Strain Fitness in Escherichia coli ECRC100 around OKFHMN_04735

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcspG and OKFHMN_04735 are separated by 939 nucleotidesOKFHMN_04735 and gfcB are separated by 106 nucleotidesgfcB and gfcC overlap by 4 nucleotides OKFHMN_04730: cspG - cold shock protein CspG, at 785,999 to 786,211 cspG OKFHMN_04735: OKFHMN_04735 - Threonine-rich inner membrane protein GfcA, at 787,151 to 787,456 _04735 OKFHMN_04740: gfcB - Uncharacterized lipoprotein GfcB, at 787,563 to 788,207 gfcB OKFHMN_04745: gfcC - Uncharacterized protein GfcC, at 788,204 to 788,950 gfcC Position (kb) 787 788Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 786.271 kb on - strandat 786.271 kb on - strandat 786.337 kb on - strandat 786.633 kb on + strandat 786.727 kb on - strandat 786.836 kb on - strandat 786.855 kb on + strandat 786.898 kb on + strandat 786.990 kb on + strandat 787.041 kb on + strandat 787.094 kb on + strandat 787.094 kb on + strandat 787.116 kb on + strandat 787.120 kb on + strandat 787.252 kb on + strand, within OKFHMN_04735at 787.255 kb on + strand, within OKFHMN_04735at 787.300 kb on + strand, within OKFHMN_04735at 787.386 kb on + strand, within OKFHMN_04735at 787.505 kb on + strandat 787.768 kb on + strand, within gfcBat 788.048 kb on + strand, within gfcBat 788.168 kb on + strandat 788.209 kb on + strandat 788.214 kb on + strandat 788.222 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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786,271 - +0.4
786,271 - -1.8
786,337 - +0.7
786,633 + +0.7
786,727 - +0.6
786,836 - -0.2
786,855 + -0.3
786,898 + +0.2
786,990 + -2.1
787,041 + -1.3
787,094 + -1.9
787,094 + -4.4
787,116 + -2.4
787,120 + -1.1
787,252 + OKFHMN_04735 0.33 -1.5
787,255 + OKFHMN_04735 0.34 -2.4
787,300 + OKFHMN_04735 0.49 -1.9
787,386 + OKFHMN_04735 0.77 -1.7
787,505 + -1.9
787,768 + gfcB OKFHMN_04740 0.32 +0.2
788,048 + gfcB OKFHMN_04740 0.75 -2.8
788,168 + -2.7
788,209 + -2.0
788,214 + -2.5
788,222 + -2.3

Or see this region's nucleotide sequence