Strain Fitness in Escherichia coli ECRC100 around OKFHMN_13395

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiE and thiF overlap by 8 nucleotidesthiF and thiS overlap by 17 nucleotidesthiS and thiG are separated by 1 nucleotidesthiG and thiH overlap by 4 nucleotides OKFHMN_13380: thiE - thiamine phosphate synthase, at 2,572,013 to 2,572,648 thiE OKFHMN_13385: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 2,572,641 to 2,573,396 thiF OKFHMN_13390: thiS - sulfur carrier protein ThiS, at 2,573,380 to 2,573,580 thiS OKFHMN_13395: thiG - thiazole synthase, at 2,573,582 to 2,574,352 thiG OKFHMN_13400: thiH - 2-iminoacetate synthase ThiH, at 2,574,349 to 2,575,482 thiH Position (kb) 2573 2574 2575Strain fitness (log2 ratio) -1 0 1 2at 2572.668 kb on - strandat 2572.760 kb on + strand, within thiFat 2572.774 kb on + strand, within thiFat 2572.776 kb on + strand, within thiFat 2572.777 kb on - strand, within thiFat 2572.835 kb on - strand, within thiFat 2572.875 kb on - strand, within thiFat 2572.948 kb on + strand, within thiFat 2572.948 kb on + strand, within thiFat 2572.948 kb on + strand, within thiFat 2572.948 kb on + strand, within thiFat 2572.949 kb on - strand, within thiFat 2572.949 kb on - strand, within thiFat 2572.949 kb on - strand, within thiFat 2572.976 kb on - strand, within thiFat 2573.024 kb on + strand, within thiFat 2573.045 kb on + strand, within thiFat 2573.045 kb on + strand, within thiFat 2573.258 kb on + strand, within thiFat 2573.259 kb on - strand, within thiFat 2573.259 kb on - strand, within thiFat 2573.264 kb on - strand, within thiFat 2573.312 kb on + strand, within thiFat 2573.488 kb on - strand, within thiSat 2573.526 kb on + strand, within thiSat 2573.526 kb on + strand, within thiSat 2573.526 kb on + strand, within thiSat 2573.527 kb on - strand, within thiSat 2573.587 kb on - strandat 2573.785 kb on + strand, within thiGat 2573.786 kb on - strand, within thiGat 2574.089 kb on - strand, within thiGat 2574.089 kb on - strand, within thiGat 2574.186 kb on + strand, within thiGat 2574.299 kb on + strandat 2574.431 kb on + strandat 2574.567 kb on + strand, within thiHat 2574.619 kb on + strand, within thiHat 2574.619 kb on + strand, within thiHat 2574.619 kb on + strand, within thiHat 2574.620 kb on - strand, within thiHat 2574.766 kb on + strand, within thiHat 2574.825 kb on - strand, within thiHat 2574.825 kb on - strand, within thiHat 2574.889 kb on + strand, within thiHat 2574.901 kb on + strand, within thiHat 2574.901 kb on + strand, within thiHat 2574.901 kb on + strand, within thiHat 2575.003 kb on - strand, within thiHat 2575.007 kb on + strand, within thiHat 2575.023 kb on - strand, within thiHat 2575.057 kb on + strand, within thiHat 2575.057 kb on + strand, within thiHat 2575.058 kb on - strand, within thiHat 2575.058 kb on - strand, within thiHat 2575.059 kb on + strand, within thiHat 2575.097 kb on - strand, within thiHat 2575.111 kb on + strand, within thiHat 2575.144 kb on - strand, within thiHat 2575.188 kb on - strand, within thiHat 2575.293 kb on + strand, within thiHat 2575.294 kb on - strand, within thiH

