Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt 16S_rrna and Ile_trna are separated by 68 nucleotides Ile_trna and Ala_trna are separated by 42 nucleotides Ala_trna and 23S_rrna are separated by 183 nucleotides 23S_rrna and 5S_rrna are separated by 94 nucleotides 5S_rrna and Thr_trna are separated by 14 nucleotides Thr_trna and 5S_rrna are separated by 39 nucleotides 5S_rrna and yhdZ are separated by 230 nucleotides
OKFHMN_17580: 16S_rrna - 16S ribosomal RNA, at 3,412,621 to 3,414,162
_rrna
OKFHMN_17585: Ile_trna - tRNA-Ile(gat), at 3,414,231 to 3,414,307
_trna
OKFHMN_17590: Ala_trna - tRNA-Ala(tgc), at 3,414,350 to 3,414,425
_trna
OKFHMN_17600: 23S_rrna - 23S ribosomal RNA, at 3,414,609 to 3,417,511
_rrna
OKFHMN_17605: 5S_rrna - 5S ribosomal RNA, at 3,417,606 to 3,417,721
_rrna
OKFHMN_17610: Thr_trna - tRNA-Thr(ggt), at 3,417,736 to 3,417,811
_trna
OKFHMN_17615: 5S_rrna - 5S ribosomal RNA, at 3,417,851 to 3,417,966
_rrna
OKFHMN_17620: yhdZ - Uncharacterized amino-acid ABC transporter ATP-binding protein YhdZ, at 3,418,197 to 3,418,955
yhdZ
Position (kb)
3414
3415
3416
3417
3418 Strain fitness (log2 ratio)
-2
-1
0
1 at 3417.817 kb on + strand at 3417.817 kb on + strand at 3418.014 kb on - strand at 3418.014 kb on - strand at 3418.173 kb on + strand at 3418.188 kb on - strand at 3418.230 kb on - strand at 3418.242 kb on - strand at 3418.392 kb on - strand, within yhdZ at 3418.467 kb on - strand, within yhdZ
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 3,417,817 + -0.5 3,417,817 + +0.1 3,418,014 - -0.1 3,418,014 - -0.2 3,418,173 + +1.1 3,418,188 - +0.2 3,418,230 - -0.1 3,418,242 - -0.5 3,418,392 - yhdZ OKFHMN_17620 0.26 -2.1 3,418,467 - yhdZ OKFHMN_17620 0.36 +1.1
Or see this region's nucleotide sequence