Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27800

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspeG and ynfB are separated by 34 nucleotidesynfB and ynfA are separated by 134 nucleotidesynfA and OKFHMN_27805 are separated by 205 nucleotidesOKFHMN_27805 and OKFHMN_27810 are separated by 500 nucleotidesOKFHMN_27810 and OKFHMN_27815 are separated by 34 nucleotides OKFHMN_27790: speG - spermidine N1-acetyltransferase, at 5,353,874 to 5,354,434 speG OKFHMN_27795: ynfB - DUF1283 domain-containing protein, at 5,354,469 to 5,354,810 ynfB OKFHMN_27800: ynfA - YnfA family protein, at 5,354,945 to 5,355,271 ynfA OKFHMN_27805: OKFHMN_27805 - Starvation-sensing protein, at 5,355,477 to 5,355,569 _27805 OKFHMN_27810: OKFHMN_27810 - Integrase, cryptic prophage CP-933P, at 5,356,070 to 5,356,225 _27810 OKFHMN_27815: OKFHMN_27815 - DNA-binding protein, at 5,356,260 to 5,356,496 _27815 Position (kb) 5354 5355 5356Strain fitness (log2 ratio) -1 0 1at 5354.081 kb on - strand, within speGat 5354.380 kb on - strandat 5354.562 kb on - strand, within ynfBat 5354.825 kb on - strandat 5354.835 kb on - strandat 5354.889 kb on - strandat 5354.900 kb on - strandat 5354.902 kb on + strandat 5354.914 kb on + strandat 5354.975 kb on - strandat 5355.091 kb on - strand, within ynfAat 5355.143 kb on + strand, within ynfAat 5355.985 kb on - strandat 5356.181 kb on - strand, within OKFHMN_27810

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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5,354,081 - speG OKFHMN_27790 0.37 -0.8
5,354,380 - -0.5
5,354,562 - ynfB OKFHMN_27795 0.27 -0.8
5,354,825 - +1.5
5,354,835 - -0.5
5,354,889 - +0.7
5,354,900 - +0.9
5,354,902 + +0.7
5,354,914 + +0.8
5,354,975 - +0.3
5,355,091 - ynfA OKFHMN_27800 0.45 -0.1
5,355,143 + ynfA OKFHMN_27800 0.61 -0.1
5,355,985 - +1.2
5,356,181 - OKFHMN_27810 0.71 -0.4

Or see this region's nucleotide sequence