Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_16150 and OKGIIK_16155 are separated by 485 nucleotides OKGIIK_16155 and icdM are separated by 143 nucleotides
OKGIIK_16150: OKGIIK_16150 - Type III restriction enzyme, res subunit, at 3,537,671 to 3,540,514
_16150
OKGIIK_16155: OKGIIK_16155 - Helicase SNF2, at 3,541,000 to 3,544,356
_16155
OKGIIK_16160: icdM - NADP-dependent isocitrate dehydrogenase, at 3,544,500 to 3,546,725
icdM
Position (kb)
3540
3541
3542
3543
3544
3545 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3540.164 kb on + strand, within OKGIIK_16150 at 3540.164 kb on + strand, within OKGIIK_16150 at 3540.165 kb on - strand, within OKGIIK_16150 at 3540.165 kb on - strand, within OKGIIK_16150 at 3540.165 kb on - strand, within OKGIIK_16150 at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.267 kb on + strand at 3540.268 kb on - strand at 3540.268 kb on - strand at 3540.268 kb on - strand at 3540.268 kb on - strand at 3540.268 kb on - strand at 3540.268 kb on - strand at 3540.268 kb on - strand at 3540.356 kb on + strand at 3540.356 kb on + strand at 3540.414 kb on + strand at 3540.415 kb on - strand at 3540.415 kb on - strand at 3540.415 kb on - strand at 3540.415 kb on - strand at 3540.415 kb on - strand at 3540.552 kb on + strand at 3540.671 kb on + strand at 3540.671 kb on + strand at 3540.671 kb on + strand at 3540.672 kb on - strand at 3540.672 kb on - strand at 3540.672 kb on - strand at 3540.723 kb on + strand at 3540.723 kb on + strand at 3540.723 kb on + strand at 3540.723 kb on + strand at 3540.724 kb on - strand at 3540.724 kb on - strand at 3540.724 kb on - strand at 3540.724 kb on - strand at 3540.724 kb on - strand at 3540.731 kb on + strand at 3540.778 kb on + strand at 3540.778 kb on + strand at 3540.779 kb on - strand at 3540.784 kb on + strand at 3540.815 kb on + strand at 3540.815 kb on + strand at 3540.815 kb on + strand at 3540.816 kb on - strand at 3540.816 kb on - strand at 3540.891 kb on + strand at 3540.892 kb on - strand at 3540.892 kb on - strand at 3540.892 kb on - strand at 3541.001 kb on + strand at 3541.001 kb on + strand at 3541.002 kb on - strand at 3541.002 kb on - strand at 3541.002 kb on - strand at 3541.002 kb on - strand at 3541.002 kb on - strand at 3541.002 kb on - strand at 3541.110 kb on + strand at 3541.111 kb on - strand at 3541.154 kb on + strand at 3541.154 kb on + strand at 3541.154 kb on + strand at 3541.154 kb on + strand at 3541.155 kb on - strand at 3541.238 kb on + strand at 3541.238 kb on + strand at 3541.238 kb on + strand at 3541.682 kb on + strand, within OKGIIK_16155 at 3541.682 kb on + strand, within OKGIIK_16155 at 3541.683 kb on - strand, within OKGIIK_16155 at 3541.683 kb on - strand, within OKGIIK_16155 at 3541.850 kb on + strand, within OKGIIK_16155 