Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisS and hutH are separated by 62 nucleotides hutH and OKGIIK_05615 are separated by 28 nucleotides OKGIIK_05615 and rfaF are separated by 82 nucleotides
OKGIIK_05605: hisS - histidine--tRNA ligase, at 1,235,441 to 1,236,820
hisS
OKGIIK_05610: hutH - histidine ammonia-lyase, at 1,236,883 to 1,238,784
hutH
OKGIIK_05615: OKGIIK_05615 - DUF6165 domain-containing protein, at 1,238,813 to 1,239,211
_05615
OKGIIK_05620: rfaF - ADP-heptose--LPS heptosyltransferase, at 1,239,294 to 1,240,352
rfaF
Position (kb)
1236
1237
1238
1239 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1236.897 kb on + strand at 1236.897 kb on + strand at 1237.051 kb on + strand at 1237.051 kb on + strand at 1237.051 kb on + strand at 1237.052 kb on - strand at 1237.052 kb on - strand at 1237.199 kb on + strand, within hutH at 1237.199 kb on + strand, within hutH at 1237.199 kb on + strand, within hutH at 1237.199 kb on + strand, within hutH at 1237.199 kb on + strand, within hutH at 1237.200 kb on - strand, within hutH at 1237.200 kb on - strand, within hutH at 1237.435 kb on + strand, within hutH at 1237.436 kb on - strand, within hutH at 1237.436 kb on - strand, within hutH at 1237.436 kb on - strand, within hutH at 1237.753 kb on + strand, within hutH at 1237.753 kb on + strand, within hutH at 1237.754 kb on - strand, within hutH at 1237.754 kb on - strand, within hutH at 1237.882 kb on + strand, within hutH at 1237.882 kb on + strand, within hutH at 1237.882 kb on + strand, within hutH at 1237.882 kb on + strand, within hutH at 1237.882 kb on + strand, within hutH at 1237.882 kb on + strand, within hutH at 1237.883 kb on - strand, within hutH at 1237.883 kb on - strand, within hutH at 1237.883 kb on - strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.936 kb on + strand, within hutH at 1237.937 kb on - strand, within hutH at 1237.937 kb on - strand, within hutH at 1237.937 kb on - strand, within hutH at 1237.937 kb on - strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.137 kb on + strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.138 kb on - strand, within hutH at 1238.281 kb on + strand, within hutH at 1238.281 kb on + strand, within hutH at 1238.281 kb on + strand, within hutH at 1238.281 kb on + strand, within hutH at 1238.281 kb on + strand, within hutH at 1238.281 kb on + strand, within hutH at 1238.281 kb on + strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.282 kb on - strand, within hutH at 1238.338 kb on + strand, within hutH at 1238.338 kb on + strand, within hutH at 1238.338 kb on + strand, within hutH at 1238.338 kb on + strand, within hutH at 1238.338 kb on + strand, within hutH at 1238.338 kb on + strand, within hutH at 1238.338 kb on + strand, within hutH at 