Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menF and menD are separated by 88 nucleotides menD and menH overlap by 4 nucleotides menH and menB are separated by 14 nucleotides
OKFHMN_22930: menF - isochorismate synthase MenF, at 4,463,513 to 4,464,808
menF
OKFHMN_22935: menD - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene- 1-carboxylic-acid synthase, at 4,464,897 to 4,466,567
menD
OKFHMN_22940: menH - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase, at 4,466,564 to 4,467,322
menH
OKFHMN_22945: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,467,337 to 4,468,194
menB
Position (kb) 4464
4465
4466
4467 Strain fitness (log2 ratio) -1
0
1
2
3 at 4463.959 kb on + strand, within menF at 4463.960 kb on - strand, within menF at 4464.186 kb on - strand, within menF at 4464.288 kb on + strand, within menF at 4464.385 kb on + strand, within menF at 4464.441 kb on + strand, within menF at 4464.580 kb on + strand, within menF at 4464.632 kb on + strand, within menF at 4464.633 kb on - strand, within menF at 4464.705 kb on + strand at 4464.705 kb on + strand at 4464.792 kb on + strand at 4464.793 kb on - strand at 4464.795 kb on + strand at 4464.809 kb on + strand at 4464.810 kb on - strand at 4464.835 kb on + strand at 4464.877 kb on + strand at 4464.905 kb on - strand at 4464.998 kb on + strand at 4465.295 kb on + strand, within menD at 4465.295 kb on + strand, within menD at 4465.484 kb on + strand, within menD at 4466.077 kb on + strand, within menD at 4466.077 kb on + strand, within menD at 4466.257 kb on + strand, within menD at 4466.257 kb on + strand, within menD at 4466.288 kb on - strand, within menD at 4466.554 kb on + strand at 4466.564 kb on - strand at 4466.604 kb on + strand at 4466.693 kb on + strand, within menH at 4466.693 kb on + strand, within menH at 4466.763 kb on + strand, within menH at 4466.764 kb on - strand, within menH at 4466.786 kb on + strand, within menH at 4467.148 kb on + strand, within menH at 4467.364 kb on + strand at 4467.529 kb on + strand, within menB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 4,463,959 + menF OKFHMN_22930 0.34 +0.5 4,463,960 - menF OKFHMN_22930 0.34 +0.9 4,464,186 - menF OKFHMN_22930 0.52 +0.2 4,464,288 + menF OKFHMN_22930 0.60 -0.4 4,464,385 + menF OKFHMN_22930 0.67 +0.4 4,464,441 + menF OKFHMN_22930 0.72 +0.3 4,464,580 + menF OKFHMN_22930 0.82 +0.7 4,464,632 + menF OKFHMN_22930 0.86 -0.3 4,464,633 - menF OKFHMN_22930 0.86 +0.3 4,464,705 + -0.6 4,464,705 + +0.9 4,464,792 + -0.5 4,464,793 - +0.0 4,464,795 + -0.6 4,464,809 + +0.0 4,464,810 - -1.3 4,464,835 + +0.7 4,464,877 + +1.1 4,464,905 - -0.3 4,464,998 + -0.0 4,465,295 + menD OKFHMN_22935 0.24 -0.6 4,465,295 + menD OKFHMN_22935 0.24 +0.1 4,465,484 + menD OKFHMN_22935 0.35 -1.0 4,466,077 + menD OKFHMN_22935 0.71 -0.5 4,466,077 + menD OKFHMN_22935 0.71 +0.9 4,466,257 + menD OKFHMN_22935 0.81 +0.1 4,466,257 + menD OKFHMN_22935 0.81 +0.9 4,466,288 - menD OKFHMN_22935 0.83 +0.1 4,466,554 + +0.2 4,466,564 - -1.7 4,466,604 + +0.3 4,466,693 + menH OKFHMN_22940 0.17 +2.3 4,466,693 + menH OKFHMN_22940 0.17 +3.3 4,466,763 + menH OKFHMN_22940 0.26 -0.0 4,466,764 - menH OKFHMN_22940 0.26 -1.3 4,466,786 + menH OKFHMN_22940 0.29 +1.7 4,467,148 + menH OKFHMN_22940 0.77 -0.5 4,467,364 + +0.3 4,467,529 + menB OKFHMN_22945 0.22 +2.1
Or see this region's nucleotide sequence