at 3541.984 kb on + strand, within OKGIIK_16155 at 3542.096 kb on + strand, within OKGIIK_16155 at 3542.096 kb on + strand, within OKGIIK_16155 at 3542.097 kb on - strand, within OKGIIK_16155 at 3542.097 kb on - strand, within OKGIIK_16155 at 3542.097 kb on - strand, within OKGIIK_16155 at 3542.097 kb on - strand, within OKGIIK_16155 at 3542.170 kb on + strand, within OKGIIK_16155 at 3542.170 kb on + strand, within OKGIIK_16155 at 3542.170 kb on + strand, within OKGIIK_16155 at 3542.171 kb on - strand, within OKGIIK_16155 at 3542.171 kb on - strand, within OKGIIK_16155 at 3542.171 kb on - strand, within OKGIIK_16155 at 3542.729 kb on - strand, within OKGIIK_16155 at 3542.865 kb on - strand, within OKGIIK_16155 at 3542.996 kb on + strand, within OKGIIK_16155 at 3542.997 kb on - strand, within OKGIIK_16155 at 3542.997 kb on - strand, within OKGIIK_16155 at 3543.002 kb on + strand, within OKGIIK_16155 at 3543.002 kb on + strand, within OKGIIK_16155 at 3543.419 kb on + strand, within OKGIIK_16155 at 3543.419 kb on + strand, within OKGIIK_16155 at 3543.533 kb on + strand, within OKGIIK_16155 at 3543.534 kb on - strand, within OKGIIK_16155 at 3543.534 kb on - strand, within OKGIIK_16155 at 3543.614 kb on + strand, within OKGIIK_16155 at 3543.614 kb on + strand, within OKGIIK_16155 at 3543.614 kb on + strand, within OKGIIK_16155 at 3543.614 kb on + strand, within OKGIIK_16155 at 3543.614 kb on + strand, within OKGIIK_16155 at 3543.615 kb on - strand, within OKGIIK_16155 at 3543.615 kb on - strand, within OKGIIK_16155 at 3543.615 kb on - strand, within OKGIIK_16155 at 3543.680 kb on + strand, within OKGIIK_16155 at 3543.680 kb on + strand, within OKGIIK_16155 at 3543.681 kb on - strand, within OKGIIK_16155 at 3543.691 kb on + strand, within OKGIIK_16155 at 3543.691 kb on + strand, within OKGIIK_16155 at 3543.691 kb on + strand, within OKGIIK_16155 at 3543.691 kb on + strand, within OKGIIK_16155 at 3543.691 kb on + strand, within OKGIIK_16155 at 3543.692 kb on - strand, within OKGIIK_16155 at 3543.692 kb on - strand, within OKGIIK_16155 at 3543.692 kb on - strand, within OKGIIK_16155 at 3543.692 kb on - strand, within OKGIIK_16155 at 3543.692 kb on - strand, within OKGIIK_16155 at 3543.765 kb on + strand, within OKGIIK_16155 at 3544.175 kb on + strand at 3544.271 kb on + strand at 3544.524 kb on + strand at 3544.524 kb on + strand at 3544.524 kb on + strand at 3544.524 kb on + strand at 3544.524 kb on + strand at 3544.524 kb on + strand at 3544.524 kb on + strand at 3544.525 kb on - strand at 3544.525 kb on - strand at 3544.525 kb on - strand at 3544.816 kb on - strand, within icdM at 3544.962 kb on + strand, within icdM at 3544.962 kb on + strand, within icdM at 3544.962 kb on + strand, within icdM at 3544.962 kb on + strand, within icdM at 3544.962 