Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05620

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_05610 and OKFHMN_05615 are separated by 293 nucleotidesOKFHMN_05615 and OKFHMN_05620 overlap by 4 nucleotidesOKFHMN_05620 and OKFHMN_05625 are separated by 46 nucleotidesOKFHMN_05625 and OKFHMN_05630 are separated by 49 nucleotidesOKFHMN_05630 and OKFHMN_05635 overlap by 1 nucleotidesOKFHMN_05635 and yeeS are separated by 68 nucleotides OKFHMN_05610: OKFHMN_05610 - restriction endonuclease subunit M, at 959,357 to 960,199 _05610 OKFHMN_05615: OKFHMN_05615 - hypothetical protein, at 960,493 to 960,981 _05615 OKFHMN_05620: OKFHMN_05620 - toxin, at 960,978 to 961,355 _05620 OKFHMN_05625: OKFHMN_05625 - antitoxin, at 961,402 to 961,776 _05625 OKFHMN_05630: OKFHMN_05630 - Phage-related protein, at 961,826 to 961,939 _05630 OKFHMN_05635: OKFHMN_05635 - DUF987 domain-containing protein, at 961,939 to 962,160 _05635 OKFHMN_05640: yeeS - UPF0758 protein YeeS, at 962,229 to 962,705 yeeS Position (kb) 960 961 962Strain fitness (log2 ratio) -2 -1 0 1at 960.658 kb on + strand, within OKFHMN_05615at 960.712 kb on - strand, within OKFHMN_05615at 960.781 kb on - strand, within OKFHMN_05615at 961.148 kb on - strand, within OKFHMN_05620at 961.860 kb on + strand, within OKFHMN_05630at 961.860 kb on + strand, within OKFHMN_05630at 961.860 kb on + strand, within OKFHMN_05630at 961.860 kb on + strand, within OKFHMN_05630at 962.080 kb on - strand, within OKFHMN_05635at 962.168 kb on + strandat 962.187 kb on + strandat 962.187 kb on + strandat 962.337 kb on - strand, within yeeS

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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960,658 + OKFHMN_05615 0.34 -2.1
960,712 - OKFHMN_05615 0.45 -0.6
960,781 - OKFHMN_05615 0.59 -0.4
961,148 - OKFHMN_05620 0.45 -0.9
961,860 + OKFHMN_05630 0.30 +1.0
961,860 + OKFHMN_05630 0.30 +0.8
961,860 + OKFHMN_05630 0.30 -0.7
961,860 + OKFHMN_05630 0.30 +0.3
962,080 - OKFHMN_05635 0.64 +1.1
962,168 + +0.3
962,187 + -0.6
962,187 + -0.1
962,337 - yeeS OKFHMN_05640 0.23 -0.6

Or see this region's nucleotide sequence