Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27640

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydgA and manA are separated by 100 nucleotidesmanA and fumB are separated by 198 nucleotides OKFHMN_27635: ydgA - Protein YdgA, at 5,319,259 to 5,320,767 ydgA OKFHMN_27640: manA - mannose-6-phosphate isomerase, at 5,320,868 to 5,322,043 manA OKFHMN_27645: fumB - class I fumarate hydratase, at 5,322,242 to 5,323,888 fumB Position (kb) 5320 5321 5322 5323Strain fitness (log2 ratio) -2 -1 0 1at 5320.818 kb on - strandat 5320.837 kb on - strandat 5321.060 kb on - strand, within manAat 5322.132 kb on + strandat 5322.228 kb on + strandat 5322.328 kb on + strandat 5322.494 kb on + strand, within fumBat 5322.967 kb on + strand, within fumB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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5,320,818 - +0.8
5,320,837 - -1.8
5,321,060 - manA OKFHMN_27640 0.16 -1.1
5,322,132 + -0.1
5,322,228 + -1.3
5,322,328 + -0.1
5,322,494 + fumB OKFHMN_27645 0.15 +0.9
5,322,967 + fumB OKFHMN_27645 0.44 +0.3

Or see this region's nucleotide sequence