kb on + strand, within icdM at 3544.963 kb on - strand, within icdM at 3544.963 kb on - strand, within icdM at 3544.963 kb on - strand, within icdM at 3545.046 kb on + strand, within icdM at 3545.046 kb on + strand, within icdM at 3545.046 kb on + strand, within icdM at 3545.047 kb on - strand, within icdM at 3545.047 kb on - strand, within icdM at 3545.128 kb on + strand, within icdM at 3545.128 kb on + strand, within icdM at 3545.128 kb on + strand, within icdM at 3545.128 kb on + strand, within icdM at 3545.129 kb on - strand, within icdM at 3545.129 kb on - strand, within icdM at 3545.129 kb on - strand, within icdM at 3545.325 kb on + strand, within icdM at 3545.325 kb on + strand, within icdM at 3545.326 kb on - strand, within icdM at 3545.326 kb on - strand, within icdM at 3545.326 kb on - strand, within icdM at 3545.326 kb on - strand, within icdM at 3545.326 kb on - strand, within icdM
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 3,540,164 + OKGIIK_16150 0.88 -1.6 3,540,164 + OKGIIK_16150 0.88 +1.9 3,540,165 - OKGIIK_16150 0.88 +1.1 3,540,165 - OKGIIK_16150 0.88 -0.6 3,540,165 - OKGIIK_16150 0.88 -1.7 3,540,267 + +0.6 3,540,267 + -0.5 3,540,267 + -0.3 3,540,267 + -0.4 3,540,267 + +1.2 3,540,267 + -2.0 3,540,267 + +0.2 3,540,267 + -1.7 3,540,267 + +0.3 3,540,267 + +1.9 3,540,267 + -0.9 3,540,268 - -1.4 3,540,268 - -0.3 3,540,268 - +0.2 3,540,268 - +1.4 3,540,268 - -0.5 3,540,268 - -2.1 3,540,268 - -0.2 3,540,356 + -0.5 3,540,356 + -0.7 3,540,414 + -0.7 3,540,415 - -1.2 3,540,415 - -2.2 3,540,415 - -0.9 3,540,415 - +1.0 3,540,415 - -1.4 3,540,552 + -0.3 3,540,671 + +1.1 3,540,671 + +0.3 3,540,671 + +0.4 3,540,672 - -1.3 3,540,672 - -0.3 3,540,672 - -0.2 3,540,723 + -0.4 3,540,723 + -1.0 3,540,723 + +0.5 3,540,723 + +1.1 3,540,724 - +1.4 3,540,724 - -3.4 3,540,724 - -1.5 3,540,724 - +1.4 3,540,724 - -1.9 3,540,731 + +0.7 3,540,778 + -1.6 3,540,778 + -0.6 3,540,779 - +0.8 3,540,784 + -0.7 3,540,815 + -1.9 3,540,815 + +2.1 3,540,815 + -0.5 3,540,816 - -0.9 3,540,816 - +1.7 3,540,891 + -1.2 3,540,892 - -1.2 3,540,892 - -2.5 3,540,892 - -0.2 3,541,001 + +0.6 3,541,001 + -1.2 3,541,002 - -0.6 3,541,002 - -0.5 3,541,002 - +1.2 3,541,002 - +2.0 3,541,002 - +0.8 3,541,002 - +0.0 3,541,110 + -0.5 3,541,111 - -0.5 3,541,154 + -1.7 3,541,154 + -1.4 3,541,154 + +0.6 3,541,154 + -0.5 3,541,155 - +0.4 3,541,238 + +0.2 3,541,238 + +0.2 3,541,238 + +0.0 3,541,682 + OKGIIK_16155 0.20 -0.9 3,541,682 + OKGIIK_16155 0.20 -0.7 3,541,683 - OKGIIK_16155 0.20 -1.6 3,541,683 - OKGIIK_16155 0.20 -1.2 3,541,850 + OKGIIK_16155 0.25 -0.2 3,541,984 + OKGIIK_16155 0.29 -0.6 3,542,096 + OKGIIK_16155 0.33 +0.9 3,542,096 + OKGIIK_16155 0.33 -0.2 3,542,097 - OKGIIK_16155 0.33 +0.2 3,542,097 - OKGIIK_16155 0.33 -0.8 3,542,097 - OKGIIK_16155 0.33 -0.9 3,542,097 - OKGIIK_16155 0.33 -0.4 3,542,170 + OKGIIK_16155 0.35 -0.5 3,542,170 + OKGIIK_16155 0.35 -0.1 3,542,170 + OKGIIK_16155 0.35 +1.0 3,542,171 - OKGIIK_16155 0.35 +0.9 3,542,171 - OKGIIK_16155 0.35 -0.9 3,542,171 - OKGIIK_16155 0.35 +0.1 3,542,729 - OKGIIK_16155 0.52 +0.4 3,542,865 - OKGIIK_16155 0.56 -0.2 3,542,996 + OKGIIK_16155 0.59 +0.0 3,542,997 - OKGIIK_16155 0.59 +0.6 3,542,997 - OKGIIK_16155 0.59 +0.4 3,543,002 + OKGIIK_16155 0.60 -0.5 3,543,002 + OKGIIK_16155 0.60 -1.0 3,543,419 + OKGIIK_16155 0.72 +1.0 3,543,419 + OKGIIK_16155 0.72 +1.2 3,543,533 + OKGIIK_16155 0.75 -0.5 3,543,534 - OKGIIK_16155 0.75 +0.8 3,543,534 - OKGIIK_16155 0.75 -0.8 3,543,614 + OKGIIK_16155 0.78 -0.9 3,543,614 + OKGIIK_16155 0.78 +0.4 3,543,614 + OKGIIK_16155 0.78 -0.1 3,543,614 + OKGIIK_16155 0.78 +1.0 3,543,614 + OKGIIK_16155 0.78 -0.5 3,543,615 - OKGIIK_16155 0.78 +0.6 3,543,615 - OKGIIK_16155 0.78 -1.2 3,543,615 - OKGIIK_16155 0.78 +0.1 3,543,680 + OKGIIK_16155 0.80 +0.2 3,543,680 + OKGIIK_16155 0.80 -0.7 3,543,681 - OKGIIK_16155 0.80 +0.1 3,543,691 + OKGIIK_16155 0.80 +0.8 3,543,691 + OKGIIK_16155 0.80 +0.3 3,543,691 + OKGIIK_16155 0.80 -0.4 3,543,691 + OKGIIK_16155 0.80 -0.4 3,543,691 + OKGIIK_16155 0.80 -2.5 3,543,692 - OKGIIK_16155 0.80 -1.6 3,543,692 - OKGIIK_16155 0.80 -0.6 3,543,692 - OKGIIK_16155 0.80 +1.1 3,543,692 - OKGIIK_16155 0.80 +1.3 3,543,692 - OKGIIK_16155 0.80 +1.8 3,543,765 + OKGIIK_16155 0.82 -0.2 3,544,175 + +0.0 3,544,271 + -1.1 3,544,524 + -1.7 3,544,524 + +0.4 3,544,524 + -1.4 3,544,524 + +0.6 3,544,524 + +0.2 3,544,524 + -0.6 3,544,524 + -2.0 3,544,525 - -0.4 3,544,525 - -0.6 3,544,525 - -1.2 3,544,816 - icdM OKGIIK_16160 0.14 -0.2 3,544,962 + icdM OKGIIK_16160 0.21 -0.7 3,544,962 + icdM OKGIIK_16160 0.21 -2.1 3,544,962 + icdM OKGIIK_16160 0.21 +0.4 3,544,962 + icdM OKGIIK_16160 0.21 -0.9 3,544,962 + icdM OKGIIK_16160 0.21 +0.1 3,544,963 - icdM OKGIIK_16160 0.21 -0.6 3,544,963 - icdM OKGIIK_16160 0.21 -0.7 3,544,963 - icdM OKGIIK_16160 0.21 +1.5 3,545,046 + icdM OKGIIK_16160 0.25 +1.5 3,545,046 + icdM OKGIIK_16160 0.25 +0.4 3,545,046 + icdM OKGIIK_16160 0.25 +0.0 3,545,047 - icdM OKGIIK_16160 0.25 -1.0 3,545,047 - icdM OKGIIK_16160 0.25 +0.2 3,545,128 + icdM OKGIIK_16160 0.28 +0.7 3,545,128 + icdM OKGIIK_16160 0.28 -2.4 3,545,128 + icdM OKGIIK_16160 0.28 -2.2 3,545,128 + icdM OKGIIK_16160 0.28 -1.2 3,545,129 - icdM OKGIIK_16160 0.28 -1.7 3,545,129 - icdM OKGIIK_16160 0.28 +0.9 3,545,129 - icdM OKGIIK_16160 0.28 +0.4 3,545,325 + icdM OKGIIK_16160 0.37 +0.5 3,545,325 + icdM OKGIIK_16160 0.37 +0.7 3,545,326 - icdM OKGIIK_16160 0.37 -0.2 3,545,326 - icdM OKGIIK_16160 0.37 +0.7 3,545,326 - icdM OKGIIK_16160 0.37 -1.9 3,545,326 - icdM OKGIIK_16160 0.37 -2.3 3,545,326 - icdM OKGIIK_16160 0.37 -1.3
Or see this region's nucleotide sequence