Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26230

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcmoB and cmoA overlap by 4 nucleotidescmoA and yecN are separated by 40 nucleotidesyecN and yecE are separated by 52 nucleotidesyecE and yecD overlap by 4 nucleotides OKFHMN_26220: cmoB - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 5,054,076 to 5,055,047 cmoB OKFHMN_26225: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 5,055,044 to 5,055,787 cmoA OKFHMN_26230: yecN - Inner membrane protein YecN, at 5,055,828 to 5,056,223 yecN OKFHMN_26235: yecE - UPF0759 protein YecE, at 5,056,276 to 5,057,094 yecE OKFHMN_26240: yecD - hydrolase, at 5,057,091 to 5,057,657 yecD Position (kb) 5055 5056 5057Strain fitness (log2 ratio) -2 -1 0 1at 5054.930 kb on + strand, within cmoBat 5055.437 kb on - strand, within cmoAat 5055.456 kb on - strand, within cmoAat 5055.545 kb on - strand, within cmoAat 5055.545 kb on - strand, within cmoAat 5055.647 kb on - strand, within cmoAat 5055.647 kb on - strand, within cmoAat 5055.861 kb on - strandat 5055.863 kb on - strandat 5055.999 kb on - strand, within yecNat 5056.065 kb on - strand, within yecNat 5056.224 kb on - strandat 5056.259 kb on + strandat 5056.265 kb on - strandat 5056.356 kb on + strandat 5056.356 kb on + strandat 5056.360 kb on + strand, within yecEat 5056.383 kb on + strand, within yecEat 5056.407 kb on - strand, within yecEat 5056.494 kb on + strand, within yecEat 5056.535 kb on - strand, within yecEat 5057.087 kb on + strandat 5057.121 kb on - strandat 5057.148 kb on - strand, within yecD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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5,054,930 + cmoB OKFHMN_26220 0.88 -0.4
5,055,437 - cmoA OKFHMN_26225 0.53 +0.0
5,055,456 - cmoA OKFHMN_26225 0.55 +0.8
5,055,545 - cmoA OKFHMN_26225 0.67 +1.6
5,055,545 - cmoA OKFHMN_26225 0.67 -1.9
5,055,647 - cmoA OKFHMN_26225 0.81 -0.1
5,055,647 - cmoA OKFHMN_26225 0.81 -0.4
5,055,861 - -1.1
5,055,863 - +1.3
5,055,999 - yecN OKFHMN_26230 0.43 -1.0
5,056,065 - yecN OKFHMN_26230 0.60 -0.5
5,056,224 - +0.0
5,056,259 + +1.2
5,056,265 - +1.6
5,056,356 + +0.6
5,056,356 + -0.4
5,056,360 + yecE OKFHMN_26235 0.10 +0.2
5,056,383 + yecE OKFHMN_26235 0.13 +0.8
5,056,407 - yecE OKFHMN_26235 0.16 -0.7
5,056,494 + yecE OKFHMN_26235 0.27 +1.0
5,056,535 - yecE OKFHMN_26235 0.32 -0.7
5,057,087 + +1.3
5,057,121 - -0.7
5,057,148 - yecD OKFHMN_26240 0.10 -1.2

Or see this region's nucleotide sequence