Strain Fitness in Escherichia coli ECRC100 around OKFHMN_17145

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpS and phoB are separated by 130 nucleotidesphoB and frlD are separated by 99 nucleotidesfrlD and frlD are separated by 18 nucleotidesfrlD and frlC overlap by 1 nucleotides OKFHMN_17140: trpS - tryptophan--tRNA ligase, at 3,349,247 to 3,350,251 trpS OKFHMN_17145: phoB - transcriptional regulator PhoB, at 3,350,382 to 3,351,113 phoB OKFHMN_17150: frlD - fructoselysine 6-kinase, at 3,351,213 to 3,351,647 frlD OKFHMN_17155: frlD - fructoselysine 6-kinase, at 3,351,666 to 3,351,998 frlD OKFHMN_17160: frlC - fructoselysine 3-epimerase, at 3,351,998 to 3,352,825 frlC Position (kb) 3350 3351 3352Strain fitness (log2 ratio) -1 0 1 2at 3350.249 kb on + strandat 3350.323 kb on - strandat 3350.407 kb on - strandat 3350.552 kb on + strand, within phoBat 3350.679 kb on - strand, within phoBat 3350.685 kb on - strand, within phoBat 3350.685 kb on - strand, within phoBat 3350.689 kb on - strand, within phoBat 3350.700 kb on - strand, within phoBat 3350.708 kb on - strand, within phoBat 3350.708 kb on - strand, within phoBat 3350.890 kb on - strand, within phoBat 3350.890 kb on - strand, within phoBat 3350.923 kb on - strand, within phoBat 3350.938 kb on - strand, within phoBat 3350.963 kb on + strand, within phoBat 3350.964 kb on - strand, within phoBat 3350.978 kb on + strand, within phoBat 3350.978 kb on + strand, within phoBat 3350.978 kb on + strand, within phoBat 3350.979 kb on - strand, within phoBat 3350.979 kb on - strand, within phoBat 3350.980 kb on + strand, within phoBat 3350.980 kb on + strand, within phoBat 3350.980 kb on + strand, within phoBat 3350.981 kb on - strand, within phoBat 3350.981 kb on - strand, within phoBat 3351.028 kb on + strand, within phoBat 3351.045 kb on - strandat 3351.105 kb on - strandat 3351.114 kb on - strandat 3351.114 kb on - strandat 3351.129 kb on - strandat 3351.140 kb on + strandat 3351.149 kb on + strandat 3351.152 kb on - strandat 3351.171 kb on + strandat 3351.172 kb on - strandat 3351.230 kb on - strandat 3351.236 kb on + strandat 3351.237 kb on - strandat 3351.237 kb on - strandat 3351.655 kb on + strandat 3351.667 kb on + strandat 3351.674 kb on - strandat 3351.674 kb on - strandat 3351.674 kb on - strandat 3351.867 kb on - strand, within frlDat 3351.883 kb on - strand, within frlDat 3351.941 kb on + strand, within frlDat 3352.070 kb on - strandat 3352.071 kb on + strandat 3352.071 kb on + strandat 3352.071 kb on + strandat 3352.071 kb on + strandat 3352.072 kb on - strandat 3352.072 kb on - strandat 3352.072 kb on - strandat 3352.080 kb on - strandat 3352.108 kb on - strand, within frlC

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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3,350,249 + +0.4
3,350,323 - -0.7
3,350,407 - +1.4
3,350,552 + phoB OKFHMN_17145 0.23 +0.5
3,350,679 - phoB OKFHMN_17145 0.41 -1.1
3,350,685 - phoB OKFHMN_17145 0.41 +0.3
3,350,685 - phoB OKFHMN_17145 0.41 -1.0
3,350,689 - phoB OKFHMN_17145 0.42 -0.6
3,350,700 - phoB OKFHMN_17145 0.43 +0.1
3,350,708 - phoB OKFHMN_17145 0.45 +0.2
3,350,708 - phoB OKFHMN_17145 0.45 +0.5
3,350,890 - phoB OKFHMN_17145 0.69 +0.1
3,350,890 - phoB OKFHMN_17145 0.69 -1.2
3,350,923 - phoB OKFHMN_17145 0.74 +0.8
3,350,938 - phoB OKFHMN_17145 0.76 -0.0
3,350,963 + phoB OKFHMN_17145 0.79 -0.2
3,350,964 - phoB OKFHMN_17145 0.80 -0.6
3,350,978 + phoB OKFHMN_17145 0.81 +0.5
3,350,978 + phoB OKFHMN_17145 0.81 +1.1
3,350,978 + phoB OKFHMN_17145 0.81 -1.0
3,350,979 - phoB OKFHMN_17145 0.82 -0.3
3,350,979 - phoB OKFHMN_17145 0.82 -0.7
3,350,980 + phoB OKFHMN_17145 0.82 -0.4
3,350,980 + phoB OKFHMN_17145 0.82 +0.1
3,350,980 + phoB OKFHMN_17145 0.82 -0.0
3,350,981 - phoB OKFHMN_17145 0.82 -0.5
3,350,981 - phoB OKFHMN_17145 0.82 +0.5
3,351,028 + phoB OKFHMN_17145 0.88 -0.1
3,351,045 - +0.1
3,351,105 - +1.1
3,351,114 - +0.0
3,351,114 - -0.6
3,351,129 - -0.8
3,351,140 + -0.0
3,351,149 + +0.0
3,351,152 - -0.1
3,351,171 + +0.3
3,351,172 - +0.6
3,351,230 - -1.0
3,351,236 + -0.8
3,351,237 - +1.3
3,351,237 - -1.0
3,351,655 + +0.4
3,351,667 + -1.2
3,351,674 - -0.2
3,351,674 - -1.2
3,351,674 - -0.5
3,351,867 - frlD OKFHMN_17155 0.60 +0.2
3,351,883 - frlD OKFHMN_17155 0.65 +0.2
3,351,941 + frlD OKFHMN_17155 0.83 +0.3
3,352,070 - +0.5
3,352,071 + +2.4
3,352,071 + +0.7
3,352,071 + -0.8
3,352,071 + -0.9
3,352,072 - +0.2
3,352,072 - +1.1
3,352,072 - -0.9
3,352,080 - -0.3
3,352,108 - frlC OKFHMN_17160 0.13 +0.8

Or see this region's nucleotide sequence