Experiment: EV146
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_03300 and OKFHMN_03305 overlap by 4 nucleotides OKFHMN_03305 and OKFHMN_03310 are separated by 453 nucleotides OKFHMN_03310 and rusA overlap by 4 nucleotides rusA and OKFHMN_03320 are separated by 12 nucleotides
OKFHMN_03300: OKFHMN_03300 - TciB/TerA-like protein, at 521,321 to 521,488
_03300
OKFHMN_03305: OKFHMN_03305 - TciA/TerB-like protein, at 521,485 to 521,631
_03305
OKFHMN_03310: OKFHMN_03310 - antitermination protein, at 522,085 to 522,906
_03310
OKFHMN_03315: rusA - Endodeoxyribonuclease RusA, at 522,903 to 523,277
rusA
OKFHMN_03320: OKFHMN_03320 - DUF968 domain-containing protein, at 523,290 to 524,339
_03320
Position (kb)
522
523 Strain fitness (log2 ratio)
-1
0
1
2 at 521.896 kb on - strand at 522.186 kb on + strand, within OKFHMN_03310 at 522.187 kb on - strand, within OKFHMN_03310 at 522.226 kb on - strand, within OKFHMN_03310 at 522.298 kb on - strand, within OKFHMN_03310 at 522.308 kb on - strand, within OKFHMN_03310 at 522.407 kb on - strand, within OKFHMN_03310 at 522.616 kb on - strand, within OKFHMN_03310 at 522.616 kb on - strand, within OKFHMN_03310 at 522.616 kb on - strand, within OKFHMN_03310
Per-strain Table
Position Strand Gene LocusTag Fraction EV146 remove 521,896 - +1.1 522,186 + OKFHMN_03310 0.12 -0.1 522,187 - OKFHMN_03310 0.12 +2.7 522,226 - OKFHMN_03310 0.17 -1.1 522,298 - OKFHMN_03310 0.26 +0.7 522,308 - OKFHMN_03310 0.27 -0.4 522,407 - OKFHMN_03310 0.39 +0.9 522,616 - OKFHMN_03310 0.65 +0.1 522,616 - OKFHMN_03310 0.65 -1.4 522,616 - OKFHMN_03310 0.65 +0.6
Or see this region's nucleotide sequence