Strain Fitness in Escherichia coli ECRC100 around OKFHMN_12820

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnrfC and nrfB overlap by 4 nucleotidesnrfB and nrfA are separated by 44 nucleotidesnrfA and ytcB are separated by 96 nucleotidesytcB and acs are separated by 239 nucleotides OKFHMN_12810: nrfC - cytochrome c nitrite reductase Fe-S protein, at 2,458,008 to 2,458,679 nrfC OKFHMN_12815: nrfB - cytochrome c nitrite reductase pentaheme subunit, at 2,458,676 to 2,459,242 nrfB OKFHMN_12820: nrfA - ammonia-forming nitrite reductase cytochrome c552 subunit, at 2,459,287 to 2,460,723 nrfA OKFHMN_12825: ytcB - Protein YtcB, at 2,460,820 to 2,460,876 ytcB OKFHMN_12830: acs - acetate--CoA ligase, at 2,461,116 to 2,463,074 acs Position (kb) 2459 2460 2461Strain fitness (log2 ratio) -2 -1 0 1 2at 2458.524 kb on - strand, within nrfCat 2458.524 kb on - strand, within nrfCat 2458.714 kb on + strandat 2458.781 kb on + strand, within nrfBat 2458.781 kb on + strand, within nrfBat 2458.903 kb on + strand, within nrfBat 2458.903 kb on + strand, within nrfBat 2458.903 kb on + strand, within nrfBat 2458.903 kb on + strand, within nrfBat 2458.904 kb on - strand, within nrfBat 2458.932 kb on + strand, within nrfBat 2458.933 kb on - strand, within nrfBat 2459.065 kb on + strand, within nrfBat 2459.065 kb on + strand, within nrfBat 2459.065 kb on + strand, within nrfBat 2459.065 kb on + strand, within nrfBat 2459.066 kb on - strand, within nrfBat 2459.066 kb on - strand, within nrfBat 2459.066 kb on - strand, within nrfBat 2459.134 kb on - strand, within nrfBat 2459.170 kb on + strand, within nrfBat 2459.220 kb on - strandat 2459.232 kb on - strandat 2459.728 kb on - strand, within nrfAat 2459.770 kb on - strand, within nrfAat 2459.812 kb on - strand, within nrfAat 2459.924 kb on + strand, within nrfAat 2459.970 kb on + strand, within nrfAat 2459.971 kb on - strand, within nrfAat 2460.002 kb on + strand, within nrfAat 2460.002 kb on + strand, within nrfAat 2460.002 kb on + strand, within nrfAat 2460.002 kb on + strand, within nrfAat 2460.049 kb on - strand, within nrfAat 2460.077 kb on + strand, within nrfAat 2460.174 kb on - strand, within nrfAat 2460.190 kb on + strand, within nrfAat 2460.191 kb on - strand, within nrfAat 2460.232 kb on - strand, within nrfAat 2460.250 kb on + strand, within nrfAat 2460.250 kb on + strand, within nrfAat 2460.251 kb on - strand, within nrfAat 2460.300 kb on - strand, within nrfAat 2460.348 kb on + strand, within nrfAat 2460.462 kb on - strand, within nrfAat 2460.567 kb on - strand, within nrfAat 2460.567 kb on - strand, within nrfAat 2460.706 kb on + strandat 2460.723 kb on + strandat 2460.795 kb on + strandat 2460.795 kb on + strandat 2460.795 kb on + strandat 2460.796 kb on - strandat 2460.796 kb on - strandat 2460.796 kb on - strandat 2460.822 kb on - strandat 2460.822 kb on - strandat 2460.869 kb on - strand, within ytcBat 2461.198 kb on - strandat 2461.224 kb on - strandat 2461.316 kb on + strand, within acsat 2461.317 kb on - strand, within acsat 2461.317 kb on - strand, within acsat 2461.436 kb on + strand, within acsat 2461.436 kb on + strand, within acsat 2461.437 kb on - strand, within acsat 2461.437 kb on - strand, within acsat 2461.437 kb on - strand, within acsat 2461.443 kb on + strand, within acsat 2461.443 kb on + strand, within acsat 2461.445 kb on + strand, within acsat 2461.482 kb on - strand, within acsat 2461.527 kb on + strand, within acsat 2461.528 kb on - strand, within acsat 2461.698 kb on - strand, within acs

