Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01770

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpdxI and OKFHMN_01770 are separated by 219 nucleotidesOKFHMN_01770 and OKFHMN_01775 are separated by 219 nucleotides OKFHMN_01765: pdxI - pyridoxine 4-dehydrogenase, at 260,153 to 261,013 pdxI OKFHMN_01770: OKFHMN_01770 - BigB-like protein, at 261,233 to 263,692 _01770 OKFHMN_01775: OKFHMN_01775 - surface-exposed virulence protein BigA, at 263,912 to 266,791 _01775 Position (kb) 261 262 263 264Strain fitness (log2 ratio) -1 0 1 2at 260.539 kb on + strand, within pdxIat 261.110 kb on + strandat 261.334 kb on - strandat 261.448 kb on - strandat 261.812 kb on - strand, within OKFHMN_01770at 261.968 kb on - strand, within OKFHMN_01770at 261.968 kb on - strand, within OKFHMN_01770at 262.201 kb on - strand, within OKFHMN_01770at 262.525 kb on + strand, within OKFHMN_01770at 262.528 kb on - strand, within OKFHMN_01770at 262.600 kb on - strand, within OKFHMN_01770at 263.205 kb on - strand, within OKFHMN_01770at 263.237 kb on - strand, within OKFHMN_01770at 263.240 kb on + strand, within OKFHMN_01770at 263.621 kb on - strandat 263.846 kb on + strandat 263.868 kb on - strandat 263.898 kb on + strandat 264.224 kb on + strand, within OKFHMN_01775at 264.299 kb on - strand, within OKFHMN_01775at 264.389 kb on + strand, within OKFHMN_01775at 264.415 kb on - strand, within OKFHMN_01775at 264.428 kb on - strand, within OKFHMN_01775at 264.472 kb on + strand, within OKFHMN_01775at 264.497 kb on - strand, within OKFHMN_01775

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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260,539 + pdxI OKFHMN_01765 0.45 -0.6
261,110 + -0.0
261,334 - +0.1
261,448 - +1.1
261,812 - OKFHMN_01770 0.24 +1.1
261,968 - OKFHMN_01770 0.30 +0.7
261,968 - OKFHMN_01770 0.30 +1.2
262,201 - OKFHMN_01770 0.39 +0.2
262,525 + OKFHMN_01770 0.53 +0.7
262,528 - OKFHMN_01770 0.53 +0.6
262,600 - OKFHMN_01770 0.56 +1.0
263,205 - OKFHMN_01770 0.80 +1.3
263,237 - OKFHMN_01770 0.81 -0.7
263,240 + OKFHMN_01770 0.82 +1.1
263,621 - +0.7
263,846 + +0.1
263,868 - -0.9
263,898 + +2.1
264,224 + OKFHMN_01775 0.11 -0.3
264,299 - OKFHMN_01775 0.13 +0.7
264,389 + OKFHMN_01775 0.17 -0.1
264,415 - OKFHMN_01775 0.17 +0.3
264,428 - OKFHMN_01775 0.18 +1.3
264,472 + OKFHMN_01775 0.19 +0.8
264,497 - OKFHMN_01775 0.20 +0.1

Or see this region's nucleotide sequence