1238.339 kb on - strand, within hutH at 1238.339 kb on - strand, within hutH at 1238.339 kb on - strand, within hutH at 1238.339 kb on - strand, within hutH at 1238.339 kb on - strand, within hutH at 1238.339 kb on - strand, within hutH at 1238.339 kb on - strand, within hutH at 1238.452 kb on + strand, within hutH at 1238.452 kb on + strand, within hutH at 1238.452 kb on + strand, within hutH at 1238.452 kb on + strand, within hutH at 1238.452 kb on + strand, within hutH at 1238.452 kb on + strand, within hutH at 1238.452 kb on + strand, within hutH at 1238.453 kb on - strand, within hutH at 1238.453 kb on - strand, within hutH at 1238.453 kb on - strand, within hutH at 1238.453 kb on - strand, within hutH at 1238.453 kb on - strand, within hutH at 1238.453 kb on - strand, within hutH at 1238.582 kb on - strand, within hutH at 1239.107 kb on + strand, within OKGIIK_05615 at 1239.107 kb on + strand, within OKGIIK_05615 at 1239.107 kb on + strand, within OKGIIK_05615 at 1239.107 kb on + strand, within OKGIIK_05615 at 1239.107 kb on + strand, within OKGIIK_05615 at 1239.107 kb on + strand, within OKGIIK_05615 at 1239.107 kb on + strand, within OKGIIK_05615 at 1239.108 kb on - strand, within OKGIIK_05615 at 1239.108 kb on - strand, within OKGIIK_05615 at 1239.108 kb on - strand, within OKGIIK_05615 at 1239.108 kb on - strand, within OKGIIK_05615 at 1239.108 kb on - strand, within OKGIIK_05615 at 1239.108 kb on - strand, within OKGIIK_05615 at 1239.198 kb on - strand at 1239.223 kb on - strand at 1239.295 kb on + strand at 1239.295 kb on + strand at 1239.295 kb on + strand at 1239.295 kb on + strand at 1239.295 kb on + strand at 1239.295 kb on + strand at 1239.295 kb on + strand at 1239.295 kb on + strand at 1239.328 kb on + strand at 1239.328 kb on + strand at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.445 kb on + strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.446 kb on - strand, within rfaF at 1239.475 kb on + strand, within rfaF at 1239.475 kb on + strand, within rfaF at 1239.476 kb on - strand, within rfaF at 1239.476 kb on - strand, within rfaF at 1239.757 kb on + strand, within rfaF at 1239.757 kb on + strand, within rfaF at 1239.758 kb on - strand, within rfaF
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 1,236,897 + +0.7 1,236,897 + +0.9 1,237,051 + -0.2 1,237,051 + -0.3 1,237,051 + +1.3 1,237,052 - +1.1 1,237,052 - -0.7 1,237,199 + hutH OKGIIK_05610 0.17 +0.7 1,237,199 + hutH OKGIIK_05610 0.17 +0.9 1,237,199 + hutH OKGIIK_05610 0.17 -0.7 1,237,199 + hutH OKGIIK_05610 0.17 -1.5 1,237,199 + hutH OKGIIK_05610 0.17 -2.2 1,237,200 - hutH OKGIIK_05610 0.17 +1.2 1,237,200 - hutH OKGIIK_05610 0.17 -0.4 1,237,435 + hutH OKGIIK_05610 0.29 +1.1 1,237,436 - hutH OKGIIK_05610 0.29 +0.4 1,237,436 - hutH