Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypC and hypB overlap by 10 nucleotides hypB and hypA are separated by 3 nucleotides hypA and hycA are separated by 211 nucleotides hycA and hycB are separated by 124 nucleotides
OKFHMN_20555: hypC - hydrogenase 3 maturation protein HypC, at 4,000,570 to 4,000,842
hypC
OKFHMN_20560: hypB - hydrogenase nickel incorporation protein HypB, at 4,000,833 to 4,001,705
hypB
OKFHMN_20565: hypA - hydrogenase maturation nickel metallochaperone HypA, at 4,001,709 to 4,002,059
hypA
OKFHMN_20570: hycA - formate hydrogenlyase regulator HycA, at 4,002,271 to 4,002,732
hycA
OKFHMN_20575: hycB - formate hydrogenlyase subunit HycB, at 4,002,857 to 4,003,468
hycB
Position (kb) 4001
4002
4003 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4000.754 kb on - strand, within hypC at 4001.147 kb on + strand, within hypB at 4001.331 kb on + strand, within hypB at 4001.441 kb on + strand, within hypB at 4001.527 kb on + strand, within hypB at 4001.668 kb on + strand at 4001.668 kb on + strand at 4001.699 kb on + strand at 4001.824 kb on + strand, within hypA at 4001.880 kb on - strand, within hypA at 4001.982 kb on + strand, within hypA at 4002.048 kb on + strand at 4002.076 kb on + strand at 4002.077 kb on - strand at 4002.077 kb on - strand at 4002.213 kb on - strand at 4002.276 kb on + strand at 4002.277 kb on - strand at 4002.277 kb on - strand at 4002.349 kb on + strand, within hycA at 4002.349 kb on + strand, within hycA at 4002.350 kb on - strand, within hycA at 4002.350 kb on - strand, within hycA at 4002.350 kb on - strand, within hycA at 4002.350 kb on - strand, within hycA at 4002.459 kb on + strand, within hycA at 4002.463 kb on + strand, within hycA at 4002.463 kb on + strand, within hycA at 4002.505 kb on + strand, within hycA at 4002.506 kb on - strand, within hycA at 4002.506 kb on - strand, within hycA at 4002.508 kb on + strand, within hycA at 4002.659 kb on + strand, within hycA at 4002.660 kb on - strand, within hycA at 4002.661 kb on + strand, within hycA at 4002.661 kb on + strand, within hycA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 4,000,754 - hypC OKFHMN_20555 0.67 -0.1 4,001,147 + hypB OKFHMN_20560 0.36 -0.1 4,001,331 + hypB OKFHMN_20560 0.57 -1.9 4,001,441 + hypB OKFHMN_20560 0.70 -0.6 4,001,527 + hypB OKFHMN_20560 0.79 +0.9 4,001,668 + +1.2 4,001,668 + +0.0 4,001,699 + -0.7 4,001,824 + hypA OKFHMN_20565 0.33 -0.3 4,001,880 - hypA OKFHMN_20565 0.49 +0.0 4,001,982 + hypA OKFHMN_20565 0.78 -0.6 4,002,048 + -1.0 4,002,076 + -0.0 4,002,077 - +0.3 4,002,077 - -0.5 4,002,213 - -1.0 4,002,276 + +0.4 4,002,277 - +0.1 4,002,277 - +0.8 4,002,349 + hycA OKFHMN_20570 0.17 +0.0 4,002,349 + hycA OKFHMN_20570 0.17 +0.9 4,002,350 - hycA OKFHMN_20570 0.17 +0.3 4,002,350 - hycA OKFHMN_20570 0.17 +1.8 4,002,350 - hycA OKFHMN_20570 0.17 +0.0 4,002,350 - hycA OKFHMN_20570 0.17 +0.0 4,002,459 + hycA OKFHMN_20570 0.41 +0.1 4,002,463 + hycA OKFHMN_20570 0.42 -0.3 4,002,463 + hycA OKFHMN_20570 0.42 +0.8 4,002,505 + hycA OKFHMN_20570 0.51 -0.5 4,002,506 - hycA OKFHMN_20570 0.51 -1.4 4,002,506 - hycA OKFHMN_20570 0.51 -1.6 4,002,508 + hycA OKFHMN_20570 0.51 -1.6 4,002,659 + hycA OKFHMN_20570 0.84 -0.2 4,002,660 - hycA OKFHMN_20570 0.84 +0.3 4,002,661 + hycA OKFHMN_20570 0.84 -1.0 4,002,661 + hycA OKFHMN_20570 0.84 +0.1
Or see this region's nucleotide sequence