Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysQ and nudE overlap by 4 nucleotides nudE and suhB are separated by 23 nucleotides suhB and OKGIIK_11010 are separated by 15 nucleotides OKGIIK_11010 and bioA are separated by 70 nucleotides
OKGIIK_10995: cysQ - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 2,354,293 to 2,355,114
cysQ
OKGIIK_11000: nudE - ADP compounds hydrolase NudE, at 2,355,111 to 2,355,668
nudE
OKGIIK_11005: suhB - Archaeal fructose-1,6-bisphosphatase or related enzyme, inositol monophosphatase family, at 2,355,692 to 2,356,468
suhB
OKGIIK_11010: OKGIIK_11010 - Type II secretion system protein N, at 2,356,484 to 2,357,269
_11010
OKGIIK_11015: bioA - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 2,357,340 to 2,358,731
bioA
Position (kb)
2355
2356
2357 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.729 kb on + strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.730 kb on - strand, within cysQ at 2354.864 kb on + strand, within cysQ at 2354.864 kb on + strand, within cysQ at 2354.864 kb on + strand, within cysQ at 2354.865 kb on - strand, within cysQ at 2354.865 kb on - strand, within cysQ at 2355.038 kb on + strand at 2355.038 kb on + strand at 2355.038 kb on + strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.039 kb on - strand at 2355.139 kb on + strand at 2355.139 kb on + strand at 2355.139 kb on + strand at 2355.140 kb on - strand at 2355.140 kb on - strand at 2355.140 kb on - strand at 2355.140 kb on - strand at 2355.140 kb on - strand at 2355.277 kb on + strand, within nudE at 2355.277 kb on + strand, within nudE at 2355.277 kb on + strand, within nudE at 2355.277 kb on + strand, within nudE at 2355.278 kb on - strand, within nudE at 2355.278 kb on - strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.319 kb on + strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.320 kb on - strand, within nudE at 2355.457 kb on + strand, within nudE at 2355.457 kb on + strand, within nudE at 2355.478 kb on + strand, within nudE at 2355.479 kb on - strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.562 kb on + strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.563 kb on - strand, within nudE at 2355.657 kb on + strand at 2355.657 kb on + strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.658 kb on - strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.679 kb on + strand at 2355.680 kb on - strand at 2355.680 kb on - strand at 2355.680 kb on - strand at 2355.680 kb on - strand at 2355.680 kb on - strand at 2355.897 kb on + strand, within suhB at 2355.897 kb on + strand, within suhB at 2355.897 kb on + strand, within suhB at 2355.897 kb on + strand, within suhB at 2355.897 kb on + strand, within suhB at 2355.898 kb on - strand, within suhB at 2356.081 kb on + strand, within suhB at 2356.101 kb