Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27505

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttyrS and pdxY are separated by 61 nucleotidespdxY and gstA are separated by 43 nucleotidesgstA and dtpA are separated by 105 nucleotides OKFHMN_27500: tyrS - tyrosine--tRNA ligase, at 5,293,471 to 5,294,745 tyrS OKFHMN_27505: pdxY - pyridoxal kinase PdxY, at 5,294,807 to 5,295,667 pdxY OKFHMN_27510: gstA - glutathione transferase GstA, at 5,295,711 to 5,296,316 gstA OKFHMN_27515: dtpA - dipeptide/tripeptide permease DtpA, at 5,296,422 to 5,297,924 dtpA Position (kb) 5294 5295 5296Strain fitness (log2 ratio) -2 -1 0 1at 5294.743 kb on + strandat 5296.424 kb on - strandat 5296.619 kb on - strand, within dtpA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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5,294,743 + -1.8
5,296,424 - -1.3
5,296,619 - dtpA OKFHMN_27515 0.13 -1.0

Or see this region's nucleotide sequence