Strain Fitness in Escherichia coli ECRC100 around OKFHMN_21735

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisS and yfgM are separated by 17 nucleotidesyfgM and bamB are separated by 10 nucleotides OKFHMN_21730: hisS - histidine--tRNA ligase, at 4,217,863 to 4,219,137 hisS OKFHMN_21735: yfgM - ancillary SecYEG translocon subunit YfgM, at 4,219,155 to 4,219,775 yfgM OKFHMN_21740: bamB - outer membrane protein assembly factor BamB, at 4,219,786 to 4,220,964 bamB Position (kb) 4219 4220Strain fitness (log2 ratio) -2 -1 0 1at 4219.106 kb on + strandat 4219.196 kb on + strandat 4219.406 kb on + strand, within yfgMat 4219.688 kb on + strand, within yfgMat 4219.874 kb on + strandat 4220.002 kb on + strand, within bamBat 4220.024 kb on + strand, within bamBat 4220.114 kb on + strand, within bamBat 4220.297 kb on + strand, within bamBat 4220.573 kb on - strand, within bamB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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4,219,106 + -0.5
4,219,196 + -1.2
4,219,406 + yfgM OKFHMN_21735 0.40 +0.8
4,219,688 + yfgM OKFHMN_21735 0.86 +1.5
4,219,874 + -0.4
4,220,002 + bamB OKFHMN_21740 0.18 -2.5
4,220,024 + bamB OKFHMN_21740 0.20 -2.0
4,220,114 + bamB OKFHMN_21740 0.28 -1.0
4,220,297 + bamB OKFHMN_21740 0.43 -2.7
4,220,573 - bamB OKFHMN_21740 0.67 -1.0

Or see this region's nucleotide sequence