Strain Fitness in Escherichia coli ECRC100 around OKFHMN_19025

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybC and hybD overlap by 1 nucleotideshybD and hybE overlap by 8 nucleotideshybE and hypA overlap by 8 nucleotideshypA and hybG are separated by 12 nucleotides OKFHMN_19020: hybC - hydrogenase 2 large subunit, at 3,693,053 to 3,694,756 hybC OKFHMN_19025: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 3,694,756 to 3,695,250 hybD OKFHMN_19030: hybE - hydrogenase-2 assembly chaperone, at 3,695,243 to 3,695,731 hybE OKFHMN_19035: hypA - hydrogenase maturation nickel metallochaperone HypA, at 3,695,724 to 3,696,065 hypA OKFHMN_19040: hybG - hydrogenase maturation factor HybG, at 3,696,078 to 3,696,326 hybG Position (kb) 3694 3695 3696Strain fitness (log2 ratio) -1 0 1 2at 3693.776 kb on + strand, within hybCat 3693.777 kb on - strand, within hybCat 3693.777 kb on - strand, within hybCat 3693.824 kb on + strand, within hybCat 3693.825 kb on - strand, within hybCat 3693.960 kb on - strand, within hybCat 3694.016 kb on + strand, within hybCat 3694.058 kb on + strand, within hybCat 3694.059 kb on - strand, within hybCat 3694.059 kb on - strand, within hybCat 3694.094 kb on - strand, within hybCat 3694.109 kb on + strand, within hybCat 3694.198 kb on + strand, within hybCat 3694.199 kb on - strand, within hybCat 3694.199 kb on - strand, within hybCat 3694.379 kb on + strand, within hybCat 3694.492 kb on + strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.539 kb on - strand, within hybCat 3694.579 kb on + strand, within hybCat 3694.625 kb on - strandat 3694.646 kb on - strandat 3694.666 kb on + strandat 3694.667 kb on - strandat 3694.667 kb on - strandat 3694.755 kb on - strandat 3694.761 kb on + strandat 3694.858 kb on + strand, within hybDat 3694.858 kb on + strand, within hybDat 3694.859 kb on - strand, within hybDat 3695.257 kb on - strandat 3695.289 kb on + strandat 3695.289 kb on + strandat 3695.555 kb on + strand, within hybEat 3695.556 kb on - strand, within hybEat 3695.908 kb on + strand, within hypAat 3695.909 kb on - strand, within hypAat 3695.912 kb on + strand, within hypAat 3695.912 kb on + strand, within hypAat 3695.919 kb on + strand, within hypAat 3695.921 kb on + strand, within hypAat 3695.922 kb on - strand, within hypAat 3696.074 kb on - strandat 3696.083 kb on + strandat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.148 kb on + strand, within hybGat 3696.149 kb on - strand, within hybGat 3696.194 kb on + strand, within hybGat 3696.194 kb on + strand, within hybG

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,693,776 + hybC OKFHMN_19020 0.42 -0.5
3,693,777 - hybC OKFHMN_19020 0.42 -0.5
3,693,777 - hybC OKFHMN_19020 0.42 -0.9
3,693,824 + hybC OKFHMN_19020 0.45 -0.0
3,693,825 - hybC OKFHMN_19020 0.45 +0.1
3,693,960 - hybC OKFHMN_19020 0.53 -0.1
3,694,016 + hybC OKFHMN_19020 0.57 +0.1
3,694,058 + hybC OKFHMN_19020 0.59 +0.4
3,694,059 - hybC OKFHMN_19020 0.59 -0.4
3,694,059 - hybC OKFHMN_19020 0.59 +1.9
3,694,094 - hybC OKFHMN_19020 0.61 +0.8
3,694,109 + hybC OKFHMN_19020 0.62 -0.2
3,694,198 + hybC OKFHMN_19020 0.67 +0.9
3,694,199 - hybC OKFHMN_19020 0.67 -0.4
3,694,199 - hybC OKFHMN_19020 0.67 -0.5
3,694,379 + hybC OKFHMN_19020 0.78 -0.4
3,694,492 + hybC OKFHMN_19020 0.84 -0.5
3,694,539 - hybC OKFHMN_19020 0.87 +0.2
3,694,539 - hybC OKFHMN_19020 0.87 +0.5
3,694,539 - hybC OKFHMN_19020 0.87 +0.2
3,694,539 - hybC OKFHMN_19020 0.87 +0.3
3,694,539 - hybC OKFHMN_19020 0.87 -0.0
3,694,579 + hybC OKFHMN_19020 0.90 -0.2
3,694,625 - +1.9
3,694,646 - -0.2
3,694,666 + -0.7
3,694,667 - +0.1
3,694,667 - -0.6
3,694,755 - -0.3
3,694,761 + -0.1
3,694,858 + hybD OKFHMN_19025 0.21 -0.3
3,694,858 + hybD OKFHMN_19025 0.21 -0.6
3,694,859 - hybD OKFHMN_19025 0.21 +0.3
3,695,257 - +0.3
3,695,289 + -0.1
3,695,289 + -0.4
3,695,555 + hybE OKFHMN_19030 0.64 -1.0
3,695,556 - hybE OKFHMN_19030 0.64 -1.2
3,695,908 + hypA OKFHMN_19035 0.54 +0.0
3,695,909 - hypA OKFHMN_19035 0.54 +0.5
3,695,912 + hypA OKFHMN_19035 0.55 -0.3
3,695,912 + hypA OKFHMN_19035 0.55 +0.1
3,695,919 + hypA OKFHMN_19035 0.57 -0.7
3,695,921 + hypA OKFHMN_19035 0.58 -0.3
3,695,922 - hypA OKFHMN_19035 0.58 -0.7
3,696,074 - -0.2
3,696,083 + +0.2
3,696,148 + hybG OKFHMN_19040 0.28 +1.1
3,696,148 + hybG OKFHMN_19040 0.28 +0.3
3,696,148 + hybG OKFHMN_19040 0.28 -0.2
3,696,148 + hybG OKFHMN_19040 0.28 +0.5
3,696,149 - hybG OKFHMN_19040 0.29 -0.6
3,696,194 + hybG OKFHMN_19040 0.47 -0.1
3,696,194 + hybG OKFHMN_19040 0.47 +0.1

Or see this region's nucleotide sequence