Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25095

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_25085 and OKFHMN_25090 overlap by 4 nucleotidesOKFHMN_25090 and tnp-IS3 are separated by 177 nucleotidestnp-IS3 and tnp-IS3 overlap by 1 nucleotidestnp-IS3 and OKFHMN_25105 are separated by 51 nucleotides OKFHMN_25085: OKFHMN_25085 - chemotaxis protein, at 4,868,169 to 4,869,074 _25085 OKFHMN_25090: OKFHMN_25090 - dGTPase, at 4,869,071 to 4,869,754 _25090 OKFHMN_25095: tnp-IS3 - IS3 family transposase, at 4,869,932 to 4,870,819 tnp-IS3 OKFHMN_25100: tnp-IS3 - IS3 family transposase, at 4,870,819 to 4,871,145 tnp-IS3 OKFHMN_25105: OKFHMN_25105 - TonB-dependent receptor plug domain-containing protein, at 4,871,197 to 4,871,772 _25105 Position (kb) 4869 4870 4871Strain fitness (log2 ratio) -1 0 1at 4869.826 kb on - strandat 4871.321 kb on + strand, within OKFHMN_25105at 4871.321 kb on + strand, within OKFHMN_25105at 4871.377 kb on + strand, within OKFHMN_25105at 4871.423 kb on - strand, within OKFHMN_25105at 4871.423 kb on - strand, within OKFHMN_25105at 4871.512 kb on + strand, within OKFHMN_25105at 4871.542 kb on - strand, within OKFHMN_25105at 4871.707 kb on + strand, within OKFHMN_25105at 4871.758 kb on + strandat 4871.760 kb on + strandat 4871.760 kb on + strandat 4871.763 kb on + strandat 4871.788 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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4,869,826 - -1.7
4,871,321 + OKFHMN_25105 0.22 +0.6
4,871,321 + OKFHMN_25105 0.22 +1.6
4,871,377 + OKFHMN_25105 0.31 +0.3
4,871,423 - OKFHMN_25105 0.39 +0.6
4,871,423 - OKFHMN_25105 0.39 -0.1
4,871,512 + OKFHMN_25105 0.55 +0.1
4,871,542 - OKFHMN_25105 0.60 +0.2
4,871,707 + OKFHMN_25105 0.89 -0.7
4,871,758 + -0.4
4,871,760 + -0.9
4,871,760 + +0.4
4,871,763 + -1.7
4,871,788 + -0.2

Or see this region's nucleotide sequence