Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20595

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthycE and hycF are separated by 9 nucleotideshycF and hycG overlap by 1 nucleotideshycG and hycH overlap by 4 nucleotides OKFHMN_20590: hycE - formate hydrogenlyase subunit HycE, at 4,006,235 to 4,007,944 hycE OKFHMN_20595: hycF - formate hydrogenlyase subunit HycF, at 4,007,954 to 4,008,496 hycF OKFHMN_20600: hycG - formate hydrogenlyase subunit HycG, at 4,008,496 to 4,009,263 hycG OKFHMN_20605: hycH - formate hydrogenlyase assembly protein HycH, at 4,009,260 to 4,009,670 hycH Position (kb) 4007 4008 4009Strain fitness (log2 ratio) -2 -1 0 1 2at 4007.018 kb on - strand, within hycEat 4007.201 kb on - strand, within hycEat 4007.515 kb on + strand, within hycEat 4007.536 kb on + strand, within hycEat 4007.537 kb on - strand, within hycEat 4007.549 kb on + strand, within hycEat 4007.550 kb on - strand, within hycEat 4007.620 kb on + strand, within hycEat 4007.625 kb on - strand, within hycEat 4007.715 kb on - strand, within hycEat 4007.715 kb on - strand, within hycEat 4007.766 kb on - strand, within hycEat 4007.866 kb on + strandat 4007.942 kb on + strandat 4007.960 kb on - strandat 4008.364 kb on + strand, within hycFat 4008.478 kb on + strandat 4008.507 kb on - strandat 4008.741 kb on - strand, within hycGat 4008.830 kb on - strand, within hycGat 4008.830 kb on - strand, within hycGat 4008.916 kb on + strand, within hycGat 4008.916 kb on + strand, within hycGat 4008.917 kb on - strand, within hycGat 4008.917 kb on - strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.138 kb on + strand, within hycGat 4009.144 kb on - strand, within hycGat 4009.152 kb on - strand, within hycGat 4009.254 kb on + strandat 4009.347 kb on + strand, within hycHat 4009.348 kb on - strand, within hycHat 4009.350 kb on + strand, within hycHat 4009.494 kb on + strand, within hycHat 4009.494 kb on + strand, within hycH

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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4,007,018 - hycE OKFHMN_20590 0.46 -0.5
4,007,201 - hycE OKFHMN_20590 0.56 +0.6
4,007,515 + hycE OKFHMN_20590 0.75 -0.3
4,007,536 + hycE OKFHMN_20590 0.76 -0.4
4,007,537 - hycE OKFHMN_20590 0.76 +0.9
4,007,549 + hycE OKFHMN_20590 0.77 -0.4
4,007,550 - hycE OKFHMN_20590 0.77 -1.6
4,007,620 + hycE OKFHMN_20590 0.81 +2.7
4,007,625 - hycE OKFHMN_20590 0.81 -2.3
4,007,715 - hycE OKFHMN_20590 0.87 -2.1
4,007,715 - hycE OKFHMN_20590 0.87 -0.1
4,007,766 - hycE OKFHMN_20590 0.90 +1.0
4,007,866 + -2.1
4,007,942 + -0.7
4,007,960 - +0.0
4,008,364 + hycF OKFHMN_20595 0.76 -0.2
4,008,478 + +0.5
4,008,507 - -0.1
4,008,741 - hycG OKFHMN_20600 0.32 +0.8
4,008,830 - hycG OKFHMN_20600 0.43 +0.8
4,008,830 - hycG OKFHMN_20600 0.43 -0.6
4,008,916 + hycG OKFHMN_20600 0.55 +0.0
4,008,916 + hycG OKFHMN_20600 0.55 -0.0
4,008,917 - hycG OKFHMN_20600 0.55 +0.5
4,008,917 - hycG OKFHMN_20600 0.55 +0.3
4,009,138 + hycG OKFHMN_20600 0.84 +0.1
4,009,138 + hycG OKFHMN_20600 0.84 -0.1
4,009,138 + hycG OKFHMN_20600 0.84 +0.4
4,009,144 - hycG OKFHMN_20600 0.84 -0.1
4,009,152 - hycG OKFHMN_20600 0.85 +0.5
4,009,254 + -1.0
4,009,347 + hycH OKFHMN_20605 0.21 +0.2
4,009,348 - hycH OKFHMN_20605 0.21 +0.5
4,009,350 + hycH OKFHMN_20605 0.22 -0.1
4,009,494 + hycH OKFHMN_20605 0.57 -1.0
4,009,494 + hycH OKFHMN_20605 0.57 -0.2

Or see this region's nucleotide sequence