Strain Fitness in Escherichia coli ECRC100 around OKFHMN_23255

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_23235 and OKFHMN_23240 are separated by 51 nucleotidesOKFHMN_23240 and napF are separated by 15 nucleotidesnapF and napD overlap by 11 nucleotidesnapD and napA overlap by 4 nucleotidesnapA and napG are separated by 6 nucleotidesnapG and napH overlap by 14 nucleotides OKFHMN_23235: OKFHMN_23235 - hypothetical protein, at 4,536,034 to 4,536,198 _23235 OKFHMN_23240: OKFHMN_23240 - Orf 5, at 4,536,250 to 4,536,291 _23240 OKFHMN_23245: napF - ferredoxin-type protein NapF, at 4,536,307 to 4,536,801 napF OKFHMN_23250: napD - chaperone NapD, at 4,536,791 to 4,537,054 napD OKFHMN_23255: napA - nitrate reductase catalytic subunit NapA, at 4,537,051 to 4,539,537 napA OKFHMN_23260: napG - ferredoxin-type protein NapG, at 4,539,544 to 4,540,239 napG OKFHMN_23265: napH - quinol dehydrogenase ferredoxin subunit NapH, at 4,540,226 to 4,541,089 napH Position (kb) 4537 4538 4539 4540Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4536.179 kb on - strand, within OKFHMN_23235at 4536.214 kb on + strandat 4536.245 kb on + strandat 4536.436 kb on - strand, within napFat 4536.439 kb on - strand, within napFat 4536.524 kb on - strand, within napFat 4536.555 kb on - strand, within napFat 4536.700 kb on + strand, within napFat 4536.714 kb on + strand, within napFat 4536.715 kb on - strand, within napFat 4536.800 kb on - strandat 4536.978 kb on + strand, within napDat 4537.075 kb on - strandat 4537.168 kb on - strandat 4537.303 kb on + strand, within napAat 4537.325 kb on - strand, within napAat 4537.372 kb on - strand, within napAat 4537.483 kb on - strand, within napAat 4537.506 kb on + strand, within napAat 4537.519 kb on + strand, within napAat 4537.520 kb on - strand, within napAat 4537.587 kb on + strand, within napAat 4537.601 kb on + strand, within napAat 4537.627 kb on - strand, within napAat 4537.858 kb on + strand, within napAat 4537.871 kb on - strand, within napAat 4537.873 kb on - strand, within napAat 4538.036 kb on - strand, within napAat 4538.056 kb on + strand, within napAat 4538.058 kb on + strand, within napAat 4538.058 kb on + strand, within napAat 4538.059 kb on - strand, within napAat 4538.348 kb on + strand, within napAat 4538.349 kb on - strand, within napAat 4538.476 kb on + strand, within napAat 4538.532 kb on + strand, within napAat 4538.654 kb on - strand, within napAat 4538.673 kb on + strand, within napAat 4538.674 kb on - strand, within napAat 4538.696 kb on + strand, within napAat 4538.920 kb on + strand, within napAat 4538.947 kb on + strand, within napAat 4538.947 kb on + strand, within napAat 4538.948 kb on - strand, within napAat 4539.040 kb on - strand, within napAat 4539.069 kb on + strand, within napAat 4539.101 kb on - strand, within napAat 4539.102 kb on + strand, within napAat 4539.102 kb on + strand, within napAat 4539.109 kb on + strand, within napAat 4539.127 kb on - strand, within napAat 4539.184 kb on + strand, within napAat 4539.184 kb on + strand, within napAat 4539.185 kb on - strand, within napAat 4539.422 kb on - strandat 4539.422 kb on - strandat 4539.422 kb on - strandat 4539.535 kb on + strandat 4539.536 kb on - strandat 4539.543 kb on + strandat 4539.543 kb on + strandat 4539.760 kb on + strand, within napGat 4539.761 kb on - strand, within napGat 4539.814 kb on + strand, within napGat 4539.938 kb on + strand, within napGat 4540.160 kb on + strand, within napGat 4540.386 kb on - strand, within napHat 4540.387 kb on + strand, within napH