on + strand, within suhB at 2356.101 kb on + strand, within suhB at 2356.102 kb on - strand, within suhB at 2356.102 kb on - strand, within suhB at 2356.266 kb on + strand, within suhB at 2356.267 kb on - strand, within suhB at 2356.390 kb on - strand, within suhB at 2356.390 kb on - strand, within suhB at 2356.390 kb on - strand, within suhB at 2356.390 kb on - strand, within suhB at 2356.390 kb on - strand, within suhB at 2356.775 kb on - strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.899 kb on + strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2356.900 kb on - strand, within OKGIIK_11010 at 2357.333 kb on + strand at 2357.333 kb on + strand at 2357.334 kb on - strand at 2357.334 kb on - strand at 2357.334 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 2,354,729 + cysQ OKGIIK_10995 0.53 -0.5 2,354,729 + cysQ OKGIIK_10995 0.53 +1.3 2,354,729 + cysQ OKGIIK_10995 0.53 +1.1 2,354,729 + cysQ OKGIIK_10995 0.53 -0.1 2,354,729 + cysQ OKGIIK_10995 0.53 -0.6 2,354,729 + cysQ OKGIIK_10995 0.53 -1.9 2,354,729 + cysQ OKGIIK_10995 0.53 +1.0 2,354,729 + cysQ OKGIIK_10995 0.53 -1.9 2,354,729 + cysQ OKGIIK_10995 0.53 -1.5 2,354,729 + cysQ OKGIIK_10995 0.53 +0.5 2,354,729 + cysQ OKGIIK_10995 0.53 +0.9 2,354,729 + cysQ OKGIIK_10995 0.53 -1.1 2,354,729 + cysQ OKGIIK_10995 0.53 -1.9 2,354,729 + cysQ OKGIIK_10995 0.53 -1.9 2,354,729 + cysQ OKGIIK_10995 0.53 +0.3 2,354,729 + cysQ OKGIIK_10995 0.53 +0.9 2,354,729 + cysQ OKGIIK_10995 0.53 +0.3 2,354,729 + cysQ OKGIIK_10995 0.53 -0.7 2,354,730 - cysQ OKGIIK_10995 0.53 +2.1 2,354,730 - cysQ OKGIIK_10995 0.53 +0.4 2,354,730 - cysQ OKGIIK_10995 0.53 -1.1 2,354,730 - cysQ OKGIIK_10995 0.53 -1.9 2,354,730 - cysQ OKGIIK_10995 0.53 -0.1 2,354,730 - cysQ OKGIIK_10995 0.53 +0.5 2,354,730 - cysQ OKGIIK_10995 0.53 -1.0 2,354,730 - cysQ OKGIIK_10995 0.53 -1.1 2,354,730 - cysQ OKGIIK_10995 0.53 -1.5 2,354,730 - cysQ OKGIIK_10995 0.53 -0.1 2,354,730 - cysQ OKGIIK_10995 0.53 -1.1 2,354,730 - cysQ OKGIIK_10995 0.53 +0.8 2,354,730 - cysQ OKGIIK_10995 0.53 -1.1 2,354,730 - cysQ OKGIIK_10995 0.53 -0.1 2,354,730 - cysQ OKGIIK_10995 0.53 -1.1 2,354,730 - cysQ OKGIIK_10995 0.53 -0.3 2,354,730 - cysQ OKGIIK_10995 0.53 -3.6 2,354,730 - cysQ OKGIIK_10995 0.53 -0.5 2,354,730 - cysQ OKGIIK_10995 0.53 -0.7 2,354,864 + cysQ OKGIIK_10995 0.69 +0.3 2,354,864 + cysQ OKGIIK_10995 0.69 -0.4 2,354,864 + cysQ OKGIIK_10995 0.69 -0.2 2,354,865 - cysQ OKGIIK_10995 0.70 +0.8 2,354,865 - cysQ OKGIIK_10995 0.70 +0.9 2,355,038 + -0.2 2,355,038 + -1.5 2,355,038 + -1.8 2,355,039 - -1.2 2,355,039 - +0.7 2,355,039 - +1.3 2,355,039 - +0.6 2,355,039 - -1.1 2,355,039 - +0.0 2,355,039 - -1.2 2,355,139 + +1.0 2,355,139 + -1.1 2,355,139 + -2.1 2,355,140 - -0.1 2,355,140 - +0.5 2,355,140 - -2.3 2,355,140 - -0.5 2,355,140 - +0.4 2,355,277 + nudE OKGIIK_11000 0.30 -0.1 