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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2,458,524 - nrfC OKFHMN_12810 0.77 -0.9
2,458,524 - nrfC OKFHMN_12810 0.77 +0.1
2,458,714 + -0.0
2,458,781 + nrfB OKFHMN_12815 0.19 -0.1
2,458,781 + nrfB OKFHMN_12815 0.19 -0.3
2,458,903 + nrfB OKFHMN_12815 0.40 +0.3
2,458,903 + nrfB OKFHMN_12815 0.40 +0.3
2,458,903 + nrfB OKFHMN_12815 0.40 +0.3
2,458,903 + nrfB OKFHMN_12815 0.40 +0.4
2,458,904 - nrfB OKFHMN_12815 0.40 -1.2
2,458,932 + nrfB OKFHMN_12815 0.45 -0.3
2,458,933 - nrfB OKFHMN_12815 0.45 +0.0
2,459,065 + nrfB OKFHMN_12815 0.69 +1.3
2,459,065 + nrfB OKFHMN_12815 0.69 -0.2
2,459,065 + nrfB OKFHMN_12815 0.69 +0.0
2,459,065 + nrfB OKFHMN_12815 0.69 +1.2
2,459,066 - nrfB OKFHMN_12815 0.69 +0.1
2,459,066 - nrfB OKFHMN_12815 0.69 -0.7
2,459,066 - nrfB OKFHMN_12815 0.69 -0.3
2,459,134 - nrfB OKFHMN_12815 0.81 +1.4
2,459,170 + nrfB OKFHMN_12815 0.87 +0.7
2,459,220 - -0.9
2,459,232 - +0.3
2,459,728 - nrfA OKFHMN_12820 0.31 -0.0
2,459,770 - nrfA OKFHMN_12820 0.34 -0.2
2,459,812 - nrfA OKFHMN_12820 0.37 +0.2
2,459,924 + nrfA OKFHMN_12820 0.44 +1.1
2,459,970 + nrfA OKFHMN_12820 0.48 +0.7
2,459,971 - nrfA OKFHMN_12820 0.48 +0.6
2,460,002 + nrfA OKFHMN_12820 0.50 +0.6
2,460,002 + nrfA OKFHMN_12820 0.50 -0.2
2,460,002 + nrfA OKFHMN_12820 0.50 -0.5
2,460,002 + nrfA OKFHMN_12820 0.50 +0.0
2,460,049 - nrfA OKFHMN_12820 0.53 -0.1
2,460,077 + nrfA OKFHMN_12820 0.55 +0.0
2,460,174 - nrfA OKFHMN_12820 0.62 +1.3
2,460,190 + nrfA OKFHMN_12820 0.63 -0.2
2,460,191 - nrfA OKFHMN_12820 0.63 +0.5
2,460,232 - nrfA OKFHMN_12820 0.66 +0.8
2,460,250 + nrfA OKFHMN_12820 0.67 +0.5
2,460,250 + nrfA OKFHMN_12820 0.67 -0.1
2,460,251 - nrfA OKFHMN_12820 0.67 +0.2
2,460,300 - nrfA OKFHMN_12820 0.70 +0.9
2,460,348 + nrfA OKFHMN_12820 0.74 -0.1
2,460,462 - nrfA OKFHMN_12820 0.82 +1.3
2,460,567 - nrfA OKFHMN_12820 0.89 -0.1
2,460,567 - nrfA OKFHMN_12820 0.89 +0.9
2,460,706 + +1.8
2,460,723 + -0.1
2,460,795 + +1.5
2,460,795 + +1.1
2,460,795 + +1.1
2,460,796 - -0.3
2,460,796 - -0.4
2,460,796 - +0.5
2,460,822 - -0.3
2,460,822 - +1.1
2,460,869 - ytcB OKFHMN_12825 0.86 -0.3
2,461,198 - +0.1
2,461,224 - +0.1
2,461,316 + acs OKFHMN_12830 0.10 -0.6
2,461,317 - acs OKFHMN_12830 0.10 +0.2
2,461,317 - acs OKFHMN_12830 0.10 +1.7
2,461,436 + acs OKFHMN_12830 0.16 +0.1
2,461,436 + acs OKFHMN_12830 0.16 -0.2
2,461,437 - acs OKFHMN_12830 0.16 -0.5
2,461,437 - acs OKFHMN_12830 0.16 +0.5
2,461,437 - acs OKFHMN_12830 0.16 +1.0
2,461,443 + acs OKFHMN_12830 0.17 -0.1
2,461,443 + acs OKFHMN_12830 0.17 -0.7
2,461,445 + acs OKFHMN_12830 0.17 -1.9
2,461,482 - acs OKFHMN_12830 0.19 -0.3
2,461,527 + acs OKFHMN_12830 0.21 +0.3
2,461,528 - acs OKFHMN_12830 0.21 +0.8
2,461,698 - acs OKFHMN_12830 0.30 -0.2

Or see this region's nucleotide sequence