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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2,572,668 - +0.5
2,572,760 + thiF OKFHMN_13385 0.16 -1.1
2,572,774 + thiF OKFHMN_13385 0.18 +0.1
2,572,776 + thiF OKFHMN_13385 0.18 -0.7
2,572,777 - thiF OKFHMN_13385 0.18 -0.7
2,572,835 - thiF OKFHMN_13385 0.26 +0.3
2,572,875 - thiF OKFHMN_13385 0.31 +0.1
2,572,948 + thiF OKFHMN_13385 0.41 -1.4
2,572,948 + thiF OKFHMN_13385 0.41 -0.5
2,572,948 + thiF OKFHMN_13385 0.41 +0.4
2,572,948 + thiF OKFHMN_13385 0.41 +0.8
2,572,949 - thiF OKFHMN_13385 0.41 +0.7
2,572,949 - thiF OKFHMN_13385 0.41 +0.0
2,572,949 - thiF OKFHMN_13385 0.41 +0.2
2,572,976 - thiF OKFHMN_13385 0.44 +2.1
2,573,024 + thiF OKFHMN_13385 0.51 -0.4
2,573,045 + thiF OKFHMN_13385 0.53 -1.2
2,573,045 + thiF OKFHMN_13385 0.53 +0.4
2,573,258 + thiF OKFHMN_13385 0.82 +0.2
2,573,259 - thiF OKFHMN_13385 0.82 +0.1
2,573,259 - thiF OKFHMN_13385 0.82 +0.4
2,573,264 - thiF OKFHMN_13385 0.82 +2.1
2,573,312 + thiF OKFHMN_13385 0.89 +2.1
2,573,488 - thiS OKFHMN_13390 0.54 +0.2
2,573,526 + thiS OKFHMN_13390 0.73 -0.0
2,573,526 + thiS OKFHMN_13390 0.73 +0.5
2,573,526 + thiS OKFHMN_13390 0.73 +0.9
2,573,527 - thiS OKFHMN_13390 0.73 +0.3
2,573,587 - -0.3
2,573,785 + thiG OKFHMN_13395 0.26 -0.2
2,573,786 - thiG OKFHMN_13395 0.26 +0.9
2,574,089 - thiG OKFHMN_13395 0.66 +0.7
2,574,089 - thiG OKFHMN_13395 0.66 -1.0
2,574,186 + thiG OKFHMN_13395 0.78 -0.3
2,574,299 + -0.6
2,574,431 + -0.2
2,574,567 + thiH OKFHMN_13400 0.19 -1.7
2,574,619 + thiH OKFHMN_13400 0.24 -1.3
2,574,619 + thiH OKFHMN_13400 0.24 -0.3
2,574,619 + thiH OKFHMN_13400 0.24 -0.7
2,574,620 - thiH OKFHMN_13400 0.24 -0.7
2,574,766 + thiH OKFHMN_13400 0.37 -0.4
2,574,825 - thiH OKFHMN_13400 0.42 -1.0
2,574,825 - thiH OKFHMN_13400 0.42 -0.2
2,574,889 + thiH OKFHMN_13400 0.48 +0.2
2,574,901 + thiH OKFHMN_13400 0.49 -0.4
2,574,901 + thiH OKFHMN_13400 0.49 +2.5
2,574,901 + thiH OKFHMN_13400 0.49 -1.2
2,575,003 - thiH OKFHMN_13400 0.58 -0.3
2,575,007 + thiH OKFHMN_13400 0.58 +0.5
2,575,023 - thiH OKFHMN_13400 0.59 +0.9
2,575,057 + thiH OKFHMN_13400 0.62 +1.1
2,575,057 + thiH OKFHMN_13400 0.62 -0.5
2,575,058 - thiH OKFHMN_13400 0.63 +0.2
2,575,058 - thiH OKFHMN_13400 0.63 -0.1
2,575,059 + thiH OKFHMN_13400 0.63 +0.9
2,575,097 - thiH OKFHMN_13400 0.66 -1.1
2,575,111 + thiH OKFHMN_13400 0.67 -0.1
2,575,144 - thiH OKFHMN_13400 0.70 +0.0
2,575,188 - thiH OKFHMN_13400 0.74 -0.7
2,575,293 + thiH OKFHMN_13400 0.83 +0.3
2,575,294 - thiH OKFHMN_13400 0.83 +0.0

Or see this region's nucleotide sequence