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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4,536,179 - OKFHMN_23235 0.88 -1.2
4,536,214 + +0.3
4,536,245 + -0.4
4,536,436 - napF OKFHMN_23245 0.26 +1.0
4,536,439 - napF OKFHMN_23245 0.27 +1.5
4,536,524 - napF OKFHMN_23245 0.44 -0.6
4,536,555 - napF OKFHMN_23245 0.50 +0.0
4,536,700 + napF OKFHMN_23245 0.79 -1.2
4,536,714 + napF OKFHMN_23245 0.82 -0.6
4,536,715 - napF OKFHMN_23245 0.82 -0.5
4,536,800 - -0.0
4,536,978 + napD OKFHMN_23250 0.71 +0.2
4,537,075 - -0.7
4,537,168 - +0.4
4,537,303 + napA OKFHMN_23255 0.10 -1.7
4,537,325 - napA OKFHMN_23255 0.11 -1.4
4,537,372 - napA OKFHMN_23255 0.13 +2.6
4,537,483 - napA OKFHMN_23255 0.17 -0.4
4,537,506 + napA OKFHMN_23255 0.18 -0.8
4,537,519 + napA OKFHMN_23255 0.19 -0.6
4,537,520 - napA OKFHMN_23255 0.19 -0.4
4,537,587 + napA OKFHMN_23255 0.22 +0.4
4,537,601 + napA OKFHMN_23255 0.22 +0.1
4,537,627 - napA OKFHMN_23255 0.23 -1.4
4,537,858 + napA OKFHMN_23255 0.32 +0.8
4,537,871 - napA OKFHMN_23255 0.33 +0.2
4,537,873 - napA OKFHMN_23255 0.33 +0.3
4,538,036 - napA OKFHMN_23255 0.40 -2.0
4,538,056 + napA OKFHMN_23255 0.40 -0.0
4,538,058 + napA OKFHMN_23255 0.40 +1.9
4,538,058 + napA OKFHMN_23255 0.40 +1.4
4,538,059 - napA OKFHMN_23255 0.41 -0.7
4,538,348 + napA OKFHMN_23255 0.52 +0.6
4,538,349 - napA OKFHMN_23255 0.52 +0.5
4,538,476 + napA OKFHMN_23255 0.57 +0.2
4,538,532 + napA OKFHMN_23255 0.60 -0.4
4,538,654 - napA OKFHMN_23255 0.64 -1.4
4,538,673 + napA OKFHMN_23255 0.65 -0.2
4,538,674 - napA OKFHMN_23255 0.65 -0.2
4,538,696 + napA OKFHMN_23255 0.66 -0.4
4,538,920 + napA OKFHMN_23255 0.75 +1.6
4,538,947 + napA OKFHMN_23255 0.76 +0.0
4,538,947 + napA OKFHMN_23255 0.76 -0.1
4,538,948 - napA OKFHMN_23255 0.76 -0.6
4,539,040 - napA OKFHMN_23255 0.80 +3.2
4,539,069 + napA OKFHMN_23255 0.81 -0.6
4,539,101 - napA OKFHMN_23255 0.82 +3.2
4,539,102 + napA OKFHMN_23255 0.82 -0.4
4,539,102 + napA OKFHMN_23255 0.82 -0.8
4,539,109 + napA OKFHMN_23255 0.83 +2.2
4,539,127 - napA OKFHMN_23255 0.83 +1.0
4,539,184 + napA OKFHMN_23255 0.86 -0.2
4,539,184 + napA OKFHMN_23255 0.86 +0.1
4,539,185 - napA OKFHMN_23255 0.86 +0.4
4,539,422 - +1.8
4,539,422 - -0.6
4,539,422 - +0.1
4,539,535 + +0.6
4,539,536 - +0.0
4,539,543 + -1.0
4,539,543 + -0.2
4,539,760 + napG OKFHMN_23260 0.31 +1.2
4,539,761 - napG OKFHMN_23260 0.31 +2.3
4,539,814 + napG OKFHMN_23260 0.39 -0.3
4,539,938 + napG OKFHMN_23260 0.57 -0.9
4,540,160 + napG OKFHMN_23260 0.89 -0.1
4,540,386 - napH OKFHMN_23265 0.19 -0.4
4,540,387 + napH OKFHMN_23265 0.19 -0.5

Or see this region's nucleotide sequence