Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaF and OKGIIK_05625 are separated by 35 nucleotides OKGIIK_05625 and elaC overlap by 4 nucleotides elaC and Ser_trna are separated by 92 nucleotides
OKGIIK_05620: rfaF - ADP-heptose--LPS heptosyltransferase, at 1,239,294 to 1,240,352
rfaF
OKGIIK_05625: OKGIIK_05625 - 3-deoxy-D-manno-octulosonic acid kinase, at 1,240,388 to 1,241,119
_05625
OKGIIK_05630: elaC - Ribonuclease BN, tRNA processing enzyme, at 1,241,116 to 1,241,874
elaC
OKGIIK_05635: Ser_trna - tRNA-Ser, at 1,241,967 to 1,242,056
_trna
Position (kb)
1240
1241
1242 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.475 kb on + strand, within rfaF at 1239.475 kb on + strand, within rfaF at 1239.476 kb on - strand, within rfaF at 1239.476 kb on - strand, within rfaF at 1239.757 kb on + strand, within rfaF at 1239.757 kb on + strand, within rfaF at 1239.758 kb on - strand, within rfaF at 1240.365 kb on - strand at 1240.365 kb on - strand at 1240.365 kb on - strand at 1240.365 kb on - strand at 1240.487 kb on + strand, within OKGIIK_05625 at 1240.487 kb on + strand, within OKGIIK_05625 at 1240.488 kb on - strand, within OKGIIK_05625 at 1240.488 kb on - strand, within OKGIIK_05625 at 1240.583 kb on + strand, within OKGIIK_05625 at 1240.583 kb on + strand, within OKGIIK_05625 at 1240.583 kb on + strand, within OKGIIK_05625 at 1240.584 kb on - strand, within OKGIIK_05625 at 1240.584 kb on - strand, within OKGIIK_05625 at 1240.625 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.730 kb on + strand, within OKGIIK_05625 at 1240.731 kb on - strand, within OKGIIK_05625 at 1240.751 kb on + strand, within OKGIIK_05625 at 1240.752 kb on - strand, within OKGIIK_05625 at 1240.752 kb on - strand, within OKGIIK_05625 at 1240.752 kb on - strand, within OKGIIK_05625 at 1240.752 kb on - strand, within OKGIIK_05625 at 1240.861 kb on - strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.937 kb on + strand, within OKGIIK_05625 at 1240.938 kb on - strand, within OKGIIK_05625 at 1240.938 kb on - strand, within OKGIIK_05625 at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.090 kb on + strand at 1241.091 kb on - strand at 1241.091 kb on - strand at 1241.091 kb on - strand at 1241.097 kb on - strand at 1241.104 kb on + strand at 1241.104 kb on + strand at 1241.104 kb on + strand at 1241.248 kb on + strand, within elaC at 1241.249 kb on - strand, within elaC at 1241.249 kb on - strand, within elaC at 1241.261 kb on - strand, within elaC at 1241.564 kb on - strand, within elaC at 1241.594 kb on - strand, within elaC at 1241.709 kb on + strand, within elaC at 1241.722 kb on + strand, within elaC at 1241.755 kb on + strand, within elaC at 1241.756 kb on - strand, within elaC at 1241.756 kb on - strand, within elaC at 1241.761 kb on + strand, within elaC at 1241.762 kb on - strand, within elaC
