Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKFHMN_01715 and OKFHMN_01720 overlap by 14 nucleotides OKFHMN_01720 and OKFHMN_01725 are separated by 4 nucleotides OKFHMN_01725 and hrpA are separated by 145 nucleotides hrpA and azoR are separated by 200 nucleotides azoR and ynbD are separated by 50 nucleotides
OKFHMN_01715: OKFHMN_01715 - hypothetical protein, at 246,941 to 247,372
_01715
OKFHMN_01720: OKFHMN_01720 - hypothetical protein, at 247,359 to 247,697
_01720
OKFHMN_01725: OKFHMN_01725 - hypothetical protein, at 247,702 to 248,127
_01725
OKFHMN_01730: hrpA - ATP-dependent RNA helicase HrpA, at 248,273 to 252,175
hrpA
OKFHMN_01735: azoR - FMN-dependent NADH-azoreductase, at 252,376 to 252,981
azoR
OKFHMN_01740: ynbD - Uncharacterized protein YnbD, at 253,032 to 254,348
ynbD
Position (kb) 248
249
250
251
252
253 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 248.175 kb on + strand at 248.542 kb on - strand at 248.598 kb on - strand at 249.121 kb on + strand, within hrpA at 249.545 kb on - strand, within hrpA at 250.388 kb on - strand, within hrpA at 251.091 kb on + strand, within hrpA at 251.091 kb on + strand, within hrpA at 251.452 kb on + strand, within hrpA at 252.149 kb on - strand at 252.359 kb on - strand at 252.736 kb on + strand, within azoR at 253.113 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 248,175 + +0.4 248,542 - +0.6 248,598 - +4.3 249,121 + hrpA OKFHMN_01730 0.22 -1.9 249,545 - hrpA OKFHMN_01730 0.33 +1.9 250,388 - hrpA OKFHMN_01730 0.54 +2.3 251,091 + hrpA OKFHMN_01730 0.72 +1.4 251,091 + hrpA OKFHMN_01730 0.72 +0.3 251,452 + hrpA OKFHMN_01730 0.81 -0.9 252,149 - -0.0 252,359 - +0.8 252,736 + azoR OKFHMN_01735 0.59 +1.4 253,113 - -0.1
Or see this region's nucleotide sequence