Strain Fitness in Escherichia coli ECRC100 around OKFHMN_01290

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgadB and gadC are separated by 155 nucleotidesgadC and digH are separated by 130 nucleotides OKFHMN_01285: gadB - glutamate decarboxylase, at 157,766 to 159,166 gadB OKFHMN_01290: gadC - acid resistance gamma-aminobutyrate antiporter GadC, at 159,322 to 160,857 gadC OKFHMN_01295: digH - Glycosyl hydrolase DigH, at 160,988 to 162,307 digH Position (kb) 159 160 161Strain fitness (log2 ratio) -1 0 1at 158.748 kb on - strand, within gadBat 159.252 kb on + strandat 160.828 kb on + strandat 160.830 kb on + strandat 160.856 kb on - strandat 160.859 kb on + strandat 160.873 kb on + strandat 160.875 kb on + strandat 160.875 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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158,748 - gadB OKFHMN_01285 0.70 +0.4
159,252 + -0.2
160,828 + +0.5
160,830 + -1.2
160,856 - +1.7
160,859 + -0.3
160,873 + +0.7
160,875 + +0.7
160,875 + +1.1

Or see this region's nucleotide sequence