Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00275

Experiment: EV146

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgspH and gspG overlap by 4 nucleotidesgspG and gspF are separated by 30 nucleotidesgspF and gspE are separated by 1 nucleotides OKFHMN_00265: gspH - type II secretion system minor pseudopilin GspH, at 53,998 to 54,552 gspH OKFHMN_00270: gspG - type II secretion system major pseudopilin GspG, at 54,549 to 54,983 gspG OKFHMN_00275: gspF - type II secretion system inner membrane protein GspF, at 55,014 to 56,237 gspF OKFHMN_00280: gspE - type II secretion system ATPase GspE, at 56,239 to 57,744 gspE Position (kb) 55 56 57Strain fitness (log2 ratio) -2 -1 0 1 2at 54.185 kb on + strand, within gspHat 54.263 kb on + strand, within gspHat 54.263 kb on + strand, within gspHat 54.264 kb on - strand, within gspHat 54.264 kb on - strand, within gspHat 54.264 kb on - strand, within gspHat 54.277 kb on - strand, within gspHat 54.293 kb on - strand, within gspHat 54.293 kb on - strand, within gspHat 54.333 kb on + strand, within gspHat 54.393 kb on + strand, within gspHat 54.393 kb on + strand, within gspHat 54.393 kb on + strand, within gspHat 54.394 kb on - strand, within gspHat 54.404 kb on + strand, within gspHat 54.405 kb on - strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.475 kb on + strand, within gspHat 54.476 kb on - strand, within gspHat 54.476 kb on - strand, within gspHat 54.476 kb on - strand, within gspHat 54.479 kb on - strand, within gspHat 54.585 kb on - strandat 54.629 kb on + strand, within gspGat 54.644 kb on - strand, within gspGat 54.644 kb on - strand, within gspGat 54.658 kb on - strand, within gspGat 54.667 kb on + strand, within gspGat 54.668 kb on - strand, within gspGat 54.704 kb on + strand, within gspGat 54.704 kb on + strand, within gspGat 54.721 kb on + strand, within gspGat 54.801 kb on - strand, within gspGat 54.811 kb on + strand, within gspGat 54.811 kb on + strand, within gspGat 54.812 kb on - strand, within gspGat 54.878 kb on + strand, within gspGat 54.878 kb on + strand, within gspGat 54.879 kb on - strand, within gspGat 54.933 kb on + strand, within gspGat 54.933 kb on + strand, within gspGat 54.943 kb on - strandat 54.956 kb on + strandat 54.956 kb on + strandat 54.957 kb on - strandat 54.957 kb on - strandat 55.003 kb on + strandat 55.004 kb on - strandat 55.019 kb on + strandat 55.020 kb on - strandat 55.020 kb on - strandat 55.071 kb on - strandat 55.235 kb on + strand, within gspFat 55.307 kb on + strand, within gspFat 55.311 kb on + strand, within gspFat 55.391 kb on + strand, within gspFat 55.392 kb on - strand, within gspFat 55.414 kb on + strand, within gspFat 55.414 kb on + strand, within gspFat 55.415 kb on - strand, within gspFat 55.415 kb on - strand, within gspFat 55.506 kb on + strand, within gspFat 55.506 kb on + strand, within gspFat 55.506 kb on + strand, within gspFat 55.507 kb on - strand, within gspFat 55.509 kb on + strand, within gspFat 55.509 kb on + strand, within gspFat 55.548 kb on + strand, within gspFat 55.549 kb on - strand, within gspFat 55.604 kb on + strand, within gspFat 55.604 kb on + strand, within gspFat 55.608 kb on + strand, within gspFat 55.629 kb on - strand, within gspFat 55.667 kb on + strand, within gspFat 55.668 kb on - strand, within gspFat 55.668 kb on - strand, within gspFat 55.717 kb on + strand, within gspFat 55.768 kb on + strand, within gspFat 55.768 kb on + strand, within gspFat 55.768 kb on + strand, within gspFat 55.923 kb on + strand, within gspFat 56.126 kb on + strandat 56.222 kb on - strandat 56.237 kb on + strandat 56.306 kb on - strandat 56.342 kb on + strandat 56.343 kb on - strandat 56.368 kb on - strandat 56.390 kb on + strand, within gspEat 56.391 kb on - strand, within gspEat 56.454 kb on - strand, within gspEat 56.454 kb on - strand, within gspEat 56.530 kb on + strand, within gspEat 56.531 kb on - strand, within gspEat 56.608 kb on + strand, within gspEat 56.676 kb on + strand, within gspEat 56.677 kb on - strand, within gspEat 56.682 kb on + strand, within gspEat 56.682 kb on + strand, within gspEat 56.683 kb on - strand, within gspEat 56.683 kb on - strand, within gspEat 56.683 kb on - strand, within gspEat 56.683 kb on - strand, within gspEat 56.683 kb on - strand, within gspEat 56.683 kb on - strand, within gspEat 56.686 kb on + strand, within gspEat 56.687 kb on - strand, within gspEat 56.687 kb on - strand, within gspEat 56.791 kb on + strand, within gspEat 56.871 kb on - strand, within gspEat 56.895 kb on + strand, within gspEat 56.895 kb on + strand, within gspEat 56.895 kb on + strand, within gspEat 56.896 kb on - strand, within gspEat 56.896 kb on - strand, within gspEat 56.926 kb on + strand, within gspEat 56.936 kb on + strand, within gspEat 56.936 kb on + strand, within gspEat 56.936 kb on + strand, within gspEat 57.069 kb on + strand, within gspEat 57.070 kb on - strand, within gspEat 57.079 kb on + strand, within gspEat 57.082 kb on + strand, within gspEat 57.083 kb on - strand, within gspEat 57.157 kb on - strand, within gspEat 57.222 kb on + strand, within gspEat 57.222 kb on + strand, within gspE