2,355,277 + nudE OKGIIK_11000 0.30 -0.5 2,355,277 + nudE OKGIIK_11000 0.30 -0.7 2,355,277 + nudE OKGIIK_11000 0.30 -0.7 2,355,278 - nudE OKGIIK_11000 0.30 -0.6 2,355,278 - nudE OKGIIK_11000 0.30 -0.3 2,355,319 + nudE OKGIIK_11000 0.37 -1.4 2,355,319 + nudE OKGIIK_11000 0.37 +0.5 2,355,319 + nudE OKGIIK_11000 0.37 +1.1 2,355,319 + nudE OKGIIK_11000 0.37 -0.7 2,355,319 + nudE OKGIIK_11000 0.37 -1.3 2,355,319 + nudE OKGIIK_11000 0.37 -1.3 2,355,319 + nudE OKGIIK_11000 0.37 -1.3 2,355,319 + nudE OKGIIK_11000 0.37 -0.5 2,355,319 + nudE OKGIIK_11000 0.37 +0.9 2,355,319 + nudE OKGIIK_11000 0.37 -0.3 2,355,319 + nudE OKGIIK_11000 0.37 -1.3 2,355,319 + nudE OKGIIK_11000 0.37 -2.2 2,355,319 + nudE OKGIIK_11000 0.37 +1.6 2,355,319 + nudE OKGIIK_11000 0.37 -0.5 2,355,319 + nudE OKGIIK_11000 0.37 -0.5 2,355,319 + nudE OKGIIK_11000 0.37 -2.1 2,355,319 + nudE OKGIIK_11000 0.37 -0.5 2,355,319 + nudE OKGIIK_11000 0.37 -1.5 2,355,319 + nudE OKGIIK_11000 0.37 +0.5 2,355,319 + nudE OKGIIK_11000 0.37 -1.2 2,355,319 + nudE OKGIIK_11000 0.37 +0.4 2,355,319 + nudE OKGIIK_11000 0.37 -1.9 2,355,319 + nudE OKGIIK_11000 0.37 -0.7 2,355,319 + nudE OKGIIK_11000 0.37 +1.3 2,355,319 + nudE OKGIIK_11000 0.37 -2.1 2,355,319 + nudE OKGIIK_11000 0.37 -0.5 2,355,319 + nudE OKGIIK_11000 0.37 +1.5 2,355,319 + nudE OKGIIK_11000 0.37 +0.1 2,355,319 + nudE OKGIIK_11000 0.37 -1.4 2,355,319 + nudE OKGIIK_11000 0.37 -0.7 2,355,319 + nudE OKGIIK_11000 0.37 -2.8 2,355,319 + nudE OKGIIK_11000 0.37 -0.1 2,355,320 - nudE OKGIIK_11000 0.37 -0.7 2,355,320 - nudE OKGIIK_11000 0.37 +0.1 2,355,320 - nudE OKGIIK_11000 0.37 +1.1 2,355,320 - nudE OKGIIK_11000 0.37 -1.9 2,355,320 - nudE OKGIIK_11000 0.37 -0.0 2,355,320 - nudE OKGIIK_11000 0.37 +0.1 2,355,320 - nudE OKGIIK_11000 0.37 -0.7 2,355,320 - nudE OKGIIK_11000 0.37 -0.1 2,355,320 - nudE OKGIIK_11000 0.37 -1.9 2,355,320 - nudE OKGIIK_11000 0.37 -1.9 2,355,320 - nudE OKGIIK_11000 0.37 +0.3 2,355,320 - nudE OKGIIK_11000 0.37 +0.4 2,355,320 - nudE OKGIIK_11000 0.37 -1.5 2,355,320 - nudE OKGIIK_11000 0.37 +0.9 2,355,320 - nudE OKGIIK_11000 0.37 +0.1 2,355,320 - nudE OKGIIK_11000 0.37 +0.7 2,355,320 - nudE OKGIIK_11000 0.37 -0.7 2,355,320 - nudE OKGIIK_11000 0.37 -0.1 2,355,320 - nudE OKGIIK_11000 0.37 -0.7 2,355,320 - nudE OKGIIK_11000 0.37 -0.7 2,355,320 - nudE OKGIIK_11000 0.37 -1.1 2,355,320 - nudE OKGIIK_11000 0.37 +0.3 2,355,320 - nudE OKGIIK_11000 0.37 -0.7 2,355,320 - nudE OKGIIK_11000 0.37 +0.0 2,355,320 - nudE OKGIIK_11000 0.37 +1.0 2,355,320 - nudE OKGIIK_11000 0.37 +0.3 2,355,320 - nudE OKGIIK_11000 0.37 -1.5 2,355,320 - nudE OKGIIK_11000 0.37 +0.7 2,355,320 - nudE OKGIIK_11000 0.37 -0.1 2,355,320 - nudE OKGIIK_11000 0.37 -0.2 2,355,457 + nudE OKGIIK_11000 0.62 -0.5 2,355,457 + nudE OKGIIK_11000 0.62 +0.2 2,355,478 + nudE OKGIIK_11000 0.66 +0.4 2,355,479 - nudE