OKGIIK_05610 0.29 -1.6 1,237,436 - hutH OKGIIK_05610 0.29 +0.8 1,237,753 + hutH OKGIIK_05610 0.46 -0.9 1,237,753 + hutH OKGIIK_05610 0.46 -1.6 1,237,754 - hutH OKGIIK_05610 0.46 +0.9 1,237,754 - hutH OKGIIK_05610 0.46 -0.3 1,237,882 + hutH OKGIIK_05610 0.53 -0.5 1,237,882 + hutH OKGIIK_05610 0.53 -0.1 1,237,882 + hutH OKGIIK_05610 0.53 +0.8 1,237,882 + hutH OKGIIK_05610 0.53 +0.9 1,237,882 + hutH OKGIIK_05610 0.53 -0.6 1,237,882 + hutH OKGIIK_05610 0.53 -0.1 1,237,883 - hutH OKGIIK_05610 0.53 +0.4 1,237,883 - hutH OKGIIK_05610 0.53 -0.2 1,237,883 - hutH OKGIIK_05610 0.53 +0.7 1,237,936 + hutH OKGIIK_05610 0.55 -1.1 1,237,936 + hutH OKGIIK_05610 0.55 -2.0 1,237,936 + hutH OKGIIK_05610 0.55 +0.9 1,237,936 + hutH OKGIIK_05610 0.55 -0.5 1,237,936 + hutH OKGIIK_05610 0.55 -0.8 1,237,936 + hutH OKGIIK_05610 0.55 +1.1 1,237,936 + hutH OKGIIK_05610 0.55 -1.5 1,237,936 + hutH OKGIIK_05610 0.55 +1.3 1,237,936 + hutH OKGIIK_05610 0.55 +0.9 1,237,936 + hutH OKGIIK_05610 0.55 +0.3 1,237,936 + hutH OKGIIK_05610 0.55 -0.1 1,237,937 - hutH OKGIIK_05610 0.55 -1.6 1,237,937 - hutH OKGIIK_05610 0.55 -0.5 1,237,937 - hutH OKGIIK_05610 0.55 +0.5 1,237,937 - hutH OKGIIK_05610 0.55 +0.3 1,238,137 + hutH OKGIIK_05610 0.66 -1.5 1,238,137 + hutH OKGIIK_05610 0.66 +0.5 1,238,137 + hutH OKGIIK_05610 0.66 +0.7 1,238,137 + hutH OKGIIK_05610 0.66 +1.3 1,238,137 + hutH OKGIIK_05610 0.66 +0.1 1,238,137 + hutH OKGIIK_05610 0.66 -0.1 1,238,137 + hutH OKGIIK_05610 0.66 -0.8 1,238,137 + hutH OKGIIK_05610 0.66 -0.3 1,238,137 + hutH OKGIIK_05610 0.66 +1.0 1,238,137 + hutH OKGIIK_05610 0.66 +0.7 1,238,137 + hutH OKGIIK_05610 0.66 +0.2 1,238,137 + hutH OKGIIK_05610 0.66 +0.5 1,238,137 + hutH OKGIIK_05610 0.66 -1.3 1,238,137 + hutH OKGIIK_05610 0.66 -1.5 1,238,137 + hutH OKGIIK_05610 0.66 -0.9 1,238,138 - hutH OKGIIK_05610 0.66 +1.3 1,238,138 - hutH OKGIIK_05610 0.66 +0.9 1,238,138 - hutH OKGIIK_05610 0.66 +0.1 1,238,138 - hutH OKGIIK_05610 0.66 -1.8 1,238,138 - hutH OKGIIK_05610 0.66 -0.6 1,238,138 - hutH OKGIIK_05610 0.66 -2.2 1,238,138 - hutH OKGIIK_05610 0.66 -0.3 1,238,138 - hutH OKGIIK_05610 0.66 +0.4 1,238,138 - hutH OKGIIK_05610 0.66 -0.5 1,238,138 - hutH OKGIIK_05610 0.66 +1.4 1,238,138 - hutH OKGIIK_05610 0.66 +0.4 1,238,138 - hutH OKGIIK_05610 0.66 -0.2 1,238,138 - hutH OKGIIK_05610 0.66 +0.3 1,238,281 + hutH OKGIIK_05610 0.74 +1.7 1,238,281 + hutH OKGIIK_05610 0.74 -0.6 1,238,281 + hutH OKGIIK_05610 0.74 -0.9 1,238,281 + hutH OKGIIK_05610 0.74 -0.3 1,238,281 + hutH OKGIIK_05610 0.74 +0.7 1,238,281 + hutH OKGIIK_05610 0.74 -0.9 1,238,281 + hutH OKGIIK_05610 0.74 -1.3 1,238,282 - hutH OKGIIK_05610 0.74 +1.4 1,238,282 - hutH OKGIIK_05610 0.74 -0.9 1,238,282 - hutH OKGIIK_05610 0.74 +0.8 1,238,282 - hutH OKGIIK_05610 0.74 +0.4 1,238,282 - hutH OKGIIK_05610 0.74 -1.1 