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Per-strain Table

Position Strand Gene LocusTag Fraction EV146
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54,185 + gspH OKFHMN_00265 0.34 -0.0
54,263 + gspH OKFHMN_00265 0.48 +0.5
54,263 + gspH OKFHMN_00265 0.48 +0.3
54,264 - gspH OKFHMN_00265 0.48 -0.3
54,264 - gspH OKFHMN_00265 0.48 -1.0
54,264 - gspH OKFHMN_00265 0.48 +1.0
54,277 - gspH OKFHMN_00265 0.50 -0.9
54,293 - gspH OKFHMN_00265 0.53 -0.3
54,293 - gspH OKFHMN_00265 0.53 -0.3
54,333 + gspH OKFHMN_00265 0.60 +0.2
54,393 + gspH OKFHMN_00265 0.71 +1.0
54,393 + gspH OKFHMN_00265 0.71 -0.6
54,393 + gspH OKFHMN_00265 0.71 -0.2
54,394 - gspH OKFHMN_00265 0.71 +0.7
54,404 + gspH OKFHMN_00265 0.73 +0.1
54,405 - gspH OKFHMN_00265 0.73 +0.1
54,475 + gspH OKFHMN_00265 0.86 +0.7
54,475 + gspH OKFHMN_00265 0.86 +0.9
54,475 + gspH OKFHMN_00265 0.86 +0.7
54,475 + gspH OKFHMN_00265 0.86 -0.8
54,475 + gspH OKFHMN_00265 0.86 -0.1
54,475 + gspH OKFHMN_00265 0.86 -0.2
54,476 - gspH OKFHMN_00265 0.86 +0.3
54,476 - gspH OKFHMN_00265 0.86 +0.3
54,476 - gspH OKFHMN_00265 0.86 +0.2
54,479 - gspH OKFHMN_00265 0.87 -0.3
54,585 - +0.4
54,629 + gspG OKFHMN_00270 0.18 -0.7
54,644 - gspG OKFHMN_00270 0.22 +0.3
54,644 - gspG OKFHMN_00270 0.22 +0.4
54,658 - gspG OKFHMN_00270 0.25 +0.4
54,667 + gspG OKFHMN_00270 0.27 -0.9
54,668 - gspG OKFHMN_00270 0.27 -1.6
54,704 + gspG OKFHMN_00270 0.36 -0.6
54,704 + gspG OKFHMN_00270 0.36 +0.9
54,721 + gspG OKFHMN_00270 0.40 +1.4
54,801 - gspG OKFHMN_00270 0.58 -0.4
54,811 + gspG OKFHMN_00270 0.60 +0.4
54,811 + gspG OKFHMN_00270 0.60 -0.7
54,812 - gspG OKFHMN_00270 0.60 -0.2
54,878 + gspG OKFHMN_00270 0.76 +1.2
54,878 + gspG OKFHMN_00270 0.76 -2.0
54,879 - gspG OKFHMN_00270 0.76 -0.6
54,933 + gspG OKFHMN_00270 0.88 -0.9
54,933 + gspG OKFHMN_00270 0.88 +1.7
54,943 - +0.8
54,956 + -0.1
54,956 + +0.2
54,957 - +0.8
54,957 - +0.0
55,003 + -2.3
55,004 - -0.3
55,019 + +0.3
55,020 - +0.0
55,020 - -0.6
55,071 - -1.0
55,235 + gspF OKFHMN_00275 0.18 +0.7
55,307 + gspF OKFHMN_00275 0.24 -0.7
55,311 + gspF OKFHMN_00275 0.24 +0.5
55,391 + gspF OKFHMN_00275 0.31 -0.3
55,392 - gspF OKFHMN_00275 0.31 +0.2
55,414 + gspF OKFHMN_00275 0.33 +0.1
55,414 + gspF OKFHMN_00275 0.33 +0.3
55,415 - gspF OKFHMN_00275 0.33 +1.0
55,415 - gspF OKFHMN_00275 0.33 -0.3