OKGIIK_11000 0.66 +0.3 2,355,562 + nudE OKGIIK_11000 0.81 -0.3 2,355,562 + nudE OKGIIK_11000 0.81 -1.1 2,355,562 + nudE OKGIIK_11000 0.81 +0.3 2,355,562 + nudE OKGIIK_11000 0.81 -0.3 2,355,562 + nudE OKGIIK_11000 0.81 -2.7 2,355,562 + nudE OKGIIK_11000 0.81 +0.7 2,355,562 + nudE OKGIIK_11000 0.81 +0.8 2,355,562 + nudE OKGIIK_11000 0.81 +0.1 2,355,562 + nudE OKGIIK_11000 0.81 +1.5 2,355,562 + nudE OKGIIK_11000 0.81 -1.1 2,355,563 - nudE OKGIIK_11000 0.81 -0.3 2,355,563 - nudE OKGIIK_11000 0.81 -0.7 2,355,563 - nudE OKGIIK_11000 0.81 -0.7 2,355,563 - nudE OKGIIK_11000 0.81 -0.3 2,355,563 - nudE OKGIIK_11000 0.81 -2.7 2,355,563 - nudE OKGIIK_11000 0.81 +0.4 2,355,563 - nudE OKGIIK_11000 0.81 -2.2 2,355,563 - nudE OKGIIK_11000 0.81 -1.5 2,355,563 - nudE OKGIIK_11000 0.81 -1.3 2,355,563 - nudE OKGIIK_11000 0.81 -1.4 2,355,563 - nudE OKGIIK_11000 0.81 -1.1 2,355,563 - nudE OKGIIK_11000 0.81 -0.1 2,355,563 - nudE OKGIIK_11000 0.81 +0.0 2,355,563 - nudE OKGIIK_11000 0.81 +0.7 2,355,563 - nudE OKGIIK_11000 0.81 -0.1 2,355,563 - nudE OKGIIK_11000 0.81 -2.3 2,355,657 + -1.5 2,355,657 + -1.1 2,355,658 - -0.9 2,355,658 - -0.1 2,355,658 - -0.4 2,355,658 - -0.1 2,355,658 - -0.3 2,355,658 - -0.5 2,355,658 - -1.9 2,355,658 - -1.3 2,355,658 - +0.6 2,355,679 + +0.3 2,355,679 + -1.5 2,355,679 + -1.2 2,355,679 + +0.3 2,355,679 + +0.3 2,355,679 + -1.1 2,355,679 + +1.6 2,355,679 + -0.2 2,355,679 + -0.5 2,355,680 - -2.4 2,355,680 - +0.9 2,355,680 - +1.6 2,355,680 - +0.6 2,355,680 - -1.1 2,355,897 + suhB OKGIIK_11005 0.26 -2.3 2,355,897 + suhB OKGIIK_11005 0.26 -1.3 2,355,897 + suhB OKGIIK_11005 0.26 -1.5 2,355,897 + suhB OKGIIK_11005 0.26 -1.7 2,355,897 + suhB OKGIIK_11005 0.26 -0.7 2,355,898 - suhB OKGIIK_11005 0.27 -1.3 2,356,081 + suhB OKGIIK_11005 0.50 -1.9 2,356,101 + suhB OKGIIK_11005 0.53 -1.5 2,356,101 + suhB OKGIIK_11005 0.53 -0.2 2,356,102 - suhB OKGIIK_11005 0.53 -1.1 2,356,102 - suhB OKGIIK_11005 0.53 -0.7 2,356,266 + suhB OKGIIK_11005 0.74 -1.4 2,356,267 - suhB OKGIIK_11005 0.74 -1.0 2,356,390 - suhB OKGIIK_11005 0.90 -0.7 2,356,390 - suhB OKGIIK_11005 0.90 -0.7 2,356,390 - suhB OKGIIK_11005 0.90 -2.4 2,356,390 - suhB OKGIIK_11005 0.90 -2.2 2,356,390 - suhB OKGIIK_11005 0.90 -0.7 2,356,775 - OKGIIK_11010 0.37 +0.3 2,356,899 + OKGIIK_11010 0.53 -0.3 2,356,899 + OKGIIK_11010 0.53 -0.7 2,356,899 + OKGIIK_11010 0.53 -0.3 2,356,899 + OKGIIK_11010 0.53 -2.1 2,356,899 + OKGIIK_11010 0.53 -0.6 2,356,899 + OKGIIK_11010 0.53 -0.8 2,356,900 - OKGIIK_11010 0.53 -0.7 2,356,900 - OKGIIK_11010 0.53 -0.3 2,356,900 - OKGIIK_11010 0.53 -0.3 2,356,900 - OKGIIK_11010 0.53 -1.3 2,356,900 - OKGIIK_11010 0.53 -0.9 2,356,900 - OKGIIK_11010 0.53 +1.3 2,356,900 - OKGIIK_11010 0.53 +0.4 2,357,333 + -0.7 2,357,333 + -1.7 2,357,334 - -2.1 2,357,334 - -1.6 2,357,334 - +0.6
Or see this region's nucleotide sequence