Experiment: Bas69
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speE and speD are separated by 15 nucleotides speD and yacL are separated by 37 nucleotides yacL and acnB are separated by 175 nucleotides
OKFHMN_10465: speE - polyamine aminopropyltransferase, at 1,962,356 to 1,963,222
speE
OKFHMN_10470: speD - adenosylmethionine decarboxylase, at 1,963,238 to 1,964,032
speD
OKFHMN_10475: yacL - protein YacL, at 1,964,070 to 1,964,432
yacL
OKFHMN_10480: acnB - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 1,964,608 to 1,967,205
acnB
Position (kb) 1964
1965 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1963.070 kb on - strand, within speE at 1963.081 kb on - strand, within speE at 1963.081 kb on - strand, within speE at 1963.145 kb on + strand at 1963.221 kb on - strand at 1963.231 kb on + strand at 1963.298 kb on + strand at 1963.310 kb on + strand at 1963.349 kb on + strand, within speD at 1963.364 kb on + strand, within speD at 1963.365 kb on - strand, within speD at 1963.365 kb on - strand, within speD at 1963.365 kb on - strand, within speD at 1963.366 kb on + strand, within speD at 1963.421 kb on - strand, within speD at 1963.430 kb on - strand, within speD at 1963.474 kb on + strand, within speD at 1963.588 kb on + strand, within speD at 1963.588 kb on + strand, within speD at 1963.588 kb on + strand, within speD at 1963.588 kb on + strand, within speD at 1963.588 kb on + strand, within speD at 1963.588 kb on + strand, within speD at 1963.588 kb on + strand, within speD at 1963.588 kb on + strand, within speD at 1963.589 kb on - strand, within speD at 1963.589 kb on - strand, within speD at 1963.589 kb on - strand, within speD at 1963.589 kb on - strand, within speD at 1963.624 kb on + strand, within speD at 1963.625 kb on - strand, within speD at 1963.688 kb on + strand, within speD at 1963.689 kb on - strand, within speD at 1963.719 kb on + strand, within speD at 1963.811 kb on - strand, within speD at 1963.822 kb on + strand, within speD at 1963.823 kb on - strand, within speD at 1963.851 kb on + strand, within speD at 1963.851 kb on + strand, within speD at 1963.916 kb on + strand, within speD at 1963.916 kb on + strand, within speD at 1964.244 kb on - strand, within yacL at 1964.244 kb on - strand, within yacL at 1964.244 kb on - strand, within yacL at 1964.244 kb on - strand, within yacL at 1964.245 kb on + strand, within yacL at 1964.246 kb on - strand, within yacL at 1964.246 kb on - strand, within yacL at 1964.246 kb on - strand, within yacL at 1964.348 kb on + strand, within yacL at 1964.384 kb on + strand, within yacL at 1964.385 kb on - strand, within yacL at 1964.394 kb on + strand, within yacL at 1964.432 kb on + strand at 1964.486 kb on + strand at 1964.487 kb on - strand at 1964.487 kb on - strand at 1964.487 kb on - strand at 1964.487 kb on - strand at 1964.491 kb on - strand at 1964.685 kb on - strand at 1965.385 kb on - strand, within acnB at 1965.385 kb on - strand, within acnB
Per-strain Table
Position Strand Gene LocusTag Fraction Bas69 remove 1,963,070 - speE OKFHMN_10465 0.82 +0.2 1,963,081 - speE OKFHMN_10465 0.84 -0.1 1,963,081 - speE OKFHMN_10465 0.84 -0.1 1,963,145 + -0.7 1,963,221 - +0.6 1,963,231 + +0.1 1,963,298 + -0.7 1,963,310 + +0.1 1,963,349 + speD OKFHMN_10470 0.14 -0.0 1,963,364 + speD OKFHMN_10470 0.16 -1.2 1,963,365 - speD OKFHMN_10470 0.16 -0.0 1,963,365 - speD OKFHMN_10470 0.16 -0.2 1,963,365 - speD OKFHMN_10470 0.16 -0.0 1,963,366 + speD OKFHMN_10470 0.16 -0.0 1,963,421 - speD OKFHMN_10470 0.23 -2.2 1,963,430 - speD OKFHMN_10470 0.24 -2.1 1,963,474 + speD OKFHMN_10470 0.30 -0.6 1,963,588 + speD OKFHMN_10470 0.44 -0.6 1,963,588 + speD OKFHMN_10470 0.44 +1.0 1,963,588 + speD OKFHMN_10470 0.44 -1.3 1,963,588 + speD OKFHMN_10470 0.44 +0.2 1,963,588 + speD OKFHMN_10470 0.44 -0.0 1,963,588 + speD OKFHMN_10470 0.44 -0.4 1,963,588 + speD OKFHMN_10470 0.44 +1.4 1,963,588 + speD OKFHMN_10470 0.44 +0.1 1,963,589 - speD OKFHMN_10470 0.44 -0.3 1,963,589 - speD OKFHMN_10470 0.44 +0.6 1,963,589 - speD OKFHMN_10470 0.44 -1.9 1,963,589 - speD OKFHMN_10470 0.44 -1.6 1,963,624 + speD OKFHMN_10470 0.49 +0.4 1,963,625 - speD OKFHMN_10470 0.49 +0.2 1,963,688 + speD OKFHMN_10470 0.57 +0.2 1,963,689 - speD OKFHMN_10470 0.57 +0.6 1,963,719 + speD OKFHMN_10470 0.61 +0.2 1,963,811 - speD OKFHMN_10470 0.72 +0.2 1,963,822 + speD OKFHMN_10470 0.73 -0.9 1,963,823 - speD OKFHMN_10470 0.74 +0.5 1,963,851 + speD OKFHMN_10470 0.77 -0.7 1,963,851 + speD OKFHMN_10470 0.77 +0.2 1,963,916 + speD OKFHMN_10470 0.85 -1.2 1,963,916 + speD OKFHMN_10470 0.85 +0.5 1,964,244 - yacL OKFHMN_10475 0.48 -0.8 1,964,244 - yacL OKFHMN_10475 0.48 +0.5 1,964,244 - yacL OKFHMN_10475 0.48 +1.2 1,964,244 - yacL OKFHMN_10475 0.48 -1.3 1,964,245 + yacL OKFHMN_10475 0.48 +0.7 1,964,246 - yacL OKFHMN_10475 0.48 -0.5 1,964,246 - yacL OKFHMN_10475 0.48 -0.0 1,964,246 - yacL OKFHMN_10475 0.48 +1.4 1,964,348 + yacL OKFHMN_10475 0.77 +2.0 1,964,384 + yacL OKFHMN_10475 0.87 -0.4 1,964,385 - yacL OKFHMN_10475 0.87 +1.0 1,964,394 + yacL OKFHMN_10475 0.89 +0.6 1,964,432 + +1.4 1,964,486 + +0.0 1,964,487 - -1.0 1,964,487 - -0.0 1,964,487 - +0.6 1,964,487 - -0.7 1,964,491 - +2.4 1,964,685 - +1.5 1,965,385 - acnB OKFHMN_10480 0.30 -0.6 1,965,385 - acnB OKFHMN_10480 0.30 +2.0
Or see this region's nucleotide sequence