Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_05615 and OKFHMN_05620 overlap by 4 nucleotides OKFHMN_05620 and OKFHMN_05625 are separated by 46 nucleotides OKFHMN_05625 and OKFHMN_05630 are separated by 49 nucleotides OKFHMN_05630 and OKFHMN_05635 overlap by 1 nucleotides OKFHMN_05635 and yeeS are separated by 68 nucleotides yeeS and OKFHMN_05645 are separated by 15 nucleotides
OKFHMN_05615: OKFHMN_05615 - hypothetical protein, at 960,493 to 960,981
_05615
OKFHMN_05620: OKFHMN_05620 - toxin, at 960,978 to 961,355
_05620
OKFHMN_05625: OKFHMN_05625 - antitoxin, at 961,402 to 961,776
_05625
OKFHMN_05630: OKFHMN_05630 - Phage-related protein, at 961,826 to 961,939
_05630
OKFHMN_05635: OKFHMN_05635 - DUF987 domain-containing protein, at 961,939 to 962,160
_05635
OKFHMN_05640: yeeS - UPF0758 protein YeeS, at 962,229 to 962,705
yeeS
OKFHMN_05645: OKFHMN_05645 - restriction endonuclease, at 962,721 to 963,206
_05645
Position (kb)
961
962 Strain fitness (log2 ratio)
-1
0
1
2 at 961.148 kb on - strand, within OKFHMN_05620 at 961.860 kb on + strand, within OKFHMN_05630 at 961.860 kb on + strand, within OKFHMN_05630 at 961.860 kb on + strand, within OKFHMN_05630 at 961.860 kb on + strand, within OKFHMN_05630 at 962.080 kb on - strand, within OKFHMN_05635 at 962.168 kb on + strand at 962.187 kb on + strand at 962.187 kb on + strand at 962.337 kb on - strand, within yeeS at 962.806 kb on + strand, within OKFHMN_05645
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 961,148 - OKFHMN_05620 0.45 -0.5 961,860 + OKFHMN_05630 0.30 -0.8 961,860 + OKFHMN_05630 0.30 -0.3 961,860 + OKFHMN_05630 0.30 -0.5 961,860 + OKFHMN_05630 0.30 +0.8 962,080 - OKFHMN_05635 0.64 -1.6 962,168 + +0.2 962,187 + -0.8 962,187 + +0.6 962,337 - yeeS OKFHMN_05640 0.23 -1.4 962,806 + OKFHMN_05645 0.17 +2.3
Or see this region's nucleotide sequence