Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28320

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnhaP2 and ldcA are separated by 95 nucleotidesldcA and emtA are separated by 99 nucleotidesemtA and tnp-IS3 are separated by 187 nucleotides OKFHMN_28315: nhaP2 - potassium/proton antiporter, at 5,430,479 to 5,432,215 nhaP2 OKFHMN_28320: ldcA - muramoyltetrapeptide carboxypeptidase, at 5,432,311 to 5,433,225 ldcA OKFHMN_28325: emtA - membrane-bound lytic murein transglycosylase EmtA, at 5,433,325 to 5,433,936 emtA OKFHMN_28330: tnp-IS3 - IS3 family transposase, at 5,434,124 to 5,435,011 tnp-IS3 Position (kb) 5432 5433 5434Strain fitness (log2 ratio) -1 0 1 2at 5431.913 kb on - strand, within nhaP2at 5432.025 kb on - strand, within nhaP2at 5432.048 kb on - strandat 5432.117 kb on - strandat 5432.251 kb on - strandat 5433.258 kb on + strandat 5433.259 kb on - strandat 5433.311 kb on + strandat 5433.359 kb on + strandat 5433.390 kb on + strand, within emtAat 5433.505 kb on - strand, within emtAat 5433.660 kb on + strand, within emtAat 5433.669 kb on + strand, within emtAat 5433.742 kb on + strand, within emtA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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5,431,913 - nhaP2 OKFHMN_28315 0.83 +0.5
5,432,025 - nhaP2 OKFHMN_28315 0.89 -0.7
5,432,048 - +0.1
5,432,117 - -0.1
5,432,251 - -0.4
5,433,258 + +0.5
5,433,259 - +1.1
5,433,311 + -1.7
5,433,359 + +1.0
5,433,390 + emtA OKFHMN_28325 0.11 +0.8
5,433,505 - emtA OKFHMN_28325 0.29 -0.2
5,433,660 + emtA OKFHMN_28325 0.55 +2.0
5,433,669 + emtA OKFHMN_28325 0.56 +0.9
5,433,742 + emtA OKFHMN_28325 0.68 -0.8

Or see this region's nucleotide sequence