Strain Fitness in Escherichia coli ECRC100 around OKFHMN_02430

Experiment: Bas69

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpuuB and puuC are separated by 1 nucleotidespuuC and puuR are separated by 149 nucleotidespuuR and puuD are separated by 26 nucleotides OKFHMN_02425: puuB - gamma-glutamylputrescine oxidase, at 371,596 to 372,876 puuB OKFHMN_02430: puuC - aldehyde dehydrogenase PuuC, at 372,878 to 374,365 puuC OKFHMN_02435: puuR - HTH-type transcriptional regulator PuuR, at 374,515 to 375,072 puuR OKFHMN_02440: puuD - gamma-glutamyl-gamma-aminobutyrate hydrolase, at 375,099 to 375,863 puuD Position (kb) 372 373 374 375Strain fitness (log2 ratio) -1 0 1at 372.167 kb on + strand, within puuBat 372.167 kb on + strand, within puuBat 372.192 kb on - strand, within puuBat 372.220 kb on - strand, within puuBat 372.855 kb on - strandat 373.009 kb on + strandat 374.085 kb on - strand, within puuCat 374.116 kb on + strand, within puuCat 374.116 kb on + strand, within puuCat 374.282 kb on - strandat 374.393 kb on + strandat 374.395 kb on + strandat 374.396 kb on - strandat 374.517 kb on - strandat 374.599 kb on - strand, within puuRat 374.655 kb on + strand, within puuRat 374.701 kb on - strand, within puuRat 374.819 kb on - strand, within puuRat 374.897 kb on + strand, within puuRat 374.898 kb on - strand, within puuRat 374.953 kb on + strand, within puuRat 374.965 kb on - strand, within puuR

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas69
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372,167 + puuB OKFHMN_02425 0.45 -0.7
372,167 + puuB OKFHMN_02425 0.45 +0.7
372,192 - puuB OKFHMN_02425 0.47 +0.4
372,220 - puuB OKFHMN_02425 0.49 +0.8
372,855 - +0.3
373,009 + -0.1
374,085 - puuC OKFHMN_02430 0.81 +0.8
374,116 + puuC OKFHMN_02430 0.83 -0.5
374,116 + puuC OKFHMN_02430 0.83 -0.1
374,282 - +1.5
374,393 + -0.1
374,395 + -0.5
374,396 - -1.5
374,517 - -0.8
374,599 - puuR OKFHMN_02435 0.15 +1.1
374,655 + puuR OKFHMN_02435 0.25 +0.1
374,701 - puuR OKFHMN_02435 0.33 +1.5
374,819 - puuR OKFHMN_02435 0.54 +0.8
374,897 + puuR OKFHMN_02435 0.68 +0.7
374,898 - puuR OKFHMN_02435 0.69 +0.5
374,953 + puuR OKFHMN_02435 0.78 +0.8
374,965 - puuR OKFHMN_02435 0.81 -0.7

Or see this region's nucleotide sequence