Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20820

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntproW and proV overlap by 8 nucleotidesproV and nrdF are separated by 355 nucleotides OKFHMN_20815: proW - glycine betaine/L-proline ABC transporter permease ProW, at 4,045,373 to 4,046,437 proW OKFHMN_20820: proV - glycine betaine/L-proline ABC transporter ATP-binding protein ProV, at 4,046,430 to 4,047,632 proV OKFHMN_20825: nrdF - class 1b ribonucleoside-diphosphate reductase subunit beta, at 4,047,988 to 4,048,947 nrdF Position (kb) 4046 4047 4048Strain fitness (log2 ratio) -1 0 1 2at 4045.606 kb on + strand, within proWat 4045.634 kb on - strand, within proWat 4045.833 kb on - strand, within proWat 4045.833 kb on - strand, within proWat 4045.833 kb on - strand, within proWat 4045.833 kb on - strand, within proWat 4045.855 kb on - strand, within proWat 4045.997 kb on - strand, within proWat 4046.129 kb on - strand, within proWat 4046.162 kb on - strand, within proWat 4046.458 kb on - strandat 4046.497 kb on + strandat 4046.498 kb on - strandat 4046.498 kb on - strandat 4046.498 kb on - strandat 4046.498 kb on - strandat 4046.646 kb on - strand, within proVat 4046.682 kb on - strand, within proVat 4046.855 kb on - strand, within proVat 4046.943 kb on - strand, within proVat 4047.283 kb on - strand, within proVat 4047.421 kb on - strand, within proVat 4047.466 kb on - strand, within proVat 4047.561 kb on - strandat 4047.653 kb on - strandat 4047.792 kb on - strandat 4047.857 kb on + strandat 4047.857 kb on + strandat 4048.038 kb on - strandat 4048.170 kb on + strand, within nrdFat 4048.171 kb on - strand, within nrdFat 4048.241 kb on + strand, within nrdFat 4048.241 kb on + strand, within nrdFat 4048.242 kb on - strand, within nrdFat 4048.344 kb on + strand, within nrdFat 4048.352 kb on - strand, within nrdFat 4048.352 kb on - strand, within nrdFat 4048.524 kb on - strand, within nrdFat 4048.537 kb on - strand, within nrdF

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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4,045,606 + proW OKFHMN_20815 0.22 +1.6
4,045,634 - proW OKFHMN_20815 0.25 +1.1
4,045,833 - proW OKFHMN_20815 0.43 +1.3
4,045,833 - proW OKFHMN_20815 0.43 +0.5
4,045,833 - proW OKFHMN_20815 0.43 +1.0
4,045,833 - proW OKFHMN_20815 0.43 +0.8
4,045,855 - proW OKFHMN_20815 0.45 +0.8
4,045,997 - proW OKFHMN_20815 0.59 +1.5
4,046,129 - proW OKFHMN_20815 0.71 +0.6
4,046,162 - proW OKFHMN_20815 0.74 +1.0
4,046,458 - +0.8
4,046,497 + -1.6
4,046,498 - +1.0
4,046,498 - +1.6
4,046,498 - +1.5
4,046,498 - +0.7
4,046,646 - proV OKFHMN_20820 0.18 +1.0
4,046,682 - proV OKFHMN_20820 0.21 +0.8
4,046,855 - proV OKFHMN_20820 0.35 +1.6
4,046,943 - proV OKFHMN_20820 0.43 +0.7
4,047,283 - proV OKFHMN_20820 0.71 +1.4
4,047,421 - proV OKFHMN_20820 0.82 +0.5
4,047,466 - proV OKFHMN_20820 0.86 +0.3
4,047,561 - +0.6
4,047,653 - +0.8
4,047,792 - +0.8
4,047,857 + +0.3
4,047,857 + -0.9
4,048,038 - +1.9
4,048,170 + nrdF OKFHMN_20825 0.19 -0.9
4,048,171 - nrdF OKFHMN_20825 0.19 +0.2
4,048,241 + nrdF OKFHMN_20825 0.26 +0.1
4,048,241 + nrdF OKFHMN_20825 0.26 +1.2
4,048,242 - nrdF OKFHMN_20825 0.26 +0.2
4,048,344 + nrdF OKFHMN_20825 0.37 +1.0
4,048,352 - nrdF OKFHMN_20825 0.38 +0.1
4,048,352 - nrdF OKFHMN_20825 0.38 -0.3
4,048,524 - nrdF OKFHMN_20825 0.56 +0.8
4,048,537 - nrdF OKFHMN_20825 0.57 -0.1

Or see this region's nucleotide sequence