Strain Fitness in Escherichia coli ECRC100 around OKFHMN_00725

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_00720 and phoQ are separated by 600 nucleotidesphoQ and phoP overlap by 1 nucleotidesphoP and purB are separated by 168 nucleotides OKFHMN_00720: OKFHMN_00720 - hypothetical protein, at 45,609 to 45,890 _00720 OKFHMN_00725: phoQ - two-component system sensor histidine kinase PhoQ, at 46,491 to 47,951 phoQ OKFHMN_00730: phoP - two-component system response regulator PhoP, at 47,951 to 48,622 phoP OKFHMN_00735: purB - adenylosuccinate lyase, at 48,791 to 50,161 purB Position (kb) 46 47 48Strain fitness (log2 ratio) -1 0 1 2at 45.625 kb on + strandat 46.187 kb on + strandat 46.349 kb on + strandat 46.723 kb on + strand, within phoQat 46.724 kb on - strand, within phoQat 46.888 kb on + strand, within phoQat 47.088 kb on - strand, within phoQat 47.687 kb on - strand, within phoQat 48.048 kb on - strand, within phoPat 48.196 kb on + strand, within phoPat 48.200 kb on + strand, within phoPat 48.654 kb on - strandat 48.658 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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45,625 + -1.4
46,187 + +0.4
46,349 + -0.9
46,723 + phoQ OKFHMN_00725 0.16 +0.0
46,724 - phoQ OKFHMN_00725 0.16 -0.4
46,888 + phoQ OKFHMN_00725 0.27 +0.8
47,088 - phoQ OKFHMN_00725 0.41 +0.6
47,687 - phoQ OKFHMN_00725 0.82 -1.2
48,048 - phoP OKFHMN_00730 0.14 -0.4
48,196 + phoP OKFHMN_00730 0.36 -0.6
48,200 + phoP OKFHMN_00730 0.37 +0.4
48,654 - +1.0
48,658 + +2.0

Or see this region's nucleotide sequence