1,238,282 - hutH OKGIIK_05610 0.74 -2.1 1,238,282 - hutH OKGIIK_05610 0.74 -1.1 1,238,282 - hutH OKGIIK_05610 0.74 -0.1 1,238,282 - hutH OKGIIK_05610 0.74 +0.8 1,238,338 + hutH OKGIIK_05610 0.76 +0.6 1,238,338 + hutH OKGIIK_05610 0.76 -0.4 1,238,338 + hutH OKGIIK_05610 0.76 -0.0 1,238,338 + hutH OKGIIK_05610 0.76 -0.5 1,238,338 + hutH OKGIIK_05610 0.76 -0.3 1,238,338 + hutH OKGIIK_05610 0.76 -1.1 1,238,338 + hutH OKGIIK_05610 0.76 -0.9 1,238,339 - hutH OKGIIK_05610 0.77 -1.1 1,238,339 - hutH OKGIIK_05610 0.77 -0.1 1,238,339 - hutH OKGIIK_05610 0.77 +0.5 1,238,339 - hutH OKGIIK_05610 0.77 +0.4 1,238,339 - hutH OKGIIK_05610 0.77 -0.7 1,238,339 - hutH OKGIIK_05610 0.77 +1.0 1,238,339 - hutH OKGIIK_05610 0.77 -1.8 1,238,452 + hutH OKGIIK_05610 0.82 +1.7 1,238,452 + hutH OKGIIK_05610 0.82 +0.5 1,238,452 + hutH OKGIIK_05610 0.82 -0.4 1,238,452 + hutH OKGIIK_05610 0.82 +0.8 1,238,452 + hutH OKGIIK_05610 0.82 +1.0 1,238,452 + hutH OKGIIK_05610 0.82 -1.5 1,238,452 + hutH OKGIIK_05610 0.82 -1.4 1,238,453 - hutH OKGIIK_05610 0.83 +0.4 1,238,453 - hutH OKGIIK_05610 0.83 -0.1 1,238,453 - hutH OKGIIK_05610 0.83 +0.5 1,238,453 - hutH OKGIIK_05610 0.83 -0.3 1,238,453 - hutH OKGIIK_05610 0.83 +0.7 1,238,453 - hutH OKGIIK_05610 0.83 +0.9 1,238,582 - hutH OKGIIK_05610 0.89 -0.6 1,239,107 + OKGIIK_05615 0.74 -1.8 1,239,107 + OKGIIK_05615 0.74 -1.8 1,239,107 + OKGIIK_05615 0.74 +0.1 1,239,107 + OKGIIK_05615 0.74 -1.1 1,239,107 + OKGIIK_05615 0.74 -1.4 1,239,107 + OKGIIK_05615 0.74 -1.9 1,239,107 + OKGIIK_05615 0.74 +0.3 1,239,108 - OKGIIK_05615 0.74 -1.9 1,239,108 - OKGIIK_05615 0.74 -0.9 1,239,108 - OKGIIK_05615 0.74 -1.3 1,239,108 - OKGIIK_05615 0.74 -3.0 1,239,108 - OKGIIK_05615 0.74 -2.1 1,239,108 - OKGIIK_05615 0.74 -0.2 1,239,198 - -0.6 1,239,223 - +0.9 1,239,295 + -1.5 1,239,295 + +0.9 1,239,295 + -0.1 1,239,295 + -0.3 1,239,295 + +1.0 1,239,295 + -1.3 1,239,295 + -0.5 1,239,295 + -0.1 1,239,328 + -0.9 1,239,328 + -0.9 1,239,445 + rfaF OKGIIK_05620 0.14 +0.7 1,239,445 + rfaF OKGIIK_05620 0.14 -0.9 1,239,445 + rfaF OKGIIK_05620 0.14 -1.8 1,239,445 + rfaF OKGIIK_05620 0.14 -1.1 1,239,445 + rfaF OKGIIK_05620 0.14 -1.8 1,239,445 + rfaF OKGIIK_05620 0.14 -0.9 1,239,446 - rfaF OKGIIK_05620 0.14 -0.3 1,239,446 - rfaF OKGIIK_05620 0.14 -1.3 1,239,446 - rfaF OKGIIK_05620 0.14 +0.2 1,239,446 - rfaF OKGIIK_05620 0.14 -2.0 1,239,446 - rfaF OKGIIK_05620 0.14 -1.3 1,239,446 - rfaF OKGIIK_05620 0.14 -0.7 1,239,446 - rfaF OKGIIK_05620 0.14 -1.3 1,239,475 + rfaF OKGIIK_05620 0.17 -1.0 1,239,475 + rfaF OKGIIK_05620 0.17 -1.1 1,239,476 - rfaF OKGIIK_05620 0.17 -0.7 1,239,476 - rfaF OKGIIK_05620 0.17 -1.8 1,239,757 + rfaF OKGIIK_05620 0.44 -1.4 1,239,757 + rfaF OKGIIK_05620 0.44 -1.4 1,239,758 - rfaF OKGIIK_05620 0.44 -1.7
Or see this region's nucleotide sequence