Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22635

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfcA and epmC overlap by 1 nucleotidesepmC and yfcL are separated by 33 nucleotidesyfcL and mnmC are separated by 120 nucleotides OKFHMN_22625: yfcA - putative membrane transporter protein YfcA, at 4,402,656 to 4,403,465 yfcA OKFHMN_22630: epmC - elongation factor P hydroxylase, at 4,403,465 to 4,404,013 epmC OKFHMN_22635: yfcL - Uncharacterized protein YfcL, at 4,404,047 to 4,404,325 yfcL OKFHMN_22640: mnmC - bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC, at 4,404,446 to 4,406,452 mnmC Position (kb) 4404 4405Strain fitness (log2 ratio) -2 -1 0 1 2at 4403.074 kb on - strand, within yfcAat 4403.218 kb on + strand, within yfcAat 4403.418 kb on + strandat 4403.418 kb on + strandat 4403.463 kb on + strandat 4403.464 kb on - strandat 4403.464 kb on - strandat 4403.473 kb on + strandat 4403.545 kb on + strand, within epmCat 4403.567 kb on + strand, within epmCat 4403.589 kb on + strand, within epmCat 4403.590 kb on - strand, within epmCat 4403.595 kb on - strand, within epmCat 4403.595 kb on - strand, within epmCat 4403.660 kb on - strand, within epmCat 4403.813 kb on - strand, within epmCat 4403.954 kb on + strand, within epmCat 4403.957 kb on + strand, within epmCat 4404.151 kb on + strand, within yfcLat 4404.151 kb on + strand, within yfcLat 4404.151 kb on + strand, within yfcLat 4404.152 kb on - strand, within yfcLat 4404.152 kb on - strand, within yfcLat 4404.287 kb on - strand, within yfcLat 4404.287 kb on - strand, within yfcLat 4404.324 kb on - strandat 4404.395 kb on + strandat 4404.396 kb on - strandat 4404.467 kb on - strandat 4404.520 kb on + strandat 4404.609 kb on - strandat 4404.659 kb on - strand, within mnmCat 4404.719 kb on - strand, within mnmCat 4404.723 kb on - strand, within mnmCat 4404.864 kb on + strand, within mnmCat 4404.865 kb on - strand, within mnmCat 4404.905 kb on - strand, within mnmCat 4404.943 kb on - strand, within mnmCat 4404.952 kb on - strand, within mnmCat 4404.998 kb on - strand, within mnmCat 4405.171 kb on - strand, within mnmCat 4405.258 kb on + strand, within mnmCat 4405.259 kb on - strand, within mnmCat 4405.259 kb on - strand, within mnmCat 4405.259 kb on - strand, within mnmC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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4,403,074 - yfcA OKFHMN_22625 0.52 +0.7
4,403,218 + yfcA OKFHMN_22625 0.69 +0.8
4,403,418 + -0.7
4,403,418 + -0.8
4,403,463 + -0.2
4,403,464 - +1.1
4,403,464 - -1.1
4,403,473 + -0.1
4,403,545 + epmC OKFHMN_22630 0.15 -1.1
4,403,567 + epmC OKFHMN_22630 0.19 +0.3
4,403,589 + epmC OKFHMN_22630 0.23 +0.1
4,403,590 - epmC OKFHMN_22630 0.23 +2.7
4,403,595 - epmC OKFHMN_22630 0.24 -0.0
4,403,595 - epmC OKFHMN_22630 0.24 +0.0
4,403,660 - epmC OKFHMN_22630 0.36 -0.9
4,403,813 - epmC OKFHMN_22630 0.63 +1.7
4,403,954 + epmC OKFHMN_22630 0.89 -0.0
4,403,957 + epmC OKFHMN_22630 0.90 +0.5
4,404,151 + yfcL OKFHMN_22635 0.37 -0.3
4,404,151 + yfcL OKFHMN_22635 0.37 -1.6
4,404,151 + yfcL OKFHMN_22635 0.37 +0.4
4,404,152 - yfcL OKFHMN_22635 0.38 -1.1
4,404,152 - yfcL OKFHMN_22635 0.38 +1.0
4,404,287 - yfcL OKFHMN_22635 0.86 +0.2
4,404,287 - yfcL OKFHMN_22635 0.86 -0.3
4,404,324 - +1.7
4,404,395 + +0.7
4,404,396 - -0.5
4,404,467 - -0.6
4,404,520 + -1.1
4,404,609 - -0.4
4,404,659 - mnmC OKFHMN_22640 0.11 +0.1
4,404,719 - mnmC OKFHMN_22640 0.14 -0.4
4,404,723 - mnmC OKFHMN_22640 0.14 +0.3
4,404,864 + mnmC OKFHMN_22640 0.21 +0.1
4,404,865 - mnmC OKFHMN_22640 0.21 +2.1
4,404,905 - mnmC OKFHMN_22640 0.23 +2.4
4,404,943 - mnmC OKFHMN_22640 0.25 +0.3
4,404,952 - mnmC OKFHMN_22640 0.25 -0.8
4,404,998 - mnmC OKFHMN_22640 0.28 +0.3
4,405,171 - mnmC OKFHMN_22640 0.36 -0.4
4,405,258 + mnmC OKFHMN_22640 0.40 +1.7
4,405,259 - mnmC OKFHMN_22640 0.41 -2.2
4,405,259 - mnmC OKFHMN_22640 0.41 -1.5
4,405,259 - mnmC OKFHMN_22640 0.41 -0.6

Or see this region's nucleotide sequence