0.8
3,621,724 + mug OKFHMN_18650 0.66 -0.3
3,621,724 + mug OKFHMN_18650 0.66 -0.6
3,621,978 - +0.4
3,623,925 - -0.3
3,623,939 - -0.6
3,623,939 - -0.6
3,623,939 - -1.8
3,623,939 - -1.0
3,623,939 - -1.2
3,623,939 - -1.3
3,623,943 - -0.8
3,624,001 - +0.5
3,624,001 - -1.0
3,624,001 - +1.1

Or see this region's nucleotide sequence