Strain Fitness in Escherichia coli ECRC100 around OKFHMN_16535

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnikE and nikE are separated by 5 nucleotidesnikE and nikD overlap by 4 nucleotidesnikD and nikC overlap by 1 nucleotidesnikC and nikB overlap by 4 nucleotides OKFHMN_16525: nikE - nickel import ATP-binding protein NikE, at 3,221,490 to 3,221,891 nikE OKFHMN_16530: nikE - nickel import ATP-binding protein NikE, at 3,221,897 to 3,222,703 nikE OKFHMN_16535: nikD - nickel import ATP-binding protein NikD, at 3,222,700 to 3,223,464 nikD OKFHMN_16540: nikC - nickel ABC transporter permease subunit NikC, at 3,223,464 to 3,224,297 nikC OKFHMN_16545: nikB - nickel ABC transporter permease subunit NikB, at 3,224,294 to 3,225,238 nikB Position (kb) 3222 3223 3224Strain fitness (log2 ratio) -2 -1 0 1 2at 3221.713 kb on + strand, within nikEat 3221.713 kb on + strand, within nikEat 3221.719 kb on + strand, within nikEat 3221.720 kb on - strand, within nikEat 3221.720 kb on - strand, within nikEat 3221.816 kb on + strand, within nikEat 3221.816 kb on + strand, within nikEat 3221.817 kb on - strand, within nikEat 3221.856 kb on + strandat 3222.069 kb on - strand, within nikEat 3222.069 kb on - strand, within nikEat 3222.164 kb on - strand, within nikEat 3222.164 kb on - strand, within nikEat 3222.248 kb on + strand, within nikEat 3222.422 kb on + strand, within nikEat 3222.497 kb on - strand, within nikEat 3222.521 kb on + strand, within nikEat 3222.666 kb on + strandat 3222.692 kb on - strandat 3222.692 kb on - strandat 3222.695 kb on + strandat 3222.695 kb on + strandat 3222.695 kb on + strandat 3222.696 kb on - strandat 3222.723 kb on - strandat 3222.865 kb on - strand, within nikDat 3222.907 kb on - strand, within nikDat 3222.981 kb on + strand, within nikDat 3222.981 kb on + strand, within nikDat 3223.099 kb on + strand, within nikDat 3223.099 kb on + strand, within nikDat 3223.099 kb on + strand, within nikDat 3223.117 kb on - strand, within nikDat 3223.440 kb on + strandat 3223.440 kb on + strandat 3223.440 kb on + strandat 3223.441 kb on - strandat 3223.441 kb on - strandat 3223.444 kb on + strandat 3223.444 kb on + strandat 3223.445 kb on - strandat 3223.547 kb on + strandat 3223.595 kb on + strand, within nikCat 3223.595 kb on + strand, within nikCat 3223.596 kb on - strand, within nikCat 3223.692 kb on - strand, within nikCat 3223.884 kb on - strand, within nikCat 3223.887 kb on + strand, within nikCat 3223.888 kb on - strand, within nikCat 3223.888 kb on - strand, within nikCat 3223.923 kb on - strand, within nikCat 3224.198 kb on - strand, within nikCat 3224.365 kb on + strandat 3224.366 kb on - strandat 3224.399 kb on - strand, within nikBat 3224.399 kb on - strand, within nikB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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3,221,713 + nikE OKFHMN_16525 0.55 +1.2
3,221,713 + nikE OKFHMN_16525 0.55 -0.3
3,221,719 + nikE OKFHMN_16525 0.57 -0.6
3,221,720 - nikE OKFHMN_16525 0.57 -0.1
3,221,720 - nikE OKFHMN_16525 0.57 -0.9
3,221,816 + nikE OKFHMN_16525 0.81 -0.3
3,221,816 + nikE OKFHMN_16525 0.81 +0.4
3,221,817 - nikE OKFHMN_16525 0.81 +0.5
3,221,856 + +1.3
3,222,069 - nikE OKFHMN_16530 0.21 +0.1
3,222,069 - nikE OKFHMN_16530 0.21 -0.5
3,222,164 - nikE OKFHMN_16530 0.33 +0.3
3,222,164 - nikE OKFHMN_16530 0.33 -0.2
3,222,248 + nikE OKFHMN_16530 0.43 +0.4
3,222,422 + nikE OKFHMN_16530 0.65 -1.3
3,222,497 - nikE OKFHMN_16530 0.74 -0.4
3,222,521 + nikE OKFHMN_16530 0.77 -0.5
3,222,666 + -0.4
3,222,692 - -0.4
3,222,692 - +0.5
3,222,695 + -1.4
3,222,695 + +0.9
3,222,695 + -0.2
3,222,696 - +1.3
3,222,723 - +0.7
3,222,865 - nikD OKFHMN_16535 0.22 -1.0
3,222,907 - nikD OKFHMN_16535 0.27 -0.3
3,222,981 + nikD OKFHMN_16535 0.37 +0.9
3,222,981 + nikD OKFHMN_16535 0.37 +0.5
3,223,099 + nikD OKFHMN_16535 0.52 +0.0
3,223,099 + nikD OKFHMN_16535 0.52 +0.7
3,223,099 + nikD OKFHMN_16535 0.52 +0.5
3,223,117 - nikD OKFHMN_16535 0.55 -1.7
3,223,440 + -0.1
3,223,440 + +0.6
3,223,440 + -1.1
3,223,441 - +0.6
3,223,441 - +0.1
3,223,444 + -0.8
3,223,444 + +0.5
3,223,445 - +0.9
3,223,547 + +0.1
3,223,595 + nikC OKFHMN_16540 0.16 -0.3
3,223,595 + nikC OKFHMN_16540 0.16 +0.1
3,223,596 - nikC OKFHMN_16540 0.16 +0.3
3,223,692 - nikC OKFHMN_16540 0.27 -0.7
3,223,884 - nikC OKFHMN_16540 0.50 +0.0
3,223,887 + nikC OKFHMN_16540 0.51 +2.1
3,223,888 - nikC OKFHMN_16540 0.51 +0.3
3,223,888 - nikC OKFHMN_16540 0.51 +0.3
3,223,923 - nikC OKFHMN_16540 0.55 -0.3
3,224,198 - nikC OKFHMN_16540 0.88 -1.2
3,224,365 + -1.2
3,224,366 - -2.3
3,224,399 - nikB OKFHMN_16545 0.11 -0.7
3,224,399 - nikB OKFHMN_16545 0.11 +0.4

Or see this region's nucleotide sequence