Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24800

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_24780 and OKFHMN_24785 overlap by 10 nucleotidesOKFHMN_24785 and Ile2_trna are separated by 313 nucleotidesIle2_trna and Arg_trna are separated by 1 nucleotidesArg_trna and Arg_trna are separated by 13 nucleotidesArg_trna and stxA2c are separated by 92 nucleotides OKFHMN_24780: OKFHMN_24780 - Serine/threonine-protein phosphatase, at 4,820,067 to 4,820,738 _24780 OKFHMN_24785: OKFHMN_24785 - antiterminator, at 4,820,729 to 4,821,217 _24785 OKFHMN_24790: Ile2_trna - tRNA-Ile2(cat), at 4,821,531 to 4,821,606 _trna OKFHMN_24795: Arg_trna - tRNA-Arg(tcg), at 4,821,608 to 4,821,684 _trna OKFHMN_24800: Arg_trna - tRNA-Arg(tct), at 4,821,698 to 4,821,774 _trna OKFHMN_24805: stxA2c - Shiga toxin Stx2c subunit A, at 4,821,867 to 4,822,826 stxA2c Position (kb) 4821 4822Strain fitness (log2 ratio) -1 0 1 2at 4820.845 kb on - strand, within OKFHMN_24785at 4820.851 kb on + strand, within OKFHMN_24785at 4820.988 kb on - strand, within OKFHMN_24785at 4821.026 kb on + strand, within OKFHMN_24785at 4821.030 kb on - strand, within OKFHMN_24785at 4821.030 kb on - strand, within OKFHMN_24785at 4821.210 kb on + strandat 4821.215 kb on + strandat 4821.320 kb on - strandat 4821.519 kb on + strandat 4821.865 kb on - strandat 4821.865 kb on - strandat 4821.987 kb on - strand, within stxA2cat 4822.022 kb on + strand, within stxA2cat 4822.224 kb on - strand, within stxA2cat 4822.225 kb on + strand, within stxA2cat 4822.225 kb on + strand, within stxA2cat 4822.273 kb on - strand, within stxA2cat 4822.376 kb on + strand, within stxA2cat 4822.376 kb on + strand, within stxA2cat 4822.377 kb on - strand, within stxA2cat 4822.751 kb on + strandat 4822.770 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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4,820,845 - OKFHMN_24785 0.24 +0.6
4,820,851 + OKFHMN_24785 0.25 +0.6
4,820,988 - OKFHMN_24785 0.53 -0.3
4,821,026 + OKFHMN_24785 0.61 +0.2
4,821,030 - OKFHMN_24785 0.62 +0.3
4,821,030 - OKFHMN_24785 0.62 -0.4
4,821,210 + -0.4
4,821,215 + +0.4
4,821,320 - +0.2
4,821,519 + +0.9
4,821,865 - +1.9
4,821,865 - -0.3
4,821,987 - stxA2c OKFHMN_24805 0.12 -1.3
4,822,022 + stxA2c OKFHMN_24805 0.16 -0.0
4,822,224 - stxA2c OKFHMN_24805 0.37 -1.3
4,822,225 + stxA2c OKFHMN_24805 0.37 -0.3
4,822,225 + stxA2c OKFHMN_24805 0.37 +0.7
4,822,273 - stxA2c OKFHMN_24805 0.42 +0.1
4,822,376 + stxA2c OKFHMN_24805 0.53 +0.6
4,822,376 + stxA2c OKFHMN_24805 0.53 +0.6
4,822,377 - stxA2c OKFHMN_24805 0.53 +0.6
4,822,751 + -0.4
4,822,770 - +0.6

Or see this region's nucleotide sequence