55,506 + gspF OKFHMN_00275 0.40 +2.4
55,506 + gspF OKFHMN_00275 0.40 +1.4
55,506 + gspF OKFHMN_00275 0.40 +1.0
55,507 - gspF OKFHMN_00275 0.40 +0.9
55,509 + gspF OKFHMN_00275 0.40 +0.3
55,509 + gspF OKFHMN_00275 0.40 -0.2
55,548 + gspF OKFHMN_00275 0.44 -0.1
55,549 - gspF OKFHMN_00275 0.44 +1.3
55,604 + gspF OKFHMN_00275 0.48 +0.7
55,604 + gspF OKFHMN_00275 0.48 -1.0
55,608 + gspF OKFHMN_00275 0.49 +1.3
55,629 - gspF OKFHMN_00275 0.50 +0.2
55,667 + gspF OKFHMN_00275 0.53 -1.6
55,668 - gspF OKFHMN_00275 0.53 -0.7
55,668 - gspF OKFHMN_00275 0.53 +2.0
55,717 + gspF OKFHMN_00275 0.57 +1.6
55,768 + gspF OKFHMN_00275 0.62 -0.1
55,768 + gspF OKFHMN_00275 0.62 +0.0
55,768 + gspF OKFHMN_00275 0.62 -0.3
55,923 + gspF OKFHMN_00275 0.74 -0.6
56,126 + +1.0
56,222 - -1.0
56,237 + +0.3
56,306 - -0.1
56,342 + +1.1
56,343 - +0.1
56,368 - -0.7
56,390 + gspE OKFHMN_00280 0.10 +0.0
56,391 - gspE OKFHMN_00280 0.10 +0.6
56,454 - gspE OKFHMN_00280 0.14 +0.8
56,454 - gspE OKFHMN_00280 0.14 +0.1
56,530 + gspE OKFHMN_00280 0.19 +0.3
56,531 - gspE OKFHMN_00280 0.19 +0.7
56,608 + gspE OKFHMN_00280 0.25 -0.4
56,676 + gspE OKFHMN_00280 0.29 +1.0
56,677 - gspE OKFHMN_00280 0.29 -0.8
56,682 + gspE OKFHMN_00280 0.29 +0.2
56,682 + gspE OKFHMN_00280 0.29 +1.4
56,683 - gspE OKFHMN_00280 0.29 -0.1
56,683 - gspE OKFHMN_00280 0.29 -0.8
56,683 - gspE OKFHMN_00280 0.29 +2.0
56,683 - gspE OKFHMN_00280 0.29 +0.2
56,683 - gspE OKFHMN_00280 0.29 -0.1
56,683 - gspE OKFHMN_00280 0.29 -0.7
56,686 + gspE OKFHMN_00280 0.30 -1.0
56,687 - gspE OKFHMN_00280 0.30 -0.5
56,687 - gspE OKFHMN_00280 0.30 -0.9
56,791 + gspE OKFHMN_00280 0.37 +0.9
56,871 - gspE OKFHMN_00280 0.42 +0.1
56,895 + gspE OKFHMN_00280 0.44 -2.1
56,895 + gspE OKFHMN_00280 0.44 +0.1
56,895 + gspE OKFHMN_00280 0.44 -1.3
56,896 - gspE OKFHMN_00280 0.44 +1.1
56,896 - gspE OKFHMN_00280 0.44 -0.6
56,926 + gspE OKFHMN_00280 0.46 +0.7
56,936 + gspE OKFHMN_00280 0.46 -0.1
56,936 + gspE OKFHMN_00280 0.46 -0.3
56,936 + gspE OKFHMN_00280 0.46 +0.8
57,069 + gspE OKFHMN_00280 0.55 -0.9
57,070 - gspE OKFHMN_00280 0.55 -0.2
57,079 + gspE OKFHMN_00280 0.56 +0.4
57,082 + gspE OKFHMN_00280 0.56 -0.3
57,083 - gspE OKFHMN_00280 0.56 -0.2
57,157 - gspE OKFHMN_00280 0.61 -1.0
57,222 + gspE OKFHMN_00280 0.65 -0.5
57,222 + gspE OKFHMN_00280 0.65 +1.6

Or see this region's nucleotide sequence