Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03845

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcsgB and cgsD are separated by 753 nucleotidescgsD and cgsE are separated by 4 nucleotidescgsE and cgsF are separated by 24 nucleotidescgsF and cgsG are separated by 26 nucleotides OKFHMN_03835: csgB - curlin minor subunit CsgB, at 612,562 to 613,017 csgB OKFHMN_03845: cgsD - transcriptional regulator CsgD, at 613,771 to 614,421 cgsD OKFHMN_03850: cgsE - curli production assembly/transport protein CsgE, at 614,426 to 614,815 cgsE OKFHMN_03855: cgsF - curli production assembly/transport protein CsgF, at 614,840 to 615,256 cgsF OKFHMN_03860: cgsG - curli production assembly/transport protein CsgG, at 615,283 to 616,116 cgsG Position (kb) 613 614 615Strain fitness (log2 ratio) -1 0 1at 612.901 kb on - strand, within csgBat 613.176 kb on - strandat 614.206 kb on + strand, within cgsDat 614.527 kb on + strand, within cgsEat 614.601 kb on + strand, within cgsEat 615.139 kb on + strand, within cgsFat 615.254 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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612,901 - csgB OKFHMN_03835 0.74 +1.1
613,176 - -0.5
614,206 + cgsD OKFHMN_03845 0.67 +0.2
614,527 + cgsE OKFHMN_03850 0.26 +1.3
614,601 + cgsE OKFHMN_03850 0.45 +0.9
615,139 + cgsF OKFHMN_03855 0.72 +0.3
615,254 + +0.2

Or see this region's nucleotide sequence