Experiment: R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt csrA and Ser_trna are separated by 87 nucleotides Ser_trna and OKGIIK_03765 are separated by 380 nucleotides OKGIIK_03765 and OKGIIK_03770 are separated by 8 nucleotides OKGIIK_03770 and ppx are separated by 66 nucleotides
OKGIIK_03755: csrA - carbon storage regulator CsrA, at 829,685 to 829,888
csrA
OKGIIK_03760: Ser_trna - tRNA-Ser, at 829,976 to 830,068
_trna
OKGIIK_03765: OKGIIK_03765 - UDP-2,3-diacylglucosamine diphosphatase, at 830,449 to 831,177
_03765
OKGIIK_03770: OKGIIK_03770 - Peptidyl-prolyl cis-trans isomerase, at 831,186 to 831,887
_03770
OKGIIK_03775: ppx - exopolyphosphatase, at 831,954 to 833,471
ppx
Position (kb) 830
831
832 Strain fitness (log2 ratio) -2
-1
0
1
2 at 829.455 kb on - strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.488 kb on + strand at 829.491 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.670 kb on + strand at 829.909 kb on + strand at 829.909 kb on + strand at 829.909 kb on + strand at 830.072 kb on - strand at 830.072 kb on - strand at 830.072 kb on - strand at 830.072 kb on - strand at 830.072 kb on - strand at 830.103 kb on + strand at 830.104 kb on - strand at 830.104 kb on - strand at 830.104 kb on - strand at 830.247 kb on + strand at 830.268 kb on + strand at 830.268 kb on + strand at 830.268 kb on + strand at 830.268 kb on + strand at 830.268 kb on + strand at 830.268 kb on + strand at 830.268 kb on + strand at 830.268 kb on + strand at 830.269 kb on - strand at 830.269 kb on - strand at 830.269 kb on - strand at 830.269 kb on - strand at 830.297 kb on + strand at 830.297 kb on + strand at 830.297 kb on + strand at 830.297 kb on + strand at 830.298 kb on - strand at 830.415 kb on + strand at 831.623 kb on - strand, within OKGIIK_03770 at 831.698 kb on - strand, within OKGIIK_03770 at 831.698 kb on - strand, within OKGIIK_03770 at 831.740 kb on - strand, within OKGIIK_03770 at 831.740 kb on - strand, within OKGIIK_03770 at 831.921 kb on + strand at 831.921 kb on + strand at 831.921 kb on + strand at 831.921 kb on + strand at 831.921 kb on + strand at 831.922 kb on - strand at 831.922 kb on - strand at 831.968 kb on + strand at 831.968 kb on + strand at 831.969 kb on - strand at 832.168 kb on + strand, within ppx at 832.168 kb on + strand, within ppx at 832.169 kb on - strand, within ppx at 832.169 kb on - strand, within ppx
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM remove 829,455 - +0.6 829,488 + +0.5 829,488 + +0.7 829,488 + +0.9 829,488 + -0.9 829,488 + -0.0 829,488 + +0.8 829,488 + -1.2 829,488 + +2.1 829,488 + +2.2 829,488 + -0.4 829,488 + -1.1 829,488 + -0.2 829,488 + -0.9 829,488 + -1.2 829,488 + -0.1 829,488 + +0.7 829,488 + +1.2 829,488 + +0.7 829,488 + +0.9 829,488 + +0.2 829,488 + +0.8 829,491 + +0.7 829,670 + -1.8 829,670 + +0.2 829,670 + +1.1 829,670 + +1.1 829,670 + -0.2 829,670 + -1.3 829,670 + +0.3 829,670 + -1.7 829,670 + -0.6 829,670 + +2.1 829,670 + -0.9 829,670 + -1.7 829,670 + -1.5 829,909 + -1.5 829,909 + +1.6 829,909 + -2.3 830,072 - +0.4 830,072 - -0.6 830,072 - +1.0 830,072 - +1.6 830,072 - +1.9 830,103 + +0.1 830,104 - -0.1 830,104 - -0.3 830,104 - -0.1 830,247 + +1.2 830,268 + -0.8 830,268 + +0.4 830,268 + +1.2 830,268 + -0.5 830,268 + +0.6 830,268 + +0.6 830,268 + +0.5 830,268 + -0.9 830,269 - -0.4 830,269 - +0.6 830,269 - -0.1 830,269 - -1.8 830,297 + +0.0 830,297 + -0.7 830,297 + +0.5 830,297 + -0.9 830,298 - -0.6 830,415 + +1.0 831,623 - OKGIIK_03770 0.62 +0.4 831,698 - OKGIIK_03770 0.73 +1.6 831,698 - OKGIIK_03770 0.73 +0.6 831,740 - OKGIIK_03770 0.79 -1.7 831,740 - OKGIIK_03770 0.79 -1.7 831,921 + -0.9 831,921 + -1.4 831,921 + -0.0 831,921 + -0.4 831,921 + +0.1 831,922 - -0.9 831,922 - +0.1 831,968 + -0.3 831,968 + -1.5 831,969 - +0.0 832,168 + ppx OKGIIK_03775 0.14 +0.1 832,168 + ppx OKGIIK_03775 0.14 -0.3 832,169 - ppx OKGIIK_03775 0.14 +1.6 832,169 - ppx OKGIIK_03775 0.14 +0.3
Or see this region's nucleotide sequence