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 1,239,445 + rfaF OKGIIK_05620 0.14 +0.7 1,239,445 + rfaF OKGIIK_05620 0.14 -0.9 1,239,445 + rfaF OKGIIK_05620 0.14 -1.8 1,239,445 + rfaF OKGIIK_05620 0.14 -1.1 1,239,445 + rfaF OKGIIK_05620 0.14 -1.8 1,239,445 + rfaF OKGIIK_05620 0.14 -0.9 1,239,446 - rfaF OKGIIK_05620 0.14 -0.3 1,239,446 - rfaF OKGIIK_05620 0.14 -1.3 1,239,446 - rfaF OKGIIK_05620 0.14 +0.2 1,239,446 - rfaF OKGIIK_05620 0.14 -2.0 1,239,446 - rfaF OKGIIK_05620 0.14 -1.3 1,239,446 - rfaF OKGIIK_05620 0.14 -0.7 1,239,446 - rfaF OKGIIK_05620 0.14 -1.3 1,239,475 + rfaF OKGIIK_05620 0.17 -1.0 1,239,475 + rfaF OKGIIK_05620 0.17 -1.1 1,239,476 - rfaF OKGIIK_05620 0.17 -0.7 1,239,476 - rfaF OKGIIK_05620 0.17 -1.8 1,239,757 + rfaF OKGIIK_05620 0.44 -1.4 1,239,757 + rfaF OKGIIK_05620 0.44 -1.4 1,239,758 - rfaF OKGIIK_05620 0.44 -1.7 1,240,365 - -1.1 1,240,365 - +1.0 1,240,365 - +2.0 1,240,365 - +0.7 1,240,487 + OKGIIK_05625 0.14 +1.0 1,240,487 + OKGIIK_05625 0.14 -0.4 1,240,488 - OKGIIK_05625 0.14 +1.1 1,240,488 - OKGIIK_05625 0.14 +1.1 1,240,583 + OKGIIK_05625 0.27 +1.4 1,240,583 + OKGIIK_05625 0.27 +0.4 1,240,583 + OKGIIK_05625 0.27 -2.0 1,240,584 - OKGIIK_05625 0.27 -0.3 1,240,584 - OKGIIK_05625 0.27 +0.3 1,240,625 + OKGIIK_05625 0.32 -1.1 1,240,730 + OKGIIK_05625 0.47 +0.1 1,240,730 + OKGIIK_05625 0.47 -0.5 1,240,730 + OKGIIK_05625 0.47 +0.1 1,240,730 + OKGIIK_05625 0.47 -1.0 1,240,730 + OKGIIK_05625 0.47 +0.1 1,240,731 - OKGIIK_05625 0.47 -0.3 1,240,751 + OKGIIK_05625 0.50 -0.9 1,240,752 - OKGIIK_05625 0.50 +0.0 1,240,752 - OKGIIK_05625 0.50 +0.3 1,240,752 - OKGIIK_05625 0.50 -1.7 1,240,752 - OKGIIK_05625 0.50 -1.7 1,240,861 - OKGIIK_05625 0.65 -0.1 1,240,937 + OKGIIK_05625 0.75 +0.8 1,240,937 + OKGIIK_05625 0.75 +1.4 1,240,937 + OKGIIK_05625 0.75 -0.7 1,240,937 + OKGIIK_05625 0.75 +0.4 1,240,937 + OKGIIK_05625 0.75 +0.2 1,240,937 + OKGIIK_05625 0.75 +0.3 1,240,937 + OKGIIK_05625 0.75 +0.7 1,240,937 + OKGIIK_05625 0.75 +1.8 1,240,937 + OKGIIK_05625 0.75 +0.4 1,240,938 - OKGIIK_05625 0.75 +0.7 1,240,938 - OKGIIK_05625 0.75 +0.3 1,241,090 + +1.4 1,241,090 + -0.9 1,241,090 + +1.7 1,241,090 + -0.3 1,241,090 + +0.8 1,241,090 + +0.2 1,241,090 + +1.3 1,241,090 + +0.1 1,241,091 - -0.5 1,241,091 - -0.7 1,241,091 - +0.7 1,241,097 - +0.6 1,241,104 + +0.4 1,241,104 + -0.7 1,241,104 + +0.3 1,241,248 + elaC OKGIIK_05630 0.17 +0.1 1,241,249 - elaC OKGIIK_05630 0.18 -0.6 1,241,249 - elaC OKGIIK_05630 0.18 -0.3 1,241,261 - elaC OKGIIK_05630 0.19 -0.8 1,241,564 - elaC OKGIIK_05630 0.59 -1.9 1,241,594 - elaC OKGIIK_05630 0.63 -1.0 1,241,709 + elaC OKGIIK_05630 0.78 -0.9 1,241,722 + elaC OKGIIK_05630 0.80 -0.3 1,241,755 + elaC OKGIIK_05630 0.84 -1.9 1,241,756 - elaC OKGIIK_05630 0.84 +0.4 1,241,756 - elaC OKGIIK_05630 0.84 -1.1 1,241,761 + elaC OKGIIK_05630 0.85 -0.9 1,241,762 - elaC OKGIIK_05630 0.85 -0.3
Or see this region's nucleotide sequence