Strain Fitness in Escherichia coli ECRC100 around OKFHMN_17185

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcysG and nirC are separated by 18 nucleotidesnirC and nirD are separated by 125 nucleotidesnirD and nirB overlap by 4 nucleotides OKFHMN_17180: cysG - siroheme synthase CysG, at 3,355,974 to 3,357,347 cysG OKFHMN_17185: nirC - nitrite transporter NirC, at 3,357,366 to 3,358,172 nirC OKFHMN_17190: nirD - nitrite reductase small subunit NirD, at 3,358,298 to 3,358,624 nirD OKFHMN_17195: nirB - NADPH-nitrite reductase large subunit, at 3,358,621 to 3,361,164 nirB Position (kb) 3357 3358 3359Strain fitness (log2 ratio) -2 -1 0 1at 3356.582 kb on + strand, within cysGat 3356.727 kb on + strand, within cysGat 3356.728 kb on - strand, within cysGat 3356.854 kb on - strand, within cysGat 3356.865 kb on + strand, within cysGat 3356.957 kb on + strand, within cysGat 3356.958 kb on - strand, within cysGat 3357.000 kb on - strand, within cysGat 3357.175 kb on - strand, within cysGat 3357.175 kb on - strand, within cysGat 3357.219 kb on + strandat 3357.220 kb on - strandat 3357.318 kb on + strandat 3357.319 kb on - strandat 3357.319 kb on - strandat 3357.330 kb on + strandat 3357.350 kb on - strandat 3357.364 kb on - strandat 3357.364 kb on - strandat 3357.364 kb on - strandat 3357.364 kb on - strandat 3357.428 kb on + strandat 3357.433 kb on + strandat 3357.434 kb on - strandat 3357.434 kb on - strandat 3357.434 kb on - strandat 3357.456 kb on + strand, within nirCat 3357.469 kb on - strand, within nirCat 3357.473 kb on + strand, within nirCat 3357.473 kb on + strand, within nirCat 3357.474 kb on - strand, within nirCat 3357.474 kb on - strand, within nirCat 3357.573 kb on - strand, within nirCat 3357.693 kb on - strand, within nirCat 3357.717 kb on + strand, within nirCat 3357.771 kb on - strand, within nirCat 3357.803 kb on + strand, within nirCat 3357.803 kb on + strand, within nirCat 3357.803 kb on + strand, within nirCat 3357.803 kb on + strand, within nirCat 3357.804 kb on - strand, within nirCat 3357.804 kb on - strand, within nirCat 3357.804 kb on - strand, within nirCat 3357.805 kb on + strand, within nirCat 3357.806 kb on - strand, within nirCat 3357.806 kb on - strand, within nirCat 3357.806 kb on - strand, within nirCat 3358.063 kb on + strand, within nirCat 3358.064 kb on - strand, within nirCat 3358.152 kb on - strandat 3358.155 kb on - strandat 3358.248 kb on + strandat 3358.341 kb on + strand, within nirDat 3358.342 kb on - strand, within nirDat 3358.556 kb on + strand, within nirDat 3358.557 kb on - strand, within nirDat 3358.645 kb on + strandat 3358.646 kb on - strandat 3358.654 kb on - strandat 3358.767 kb on + strandat 3358.768 kb on - strandat 3358.938 kb on + strand, within nirBat 3359.051 kb on + strand, within nirBat 3359.051 kb on + strand, within nirBat 3359.052 kb on - strand, within nirBat 3359.079 kb on + strand, within nirBat 3359.083 kb on - strand, within nirB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,356,582 + cysG OKFHMN_17180 0.44 -1.9
3,356,727 + cysG OKFHMN_17180 0.55 +0.5
3,356,728 - cysG OKFHMN_17180 0.55 -0.7
3,356,854 - cysG OKFHMN_17180 0.64 -0.8
3,356,865 + cysG OKFHMN_17180 0.65 +0.7
3,356,957 + cysG OKFHMN_17180 0.72 -0.9
3,356,958 - cysG OKFHMN_17180 0.72 -0.9
3,357,000 - cysG OKFHMN_17180 0.75 -0.3
3,357,175 - cysG OKFHMN_17180 0.87 -0.2
3,357,175 - cysG OKFHMN_17180 0.87 -0.7
3,357,219 + -1.6
3,357,220 - -0.7
3,357,318 + +0.5
3,357,319 - +0.2
3,357,319 - +0.6
3,357,330 + +1.4
3,357,350 - -0.4
3,357,364 - +0.2
3,357,364 - +0.9
3,357,364 - +0.7
3,357,364 - -0.7
3,357,428 + +0.9
3,357,433 + +0.2
3,357,434 - +0.0
3,357,434 - -0.6
3,357,434 - +0.2
3,357,456 + nirC OKFHMN_17185 0.11 -0.0
3,357,469 - nirC OKFHMN_17185 0.13 -0.4
3,357,473 + nirC OKFHMN_17185 0.13 -0.6
3,357,473 + nirC OKFHMN_17185 0.13 -0.7
3,357,474 - nirC OKFHMN_17185 0.13 +0.3
3,357,474 - nirC OKFHMN_17185 0.13 +0.2
3,357,573 - nirC OKFHMN_17185 0.26 -0.7
3,357,693 - nirC OKFHMN_17185 0.41 +0.3
3,357,717 + nirC OKFHMN_17185 0.43 +0.2
3,357,771 - nirC OKFHMN_17185 0.50 -0.9
3,357,803 + nirC OKFHMN_17185 0.54 -0.7
3,357,803 + nirC OKFHMN_17185 0.54 -0.7
3,357,803 + nirC OKFHMN_17185 0.54 -0.8
3,357,803 + nirC OKFHMN_17185 0.54 +0.1
3,357,804 - nirC OKFHMN_17185 0.54 -0.4
3,357,804 - nirC OKFHMN_17185 0.54 +0.0
3,357,804 - nirC OKFHMN_17185 0.54 +0.2
3,357,805 + nirC OKFHMN_17185 0.54 +1.7
3,357,806 - nirC OKFHMN_17185 0.55 -1.0
3,357,806 - nirC OKFHMN_17185 0.55 +0.3
3,357,806 - nirC OKFHMN_17185 0.55 +0.2
3,358,063 + nirC OKFHMN_17185 0.86 +0.7
3,358,064 - nirC OKFHMN_17185 0.86 -0.8
3,358,152 - +1.0
3,358,155 - -1.8
3,358,248 + -1.2
3,358,341 + nirD OKFHMN_17190 0.13 -1.5
3,358,342 - nirD OKFHMN_17190 0.13 -1.5
3,358,556 + nirD OKFHMN_17190 0.79 -1.7
3,358,557 - nirD OKFHMN_17190 0.79 -0.2
3,358,645 + +1.2
3,358,646 - +0.9
3,358,654 - -0.8
3,358,767 + -0.2
3,358,768 - -0.5
3,358,938 + nirB OKFHMN_17195 0.12 +0.5
3,359,051 + nirB OKFHMN_17195 0.17 -0.1
3,359,051 + nirB OKFHMN_17195 0.17 -0.5
3,359,052 - nirB OKFHMN_17195 0.17 +0.5
3,359,079 + nirB OKFHMN_17195 0.18 +0.4
3,359,083 - nirB OKFHMN_17195 0.18 -0.7

Or see this region's nucleotide sequence